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A Method for Assessing the Toxicity of Microcystins in Real Aquatic Environments

A microcystin, water environment technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, material inspection products, etc., can solve the potential toxicity hazards of microcystin, and the research results are not convincing and other issues, to achieve the effects that are conducive to growth and survival, universal applicability, and wide application range

Active Publication Date: 2022-02-08
HEFEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] These devices and methods only detect and analyze the content of microcystins, and do not reflect the potential toxicity hazards of microcystins; and the current methods for evaluating the toxicity of microcystins are all based on a single microcystin solution. Yes, if the application of the research results in environmental water microcystin toxicity evaluation is considered, the convincing results will be less convincing because the real water environment is not restored

Method used

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  • A Method for Assessing the Toxicity of Microcystins in Real Aquatic Environments
  • A Method for Assessing the Toxicity of Microcystins in Real Aquatic Environments
  • A Method for Assessing the Toxicity of Microcystins in Real Aquatic Environments

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Embodiment 1

[0048] This embodiment includes the following steps:

[0049] (1) The degradation strain M9 before irradiation was prepared into bacterial cell suspension and diluted to 10 9 each / mL; then prepare the stock solution where the degradation strain and MC-LR coexist;

[0050] The preparation method of the stock solution in which the degrading strain and MC-LR coexist is as follows: take 200 μL of 50 μM pure MC-LR stock solution and add the bacterial cell concentration to 10 9 In 800 μL cell suspension per mL, the volume ratio of cell suspension to MC-LR solution in the prepared solution is 4:1; shake evenly, and culture at room temperature in the dark for three days; then use a filter membrane with a pore size of 0.22 μm Filter and store in a -20°C refrigerator to prepare a stock solution in which the degrading strain and MC-LR coexist.

[0051] (2) Prepare MC-LR and strain coexisting working solution with concentrations of 1 μM and 0.1 μM, pure MC-LR working solution at 1 μM an...

Embodiment 2

[0062] Degraded strains after ion beam irradiation, prepared coexisting working solution with MC-LR, infected Hep G2 cells and carried out MTT experiment, and then detected its absorbance value, compared with the degraded strains before irradiation in Example 1. The strength of the degradation effect.

[0063] This embodiment includes the following steps:

[0064] (1) by N + Ion beam implantation technology was used to irradiate the degrading strain M9 to obtain degrading strains A1, A2 and A3 after irradiation;

[0065] A1: The radiation dose is 40 units, each unit is 2.6×10 13 N + / cm 2 ,

[0066] A2: The radiation dose is 60 units, each unit is 2.6×10 13 N + / cm 2 ,

[0067] A3: The radiation dose is 80 units, each unit is 2.6×10 13 N + / cm 2 ,

[0068] (2) The degradative strain M9 before irradiation and the degradative strain A1, A2, A3 after irradiation were prepared into bacterial cell suspension, and diluted to 10 9 each / mL; then prepare respectively the ...

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Abstract

The invention discloses a method for assessing the toxicity of microcystins in real water environments. By preparing a coexistence solution of degrading bacteria and microcystin-LR (Microcystin-LR, MC-LR), constructing There are similar poisoned samples in the real water environment; then human liver cancer cells were poisoned, and the method of detecting cell metabolic activity by MTT was used to verify the toxicity of MC‑LR under the coexistence of degrading strains. The invention simulates the state of MC‑LR coexisting with degrading strains in real water environments such as lakes, makes up for the limitations of single MC‑LR toxicity research, and can better evaluate the toxicity of MC‑LR. The application prospects of industries such as aquaculture and environmental governance are better; the present invention has universal applicability to various microcystin isomers and has a wide range of applications; the present invention passes N + Ion beam implantation technology was used to irradiate the degrading strains to obtain the degrading strains after irradiation; the degradation effect on MC‑LR was compared with the degrading strains before irradiation, and then the feasibility of the method was verified.

Description

technical field [0001] The invention relates to a method for evaluating the toxicity of microcystins, in particular to a method for evaluating the toxicity of microcystins in a real water environment. Background technique [0002] Microcystins are the most widely distributed hepatotoxins, mainly produced by freshwater algae Microcystis aeruginosa, with high stability. It can strongly inhibit the activity of protein phosphatase and is also a strong liver tumor promoter. The Chinese drinking water standard limits the toxin content in drinking water to 1 μg / L. [0003] Since the 1950s, with the development of social technology and culture, due to the abuse of phosphorus-containing washing powder and pesticides, water bodies have become eutrophic, and water eutrophication can promote the growth of algae such as blue-green algae, forming a water surface. A thick layer of blue-green lake indigo with an unpleasant smell. Among the various algal toxins released by cyanobacteria a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/50C12Q1/02
Inventor 王晓飞肖厚荣张敏吴茜茜鲍腾
Owner HEFEI UNIV