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Nanometer porphyrin catalyst and method for catalyzing TMB color development by using nanometer porphyrin catalyst

A catalyst and porphyrin technology, applied in the field of analysis and detection, can solve the problems of harsh synthesis process conditions and high cost of raw materials, and achieve the effects of overcoming instability, high catalytic effect, and excellent peroxidase-like activity.

Active Publication Date: 2019-04-26
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Nanomaterials that combine metal oxides with metalloporphyrins have shown high peroxidase-like activity, such as: metal oxide-porphyrin (NiO-H 2 TCPP), CNPs-porphyrin, H 2 TCPP-CeO 2 NPs, H 2 TCPP-MoS 2 NPs have been reported, but these porphyrin-metal / metal oxide nanomaterials still have the disadvantages of harsh synthesis process conditions, expensive raw material costs, and the synthesis of toxic organic reagents. Therefore, the development of a highly operable and Low-cost pseudoperoxidases are crucial

Method used

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  • Nanometer porphyrin catalyst and method for catalyzing TMB color development by using nanometer porphyrin catalyst
  • Nanometer porphyrin catalyst and method for catalyzing TMB color development by using nanometer porphyrin catalyst
  • Nanometer porphyrin catalyst and method for catalyzing TMB color development by using nanometer porphyrin catalyst

Examples

Experimental program
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Effect test

Embodiment 1

[0033] A kind of nanoporphyrin catalyst, its preparation method comprises the steps:

[0034] Dilute 9.5mL to a concentration of 2.63×10 -3 M with 500 μL of DTAB aqueous solution at a concentration of 1 x 10 -5 M ZnTPyP mixed with N,N-dimethylformamide solution, ultrasonic (100W, 25°C) for 10-15min, then heated to 70°C in a water bath for 15-20min to initiate a self-assembly reaction to obtain a nanoporphyrin catalyst (DTAB-ZnTPyP ).

[0035] The nano-porphyrin catalyst obtained in this embodiment is used to catalyze TMB color development, and the specific steps include:

[0036] Dilute 100 μL to a concentration of 5 x 10 -7 M nanoporphyrin, added to 100 μL of N,N-dimethylformamide solution with a concentration of 3 mM TMB, 100 μL with a concentration of 30% H 2 o 2 solution, 700μL acetate buffer solution (0.2M, pH=3.6) to form the mixed chromogenic system I (TMB+H 2 o 2 +DTAB-ZnTPyP), incubated at 40°C for 8min, the color response of the solution system is as follows ...

Embodiment 2

[0041] A kind of nanoporphyrin catalyst, its preparation method comprises the steps:

[0042] Dilute 9.5mL to a concentration of 2.63×10 -2 M DTAB aqueous solution with 500 μl concentration of 1 x 10 -5M ZnTPyP mixed with N,N-dimethylformamide solution, ultrasonic (100W, 25°C) for 10-15min, then heated to 70°C in a water bath for 15-20min to initiate a self-assembly reaction to obtain a nanoporphyrin catalyst (DTAB-ZnTPyP ).

[0043] figure 2 This is the electron micrograph of the nano-porphyrin catalyst obtained in this example. It can be seen from the figure that the obtained product has a nano-rod structure with a particle size of 70-80 nm.

Embodiment 3

[0045] A kind of method utilizing nano porphyrin catalyst to catalyze TMB color development, comprises the steps:

[0046] To a concentration of 5 x 10 containing 100 μL -7 M's nano-porphyrin aqueous solution (prepared in Example 2), 100 μL 3×10 -3 N,N-dimethylformamide solution of MTMB and 100 μL of 30% H 2 o 2 Add 700 μL of 0.2M acetate buffer solution with different pH values ​​(pH is 2.0, 3.6, 4.0, 4.5, 6, 7) to the six mixed solutions of the aqueous solution, incubate the reaction at 40°C for 8 minutes, and carry out ultraviolet Scanning (at 652nm) results such as image 3 shown.

[0047] The results showed that when the pH value of the acetate buffer solution was in the range of 2.0-4.5, the nano-porphyrins could show a good catalytic effect on TMB, and the best catalytic performance was shown at pH=3.6.

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Abstract

The invention discloses a nanometer porphyrin catalyst. The nanometer porphyrin catalyst takes a cationic surface active agent and ZnTPyP as main raw materials, and is formed through water bath self-assembly; the nanometer porphyrin catalyst has better peroxidase activity and can be effectively utilized to catalyze and oxidize 3,3',5,5'-tetramethylbenzidine (TMB) to generate a colorless-to-blue color development reaction. The nanometer porphyrin catalyst has the advantages that rapid color development is achieved, the catalytic efficiency is high, the synthetic cost is low, and the catalytic effect of DTAB self-assembled ZnTPyP is higher than that of a pure surface active agent DTAB and much higher than that of the ZnTPyP, and the nanometer porphyrin catalyst has an important application prospect.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection, and in particular relates to a method for developing color of TMB catalyzed by nano-porphyrin. Background technique [0002] Peroxidases catalyze many chemical reactions due to the hydrogen peroxide-dependent one-electron oxidation of various substrates and are useful as components of clinical diagnostic kits, in immunoassays, etc. The most widespread enzyme in analytical biochemistry is horseradish peroxidase (HRP), which is used to construct biosensors and immunoassays. However, the inherent instability of natural enzymes, high-cost purification process, sources, etc. limit their practical applications. Based on the shortcomings of natural enzymes, the development of more and more artificial enzymes has become an urgent need for sensitive enzyme mimics recently. [0003] With Fe 3 o 4 The peroxidase properties of magnetic nanoparticles have been reported, and a series of meta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/33
CPCG01N21/33G01N21/78
Inventor 杨小龙时琼付海燕杨天鸣
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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