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Vegetable oil degumming method

A vegetable oil and degumming technology, applied in the field of vegetable oil degumming, can solve the problems of low degumming efficiency and achieve the effect of continuous production, high-efficiency separation and improved yield

Pending Publication Date: 2019-04-30
HEFEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the deficiencies of the prior art, the present invention provides a method for degumming vegetable oil, which solves the technical problem of low degumming efficiency in the prior art by using a single enzyme for degumming

Method used

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preparation example Construction

[0043] Preparation of immobilized phospholipase C: Weigh the cross-linked Fe 3 o 4 50 mg of the composite carrier was put into an Erlenmeyer flask, and 8.0 mL of 0.02 g / mL phospholipase C solution (diluted with pH 6.7 phosphate buffer) was added. In a water-bath shaker, react at 30°C and 150r / min for 7.9h. Subsequently, the immobilized phospholipase C was separated with a permanent magnet, and washed repeatedly with phosphate buffer (pH=7.0) until no free enzyme was detected in the supernatant. The immobilized phospholipase C was prepared.

[0044] immobilized phospholipase A 1 Preparation: Take 0.2 g of the pretreated macroporous resin, place it in a 250 mL Erlenmeyer flask, add 6.0 mL of phospholipase A diluted with 0.1 mol / L, pH 7.0 phosphate buffer 1 The enzyme solution was immobilized in a water-bath shaker at 25°C for 6.0 hours, then added a certain amount of glutaraldehyde solution to cross-link for 8.0 hours, and finally washed several times with phosphate buffer u...

Embodiment 1

[0057] Embodiments of the present invention provide a method for degumming rapeseed oil, comprising the following steps:

[0058] S1: phospholipase immobilization;

[0059] Phospholipase A 1 The immobilization of and the immobilization of phospholipase C;

[0060] Preparation of immobilized phospholipase C: Weigh the cross-linked Fe 3 o 4 50 mg of the composite carrier was put into an Erlenmeyer flask, and 6.0 mL of 0.02 g / mL phospholipase C solution (diluted with pH 6.7 phosphate buffer) was added. In a water-bath shaker, react for 7.0 h at 35° C. and 160 r / min. Subsequently, the immobilized phospholipase C was separated with a permanent magnet, and washed repeatedly with phosphate buffer (pH=7.0) until no free enzyme was detected in the supernatant. The immobilized phospholipase C was prepared.

[0061] immobilized phospholipase A 1 Preparation: Take 0.2 g of the pretreated macroporous resin, place it in a 250 mL Erlenmeyer flask, add 6.0 mL of phospholipase A diluted...

Embodiment 2

[0070] The embodiment of the present invention provides a kind of method of soybean oil degumming, comprises the following steps:

[0071] S1: phospholipase immobilization;

[0072] Phospholipase A 1 The immobilization of and the immobilization of phospholipase C;

[0073] Preparation of immobilized phospholipase C: Weigh the cross-linked Fe 3 o 4 50 mg of the composite carrier was put into an Erlenmeyer flask, and 8.0 mL of 0.02 g / mL phospholipase C solution (diluted with pH 6.7 phosphate buffer) was added. In a water-bath shaker, react for 8.0 hours at 25°C and 150r / min. Subsequently, the immobilized phospholipase C was separated with a permanent magnet, and washed repeatedly with phosphate buffer (pH=7.0) until no free enzyme was detected in the supernatant. The immobilized phospholipase C was prepared.

[0074] immobilized phospholipase A 1 Preparation: Take 0.2 g of the pretreated macroporous resin, place it in a 250 mL Erlenmeyer flask, add 6.0 mL of phospholipase...

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Abstract

The invention provides a vegetable oil degumming method, and relates to the technical field of enzymatic degumming of oils and fats. The method comprises the following steps: 1, immobilizing phospholipase; and 2, degumming oil by the immobilized phospholipase. The immobilization of the phospholipase includes immobilization of phospholipase A1 and immobilization of phospholipase C; and degumming ofoil comprises pretreatment of vegetable oil, primary degumming of the vegetable oil by using the immobilized phospholipase C; and secondary degumming of the primarily degummed vegetable oil by usingthe immobilized phospholipase A1. The method adopts an immobilized phospholipase C and phospholipase A1 combined degumming technology, and the degumming effects of the two enzymes on the oil are fullyshown, so the yield of the neutral oil is increased; and the immobilized phospholipase can be repeatedly used, so the production cost is saved.

Description

technical field [0001] The invention relates to the technical field of grease enzymatic degumming, in particular to a method for vegetable oil degumming. Background technique [0002] Crude oil extracted from oilseeds by pressing, leaching, etc. contains impurities such as proteins, sugars, phospholipids, pigments, metal ions, free fatty acids, etc., and is usually not edible directly. It needs to go through a series of oil refining processes to remove impurities before it can be obtained. Edible oil that meets national requirements. The phospholipids, proteins and mucous substances contained in crude oil are collectively called colloids, and the colloids in crude oil are mainly phospholipids, so degumming is also called dephosphorization. To meet the requirements of physical refining, the phosphorus content of oil must be controlled within 10mg / kg during the degumming process, otherwise the phospholipids will easily coke at high temperature after entering the deodorization...

Claims

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Application Information

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IPC IPC(8): C11B3/00
CPCC11B3/003
Inventor 操丽丽姜绍通李进红庞敏侯志刚
Owner HEFEI UNIV OF TECH
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