Cell cryopreservation liquid

Abstract

The invention discloses a cell cryopreservation liquid, which is characterized in that the cell cryopreservation liquid comprises, by volume, the following components: 5-10 v / v% of glycerol; 15-20 v / v% of a human serum albumin solution; 10-15v / v% of a 18AA compound amino acid solution; 15-54 v / v% a dextran-40 glucose solution; 1-42.5v / v% of a mixed sugar electrolyte solution. The cell cryopreservation liquid does not contain dimethyl sulfoxide (DMSO) and has low cytotoxicity; the human serum albumin is used for replacing components such as serum, plasma and the like, thereby having higher clinical safety; the cell cryopreservation liquid can be directly prepared by using commercially available injection liquid, and has the advantages of simple and convenient operation and low cost; the additive components are clear and convenient for scientific research and analysis; the safety is high, and the damage to cells is small; the cell expansion ability is good after cryopreservation resuscitation; the cell cryopreservation liquid can be used for replacing frozen liquid containing dimethyl sulfoxide (DMSO).

Description

technical field [0001] The invention relates to the technical field of cells and bioengineering, in particular to a cell cryopreservation solution. Background technique [0002] Cell cryopreservation is one of the main methods of cell preservation. Specifically, cell cryopreservation refers to the use of cryopreservation technology to store cells at low temperature. This technology can temporarily remove cells from the growth state, thereby preserving their cell characteristics. When needed, cells can be resuscitated by cell recovery technology for experiments or other purposes. . [0003] At present, dimethyl sulfoxide and serum are usually added to the cell cryopreservation solution commonly used in laboratories or commercially available cell cryopreservation solutions. When storing cells at low temperature, dimethyl sulfoxide (DMSO) can effectively prevent cell damage caused by the formation of intracellular ice crystals, changes in osmotic pressure, and disordered cell...

AI Technical Summary

Problems solved by technology

Although it is generally believed that when the concentration is less than 10%, the toxic effect of dimethyl sulfoxide (DMSO) is very small, but in the treatment of diseases, different concentrations of dimethyl sulfoxide (DMSO) will still produce many side effects , while dimethyl sulfoxide (DMSO) has also been found to be toxic to a variety of cells, such as odontoblasts, colon tumor cells, astrocytes, stem cells, etc., which may involve multiple mechanisms, including increased membrane permeability , the diffusion of water, the reduction of intracellular lipid droplets, etc.

Serum, as a substance whose composition is not yet clear, has certain batch-to-batch differences, resulting in unstable effects of cell cryopreservation fluid, and clinical use may lead to allergies

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