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Preparation method of solid bacterial agent for hemp bio-degumming

A technology of biological degumming and solid-state bacterial agent, which is applied in the direction of bacteria and bacterial retting, etc., can solve the problems of easy degradation of bacterial strains, expansion of variation and application, difficulty in strain preparation technology, etc., achieving great theoretical and practical significance, and easy to popularize and apply , The effect of promoting bacterial sedimentation

Active Publication Date: 2019-04-30
INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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Problems solved by technology

[0005] However, the DCE-01 strain is a heat-sensitive Gram-negative bacterium, and its strain preparation technology is relatively difficult, especially the technical problems that the strain is easy to degrade and mutate, which restricts its further expanded application in the biological degumming of hemp

Method used

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  • Preparation method of solid bacterial agent for hemp bio-degumming

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Embodiment 1

[0031] Embodiment 1 A kind of preparation method of solid bacterial agent for biological degumming of hemp

[0032] Such as figure 1 As shown, a ring of DCE-01 bacterial strains was picked from the slant original seed lawn preserved by the research group of Professor Peng Yuande, Chinese Academy of Agricultural Sciences, and inoculated into 5 mL of improved broth culture medium (GR culture medium), fully suspended, and 35 Incubate at ℃ for 6 hours; spread it on YQ nutrient agar plate after dilution, incubate at 35℃ for 20 hours, and isolate a single colony; select a typical colony and streak it on a fresh slope, incubate at 35℃ for 20 hours, vacuum pack, and store at room temperature; put 1mL of sterilized improved Pour the broth culture solution (GR culture solution) into one tube of the above-mentioned slanted bacteria, and gently scrape the bacterial lawn on the surface with an inoculation loop to ensure that the bacterial lawn is completely mixed into the culture solution....

Embodiment 2

[0042] Embodiment 2 A kind of preparation method of solid bacterial agent for biological degumming of hemp

[0043] Pick a ring of DCE-01 strains from the slant original species preserved by the research group of Professor Peng Yuande of the Institute of Hemp, Chinese Academy of Agricultural Sciences, and inoculate it into 5mL of improved broth culture medium (GR culture medium), suspend well, and incubate at 35°C for 6h After dilution, spread on YQ nutrient agar plate, culture at 35°C for 18 hours, and isolate a single colony; select typical colonies and streak on a fresh slope, culture at 35°C for 18 hours, vacuum pack, and store at room temperature; culture 1 mL of sterilized improved broth Liquid (GR culture medium) was poured into 1 tube of the above-mentioned slant strains, and the bacterial lawn on the surface was gently scraped with an inoculation loop to ensure that the bacterial lawn was completely mixed into the culture medium.

[0044] Specifically, the mass ratio ...

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Abstract

The invention discloses a preparation method of a solid bacterial agent for hemp bio-degumming, wherein the preparation method includes the steps: inoculating an FJ fermentation culture liquid with anactivated DCE-01 seed liquid, carrying out expansion culture and fermentation to obtain a mature fermentation liquid, and then collecting a wetting bacterial body; followed by, adding a protective agent onto the wetting bacterial body, and mixing evenly to obtain wetting bacterial powder; and finally, flatly laying the wetting bacterial powder and baking at the temperature of 32-35 DEG C to obtain the product. Corn pulp dry powder and soybean meal powder are used for replacing most peptone to prepare the FJ fermentation culture liquid, the production cost is reduced, and the flocculation effect is achieved; sterilized bran is selected as a protective agent, the thalli adsorption capacity is large, and the load of subsequent drying is reduced; by adopting of a cold air and atmospheric pressure drying method, the DCE-01 strain is protected from injury to the greatest extent, the energy is saved, and the theoretical and practical significance is great.

Description

technical field [0001] The invention relates to the technical field of processing hemp raw materials, in particular to a preparation method of a solid bacterial agent for biological degumming of hemp. Background technique [0002] Hemp bast raw material is one of the important sources of natural fibers in the world. In addition to a large amount of cellulose fibers, the hemp bast raw materials of agricultural products also contain "gum" components such as pectin, hemicellulose, and lignin. No matter what kind of textile products are developed by using hemp bast fiber, it must go through "degumming" to remove the non-cellulosic substances of the bast fiber raw material. Therefore, degumming is the basic and critical link in the processing of hemp bast fiber raw materials. Hemp biological degumming is favored because of its "high efficiency, energy saving, and low emission". [0003] The core of biological degumming is high-efficiency strains. A series of biological degumm...

Claims

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Application Information

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IPC IPC(8): C12N1/20D01C1/04
CPCC12N1/20D01C1/04
Inventor 彭源德成莉凤段盛文冯湘沅郑科杨琦刘志远
Owner INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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