Extraction method and detection method of allelochemicals in tobacco-planting soil

A technology of allelochemical substances and extraction methods, applied in the field of extraction of allelochemical substances in tobacco planting soil, can solve the problems of unspecified allelopathic autotoxicity, undeveloped detection methods, etc., and achieve the goal of solving tobacco continuous cropping obstacles, wide application and Promotion, various effects

Inactive Publication Date: 2019-05-21
HUBEI TOBACCO SCI RES INST
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Fu Jianguo et al. conducted preliminary separation and identification of acidic organic components in the rhizosphere soil of crop rotation and continuous cropping tobacco fields, but did not really clarify which components in the continuous cropping tobacco planting soil were allelochemicals, and used the area normalization method to quantify each substance , the relative content is determined, and it is not specified which substances are really allelopathic substances, and no specific detection method has been developed

Method used

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  • Extraction method and detection method of allelochemicals in tobacco-planting soil
  • Extraction method and detection method of allelochemicals in tobacco-planting soil
  • Extraction method and detection method of allelochemicals in tobacco-planting soil

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Experimental program
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Effect test

Embodiment 1

[0027] The screening of embodiment 1 extractant

[0028] Take the root soil of the tobacco plants in the tobacco planting field that has been continuously cropped for 5 years, air-dry it naturally in a cool place indoors, remove stones, plant roots and humus in the soil, and then pass it through a 2mm sieve. Weigh 25g of soil sample into a 250mL Erlenmeyer flask with a stopper, add extractant to the Erlenmeyer flask, vibrate and extract at 150rpm on a rotary oscillator for 60min, transfer the mixture in the Erlenmeyer flask to a centrifuge tube, and centrifuge at 4000 rpm Centrifuge for 5 minutes, transfer the supernatant to a round-bottomed flask, and concentrate on a rotary evaporator to obtain 20 mL of concentrated soil extract.

[0029] In order to optimize the extraction solvent, the soil was extracted with solvents No. 1-7 in Table 1 and concentrated to 20 mL, and the concentrated solution contained 1.25 g of soil extract per mL. Add 5mL of concentrated solution to ea...

Embodiment 2

[0034] Confirmation of Allelochemicals in Example 2 Tobacco Planting Soil

[0035] The first step: according to the figure 1 The extraction method shown was performed on soil samples.

[0036] Take the root soil of the tobacco plants in the tobacco planting field that has been continuously cropped for 5 years, air-dry it naturally in a cool place indoors, remove stones, plant roots and humus in the soil, and then pass it through a 2mm sieve. Weigh 25g of soil samples in a 250mL conical flask with a stopper, add methanol / water (volume ratio 5:1) extractant to the conical flask, shake and extract at 150rpm for 60min on a rotary oscillator, and put the mixture in the conical flask Transfer to a centrifuge tube, centrifuge at 4000 rpm for 5 min, transfer the supernatant to a round-bottomed flask, and concentrate on a rotary evaporator to obtain 20 mL of concentrated soil extract.

[0037] Add 2% hydrochloric acid solution dropwise to the concentrated soil extraction solution ...

Embodiment 3

[0064] Embodiment 3 The accuracy experiment of extraction method and detection method described in the present invention

[0065] Take the soil of continuous cropping for 3 years and extract according to the method of No. 9 in Table 1 of Example 1, and use the detection method in Example 2 to detect, 6 groups of parallel determinations, and the RSD values ​​of 13 kinds of allelochemicals 6 parallel determinations are between 1.25% Between -5.63% (see Table 3), it can be seen that the extraction method and detection method of the present invention have high precision and good repeatability.

[0066] Table 3: Precision of parallel determination of 13 allelochemicals after 6 groups

[0067]

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Abstract

The invention relates to an extraction method and detection method of allelochemicals in tobacco-planting soil, and the method comprises the following steps: extracting allelochemicals in the tobacco-planting soil, separating according to alkaline, neutral and acidic components, concentrating, and determining by using a gas chromatograph-mass spectrometer. The method disclosed by the invention hasthe advantages of good extraction specificity on the allelochemicals of the tobacco-planting soil, high extraction efficiency, simplicity in operation, rapidness and sensitivity in detection and thelike.

Description

technical field [0001] The invention belongs to the technical field of tobacco planting soil analysis, and in particular relates to an extraction method and a detection method of allelochemicals in tobacco planting soil. Background technique [0002] In my country, due to the constraints of arable land resources and water conservancy conditions, continuous cropping of tobacco fields is very common. However, tobacco, as a non-continuous cropping crop, often exhibits continuous cropping obstacles, such as: increased tobacco leaf diseases and insect pests, and decreased yield and quality. Many studies have shown that tobacco continuous cropping obstacles are closely related to allelopathic autotoxicity. Allelochemicals secreted by plant roots can affect tobacco plants by affecting cell membrane permeability, enzyme activity, ion absorption, water absorption, photosynthesis, protein and DNA synthesis, etc. grow. Therefore, establishing an accurate method for determining allelo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 覃光炯饶雄飞吴哲宽杨艳华兰明蓉余振
Owner HUBEI TOBACCO SCI RES INST
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