Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing hemoglobin peptide rare earth cerium chelate

A technology of hemoglobin peptide and rare earth cerium, which is applied in the field of preparation of rare earth chelate, can solve the problems that the preparation technology of rare earth cerium chelate of hemoglobin peptide has not been found, and achieve the effect of low price, simple process and low cost

Inactive Publication Date: 2019-04-30
陈石良
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, domestic research and development of rare earth chelates of protein peptides are few, and no domestic public literature reports on the preparation technology of rare earth cerium chelates of hemoglobin peptides have been found.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing hemoglobin peptide rare earth cerium chelate
  • Method for preparing hemoglobin peptide rare earth cerium chelate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Preparation of porcine blood cells: collect 1000L of fresh pig blood that has been anticoagulated, and use a tube centrifuge to continuously centrifuge to obtain 455L of blood cells and 545L of plasma, collect the blood cells, and spray the plasma to make plasma protein powder ;

[0024] (2) Hemolysis: pump the blood cell solution into the reaction tank, add 450L of deionized water, and stir continuously for 60 minutes to break the blood cells. The stirring speed is 30r / min to prepare 900L of hemolysis;

[0025] (3) Enzymolysis: Heating the hemolyzate to 50°C with continuous stirring, adjusting the pH value to 8.5 with NaOH solution, then adding protease at 1.2% of the weight of the blood cell solution, stirring continuously, and detecting the enzymolysis solution at intervals of one hour pH value, when the pH value drops below 8.5, adjust the pH value to 8.5 with alkali supplementation solution, heat the enzymolysis solution for 6 hours, heat the enzymolysis soluti...

Embodiment 2

[0031] (1) Preparation of pig blood cells: collect 1000L of fresh pig blood that has been anticoagulated, and use a tubular centrifuge to continuously centrifuge to obtain 430L of blood cells and 570L of plasma. ;

[0032] (2) Hemolysis: pump the blood cell solution into the reaction tank, add 430L of deionized water, and stir continuously for 60 minutes to break the blood cells. The stirring speed is 30r / min to prepare 850L of hemolysis;

[0033] (3) Enzymolysis: Heat the hemolyzate to 55°C with continuous stirring, adjust its pH value to 8.2 with NaOH solution, then add protease at 2% of the weight of the blood cell solution, keep stirring, and check the enzymolysis solution at intervals of one hour PH value, when the pH value drops below 8.2, adjust the pH value to 8.2 with alkali supplementation solution, keep the enzymatic hydrolysis for 4 hours, heat the enzymatic hydrolysis solution to 85°C to inactivate the enzyme for 10 minutes, and obtain the enzymatic hydrolysis sol...

Embodiment 3

[0039] (1) Preparation of porcine blood cells: collect 1000L of fresh pig blood that has been anticoagulated, and use a tubular centrifuge to continuously centrifuge to obtain 410L of blood cells and 590L of plasma, collect the blood cells, and spray dry the plasma to make plasma protein powder ;

[0040] (2) Hemolysis: pump the blood cell solution into the reaction tank, add 400L of deionized water, and stir continuously for 60 minutes to break the blood cells. The stirring speed is 30r / min, and 800L of hemolysis is obtained;

[0041] (3) Enzymolysis: Heating the hemolyzate to 52°C with continuous stirring, adjusting the pH value to 8.0 with NaOH solution, then adding 1.5% protease by weight of the blood cell solution, stirring constantly, and detecting the enzymatic hydrolyzate at intervals of one hour pH value, when the pH value drops below 8.0, adjust the pH value to 8.0 with alkali supplementation solution, heat the enzymolysis solution for 5 hours, heat the enzymolysis s...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for preparing a hemoglobin peptide rare earth cerium chelate. The main steps include pig blood cell liquid pulverization, hemolysis, enzymatic hydrolysis, centrifugation, ultrafiltration, chelation reaction, and spray drying to obtain the hemoglobin peptide rare earth cerium chelate. The method of the invention adopts a low-cost and widely-sourced pig blood as a protein peptide source, and uses rare earth cerium salt as a cerium source for chelating cerium element with protein peptide, and the method has a simple process flow and low cost, and is suitable forlarge-scale industrial production. The product prepared by the invention has high micromolecule peptide content and high chelating rate, and is a novel high-efficiency feed additive.

Description

technical field [0001] The invention relates to a preparation method of a rare earth chelate, in particular to a preparation method of a hemoglobin peptide rare earth cerium chelate. Background technique [0002] Animal nutrition research results show that animals absorb most of the trace elements in the form of chelates, and most of the trace element metal ions in the animal body exist in the form of protein salts. Therefore, trace element chelates are developing rapidly at home and abroad as the third generation of new trace element additives. According to the different raw materials of chelating agents, trace element chelates include amino acid chelates, protein peptide chelates and other types. Protein peptide trace element chelate is a compound with a ring structure formed by the coordination reaction between trace element metal ions and small molecule peptides or short peptides. Protein peptide trace element chelate is a form of salt that is close to the natural form...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K14/805C07K1/34C07K1/113
CPCC12P21/06C07K14/805
Inventor 陈石良
Owner 陈石良
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com