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A simple and rapid method for producing phb/bacterial cellulose composites

A technology of bacterial cellulose and composite materials, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem of single method, and achieve simple operation, low cost, and environmental friendliness

Active Publication Date: 2022-06-17
WUHAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are few reports on the combination of bacterial cellulose and PHB. At present, the method used for combining the two is relatively simple, that is, soaking the purchased bacterial cellulose in a solution of dissolving PHB in chloroform to obtain a composite of PHB / bacterial cellulose Material

Method used

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  • A simple and rapid method for producing phb/bacterial cellulose composites
  • A simple and rapid method for producing phb/bacterial cellulose composites
  • A simple and rapid method for producing phb/bacterial cellulose composites

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A simple and rapid method for producing PHB / bacterial cellulose composite material, comprising the following steps:

[0027] (1) Microbial culture: the Ralstonia eutropha H16 (DSN428) strain stored in the freeze-dried tube was activated in the seed medium, and after 24 hours of growth, with 5% of the inoculum, the obtained bacterial liquid was inoculated into the basal medium I Expanded culture to OD 600 If the value is 1, with the same inoculum amount, inoculate the bacterial liquid into the basal medium I again and repeat the expansion culture to its OD. 600 value is 1;

[0028] (2) the Gluconacetobacter xylinus ATCC700178 strain stored in the freeze-drying tube is activated in the basal medium II, after 48h of growth, the obtained bacterial liquid is inoculated into the basal medium II and expanded for 24h, and the inoculation amount is 5%, Take the bacterial liquid of the same inoculum amount and inoculate it into the basal medium II again for repeated expansion for...

Embodiment 2

[0035] A simple and rapid method for producing PHB / bacterial cellulose composite material, comprising the following steps:

[0036] (1) Microbial culture: the Ralstonia eutropha H16 (DSM428) strain stored in the freeze-dried tube was activated in the seed medium, and after 24 hours of growth, the obtained bacterial liquid was inoculated into the basal medium I with a 5% inoculation amount Expanded culture to OD 600 If the value is 1, with the same inoculum amount, inoculate the bacterial liquid into the basal medium I again and repeat the expansion culture to its OD. 600 value is 1;

[0037] (2) the Gluconacetobacter xylinus ATCC700178 strain stored in the freeze-drying tube is activated in the basal medium II, after 48h of growth, the obtained bacterial liquid is inoculated into the basal medium II and expanded for 24h, and the inoculation amount is 5%, Take the bacterial liquid of the same inoculum amount and inoculate it into the basal medium II again for repeated expansi...

Embodiment 3

[0045] A simple and rapid method for producing PHB / bacterial cellulose composite material, comprising the following steps:

[0046] (1) Microbial culture: the Ralstonia eutropha H16 (DSM428) strain stored in the freeze-dried tube was activated in the seed medium, and after 24 hours of growth, the obtained bacterial liquid was inoculated into the basal medium I with a 5% inoculation amount Expanded culture to OD 600 If the value is 1, with the same inoculum amount, inoculate the bacterial liquid into the basal medium I again and repeat the expansion culture to its OD. 600 value is 1;

[0047] (2) the Gluconacetobacter xylinus ATCC700178 strain stored in the freeze-drying tube is activated in the basal medium II, after 48h of growth, the obtained bacterial liquid is inoculated into the basal medium II and expanded for 24h, and the inoculation amount is 5%, Take the bacterial liquid of the same inoculum amount and inoculate it into the basal medium II again for repeated expansi...

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Abstract

The invention belongs to the field of biological materials, and in particular relates to a simple and fast method for producing PHB / bacterial cellulose composite materials, comprising: 1) cultivation of Ralstonia eutropha bacterial liquid; 2) cultivation of Gluconacetobacter xylinus bacterial liquid: 3) taking Ralstonia eutropha bacteria The solution is added to the basic culture solution containing Gluconacetobacter xylinus cells, dynamic culture and static culture are carried out, the bacteria solution is collected, and the bacterial cellulose / PHB composite product is extracted. The method of the invention can produce the required composite material by directly adding an appropriate amount of Ralstonia eutropha bacterial liquid in the cultivation process of Gluconacetobacter xylinus, and the method has the advantages of simple operation, low cost, mild condition, environment friendly and the like.

Description

technical field [0001] The invention belongs to the field of biological materials, in particular to a method for simply and rapidly producing PHB / bacterial cellulose composite materials. Background technique [0002] The development of new materials is a priority scientific field in the 21st century. Bacterial cellulose (BC) is one of the research hotspots of new biomaterials. Using microbial fermentation to produce bacterial cellulose and applying it to medicine has broad application prospects in medicine, papermaking, biomedical engineering and food industry. Among them, the bacterial cellulose produced by Acetobacter is different from the cellulose widely existing in nature, and it has the following unique properties: (1) cellulose has high purity, high crystallinity and high degree of overlap, and exists as a single fiber, so Very advantageous in the preparation of some microfiber products. The preparation of traditional microfiber products from natural fibers require...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P39/00C12P19/04C12P7/625C12R1/01
Inventor 张甜胡胜军皮埃尔鲁克·川柏雷许梦莹
Owner WUHAN UNIV OF TECH
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