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Tissue culture and rapid propagation method for ilex purpurea superior plant

A technology for tissue culture and rapid propagation of holly, which is applied in horticultural methods, botany equipment and methods, horticulture, etc., and can solve the problems that cannot meet the production requirements of fine varieties, unfavorable cultivation of holly tissue culture seedlings, unfavorable rapid propagation of holly, etc. problem, achieve the effect of reducing the possibility of seedling pollution, increasing the rooting rate and transplanting survival rate, and facilitating the transplanting speed

Active Publication Date: 2019-05-10
CHONGQING ACADEMY OF FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the medicinal materials of holly holly mainly come from wild resources, and the content of medicinal components of each plant varies greatly, which cannot be supplied stably, which is not enough to meet the needs of the pharmaceutical industry.
At present, Ilex is mainly propagated by seeds and cuttings. The propagation efficiency of the above two methods is low and cannot meet the production requirements for rapid propagation of fine varieties; occasionally, it is propagated by tissue culture
However, the tissue culture propagation method of the prior art may have high pollution rate, difficult rooting, slow growth, low survival rate of tissue culture seedlings, etc. when breeding and regenerating plants, which is unfavorable for the cultivation of holly holly tissue culture seedlings.
[0004] The patent application number is 201410478843.2 for the invention patent "a method of tissue culture and propagation of golden leaf tortoise shell holly", through disinfection and sterilization, primary culture, subculture, strong seedling culture, rooting culture, seedling hardening, transplanting steps, etc. to propagate golden leaf Tortoiseshell holly can improve the propagation coefficient of golden leaf tortoiseshell holly, accelerate the propagation speed of golden leaf tortoiseshell holly, improve the survival rate of golden leaf tortoiseshell holly, but the propagation method of golden leaf tortoiseshell holly can not be used for the propagation of red fruit holly, and There are many reproduction steps and the cycle is too long, which is not conducive to the rapid reproduction of Ilex spp., and there is no mention of the problem of reducing the pollution rate during the tissue culture process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: tissue culture rapid propagation method one:

[0035] A. Obtaining explants: Select 200 young shoots of the high-quality female plants of Ilex chinensis that year, cut off the excess leaves, and leave the bud tips, wash them with clean water, then wash them with detergent, and then rinse them with running water for 4 minutes. Wash with detergent, then disinfect with 75wt% alcohol for 23s, clean the alcohol with sterile water and filter dry, then sterilize with 0.1wt% mercuric acid solution for 8 minutes, wash and filter the mercuric solution with sterile water to obtain sterile explants;

[0036] B. Induction culture: transfer the sterile explants to the induction medium for budding, the induction medium is made of MS medium+NAA0.2mg / L+6BA 1.5mg / L+agar 6.5g / L+sucrose 30g / L , pH=5.8, cultivated for 40 days under the conditions of a culture temperature of 25°C, a light intensity of 1800lx, and a light time of 12h / d to obtain induced sprouts;

[0037] C. Pr...

Embodiment 2

[0041] Embodiment 2: tissue culture rapid propagation method two:

[0042] A. Obtain explants: choose 200 young shoots of the high-quality female plants of Ilex red fruit identical to those in Example 1, cut off the excess leaves, leave the bud tips, wash them with clean water, and then clean them with detergent. Rinse with running water for 3 minutes to clean the detergent, then disinfect with 75wt% alcohol for 20 seconds, wash the alcohol with sterile water and filter dry, then sterilize with 0.1wt% mercury chloride solution for 8 minutes, and use sterile water to dissolve the mercury The solution was washed and filtered dry to obtain sterile explants;

[0043] B. Induction culture: transfer the sterile explants to the induction medium for budding, the induction medium is made of MS medium+NAA0.2mg / L+6BA 1.5mg / L+agar 6.5g / L+sucrose 30g / L , pH=5.8, cultivated for 30 days under the conditions of a culture temperature of 25°C, a light intensity of 1800lx, and a light time of 1...

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Abstract

The invention discloses a tissue culture and rapid propagation method for an ilex purpurea superior plant and belongs to the technical field of plant tissue culture. The method comprises the followingsteps: obtaining an explant; selecting an annual tender shoot tip of the ilex purpurea superior plant, and cleaning, disinfecting and sterilizing the same; induced culture: transferring the explant into an induced culture medium to induce new buds; enrichment culture: transferring the new buds to an enrichment medium to be cultured for 30 days to obtain the proliferated shoot buds; rooting culture: slicing the shoot tips of the shoot buds and transferring the shoot tips into a rooting culture medium to be cultured for 15 days to obtain rooting seedlings; and acclimatization and transplant: sealing the rooting seedlings for 2-3 days and acclimatizing the rooting seedlings with a film for 10 days and then transplanting the rooting seedlings. The tissue culture and rapid propagation method disclosed by the invention improves the propagation coefficient of ilex purpurea, shortens the rooting culture period greatly and improves the propagation speed. Compared with a conventional ilex purpurea tissue culture method, the method provided by the invention has higher survival rate and higher adaptability.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for tissue culture and rapid propagation of superior strains of holly holly. Background technique [0002] Ilex purpurea Hassk is an evergreen tree of the genus Ilex in the holly family (Aquifoliaceae). Red fruit holly has both medicinal value and ornamental value. The Chinese Pharmacopoeia clearly records that the leaves of red fruit holly can be used as medicine. It has great medicinal value and is also the main component of the commonly used Chinese patent medicine Jizhi syrup formula. Red fruit holly has dioecious plants, and the female plant has many red fruits after autumn, which is bright and beautiful, and has a long fruit-hanging period, which can last from October of the current year to April to May of the following year. It is a landscape tree species with high ornamental value. [0003] Pelicin glucoside is a kind of chemical substance with high...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 朱恒星戴前莉卢敏唐佳佳黄飞逸魏立本
Owner CHONGQING ACADEMY OF FORESTRY SCI
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