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Vaccine adjuvant and application thereof as well as vaccine against porcine reproductive and respiratory syndrome

A respiratory syndrome and vaccine adjuvant technology, applied in the field of porcine reproductive and respiratory syndrome vaccine and vaccine adjuvant, can solve the problems of weak cellular immune response and unsatisfactory immune protection effect, so as to reduce viremia and protect immune system. Damaged, reduced immunosuppressive effects

Inactive Publication Date: 2019-05-03
龙阔(苏州)生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Although the inactivated PRRS vaccine is safe, it only induces a very weak cellular immune response, and its immune protection effect is not ideal

Method used

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  • Vaccine adjuvant and application thereof as well as vaccine against porcine reproductive and respiratory syndrome
  • Vaccine adjuvant and application thereof as well as vaccine against porcine reproductive and respiratory syndrome
  • Vaccine adjuvant and application thereof as well as vaccine against porcine reproductive and respiratory syndrome

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A kind of vaccine adjuvant, antibacterial peptide, methionine, sodium glycinecholine and stabilizer are added into deionized water, it is advisable to stir at the maximum speed without foaming, and then add aluminum hydroxide glue after mixing to obtain Water-soluble suspension (volume is 2mL), the concentration of antimicrobial peptide in this water-soluble suspension is 0.25mg / mL, the concentration of methionine is 5mg / mL, the concentration of sodium glycinecholine is 0.5mg / mL , the concentration of the stabilizer is 1.5g / mL; the mass fraction of aluminum hydroxide gel in the water-soluble suspension is 20%.

[0027] The pH value of the vaccine adjuvant in this example is 6.8.

[0028] The porcine reproductive and respiratory syndrome vaccine of this embodiment is prepared by mixing the above-mentioned vaccine adjuvant with porcine reproductive and respiratory syndrome virus antigen at a ratio of 1:1.

Embodiment 2

[0030] A kind of vaccine adjuvant, antibacterial peptide, methionine, sodium glycinecholine and stabilizer are added into deionized water, it is advisable to stir at the maximum speed without foaming, and then add aluminum hydroxide glue after mixing to obtain Water-soluble suspension (volume is 2mL), the concentration of the antibacterial peptide in this water-soluble suspension is 0.5mg / mL, and the concentration of methionine is 0.5mg / mL, and the concentration of sodium glycinecholine is 1.5mg / mL mL, the concentration of the stabilizer is 0.5g / mL; the mass fraction of aluminum hydroxide gel in the water-soluble suspension is 10%.

[0031] The pH value of the vaccine adjuvant in this example is 7.5.

[0032] The porcine reproductive and respiratory syndrome vaccine of this embodiment is prepared by mixing the above-mentioned vaccine adjuvant with porcine reproductive and respiratory syndrome virus antigen at a ratio of 1:1.

Embodiment 3

[0034] A kind of vaccine adjuvant, antibacterial peptide, methionine, sodium glycinecholine and stabilizer are added into deionized water, it is advisable to stir at the maximum speed without foaming, and then add aluminum hydroxide glue after mixing to obtain Water-soluble suspension (volume is 2mL), the concentration of the antimicrobial peptide in this water-soluble suspension is 1.5mg / mL, and the concentration of methionine is 2.5mg / mL, and the concentration of sodium glycinecholine is 2.5mg / mL. mL, the concentration of the stabilizer is 1g / mL; the mass fraction of aluminum hydroxide gel in the water-soluble suspension is 20%.

[0035] The pH value of the vaccine adjuvant in this example is 7.8.

[0036] The porcine reproductive and respiratory syndrome vaccine of this embodiment is prepared by mixing the above-mentioned vaccine adjuvant with porcine reproductive and respiratory syndrome virus antigen at a ratio of 1:1.

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Abstract

The invention is applied to the field of medicine biotechnology, and provides a vaccine adjuvant and application thereof as well as a vaccine against a porcine reproductive and respiratory syndrome. The vaccine adjuvant is water-soluble suspension liquid which is obtained by adding antimicrobial peptides, aluminium hydroxide gel, methionine, citicoline sodium glycine and a stabilizing agent into deionized water, wherein in the water-soluble suspension liquid, the concentration of the antimicrobial peptides is 0.5mg / mL to 2.5mg / mL, the concentration of methionine is 0.5mg / mL to 5mg / mL, the concentration of the citicoline sodium glycine is 0.5mg / mL to 5mg / mL, and the concentration of the stabilizing agent is 0.5mg / mL to 1.5mg / mL; the aluminium hydroxide gel is 10 percent by mass to 20 percent by mass of the water-soluble suspension liquid. By adopting the vaccine adjuvant disclosed by the invention, the differentiation proportion of M1 to M2 in macrophages can be induced, different typesof PRRSV (Porcine Reproductive and Sespiratory Syndrome Virus) virus strains can be killed, and meanwhile, internal organs are protected from being hurt. With combination of PRRSV killed antigens, the cellular immunity of organism can be improved, interferon is induced, and immunodepression produced by the PRRSV is reduced, so that a neutral antibody with high content can be produced quickly, themaintenance time is long, and viraemia of different virus strains can be reduced.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a vaccine adjuvant and its application and a porcine reproductive and respiratory syndrome vaccine. Background technique [0002] Porcine reproductive and respiratory syndrome (PRRS, Porcine Reproductive and Respiratory Syndrome) is a highly contagious disease in pigs. The pathogen is porcine reproductive and respiratory syndrome virus. The main host of the virus is domestic pigs and wild boars in nature. . Pigs of different ages, breeds and genders can be infected with PRRSV (Porcine Reproductive and Respiratory Syndrome Virus), among which pregnant sows and suckling piglets are more seriously affected. In addition to infecting animals, boar semen has also been identified as a common source of PRRSV infection. [0003] The current common method of preventing and controlling PRRS is immunization and vaccination. The commonly used PRRS vaccines in the world mainly include...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61K39/12A61P31/14
Inventor 赖志高俊锋韩相敏龙进学马晶晶吴碧清王杰
Owner 龙阔(苏州)生物工程有限公司
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