Unlock instant, AI-driven research and patent intelligence for your innovation.

Preparation method of diosmin-7-o-glucuronide

A technology of glucuronide and diosmin, which is applied in the field of preparation of diosmin-7-O-glucuronide, can solve the problems that the pharmacological activity has not been reported, and achieve stable process, short steps and simple preparation process Effect

Active Publication Date: 2021-06-11
XINJIANG INST OF MATERIA MEDICA
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no commercially available pure diosmin-7-O-glucuronide, and its pharmacological activity has not been reported yet

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of diosmin-7-o-glucuronide
  • Preparation method of diosmin-7-o-glucuronide
  • Preparation method of diosmin-7-o-glucuronide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: The preparation method of this diosmin-7-O-glucuronide is carried out according to the following steps: the first step is to dry the aerial part of the required amount of sweet green orchid with a volume percentage of 30 % to 50% ethanol solution for reflux extraction for 3h to 6h to obtain the extract of Cymbidium, filter the extract for 1 to 3 times, combine the filtrates and concentrate under reduced pressure to obtain 0.8 g / mL to 1.0 g / mL The fragrant green orchid concentrated solution; the second step, the fragrant green orchid concentrated solution is used for macroporous resin chromatography, and the eluate obtained by washing the fragrant green orchid concentrated solution when the ethanol solution volume percentage is 70% is collected to obtain the first One eluent, distill the first eluent to remove ethanol in the first eluent, and dry the obtained thick extract solution at 50°C to 60°C to obtain extract powder; the third step is to extract the ex...

Embodiment 2

[0024] Embodiment 2: The preparation method of this diosmin-7-O-glucuronide is carried out according to the following steps: the first step, the dried aerial part of the required amount of sweet green orchid, with a volume percentage of 30 % or 50% ethanol solution for reflux extraction for 3h or 6h to obtain the extract of Cymbidium, filter the extract of Cymbidium 1 or 3 times, combine the filtrates and concentrate under reduced pressure to obtain 0.8 g / mL or 1.0 g / mL The fragrant green orchid concentrated solution; the second step, the fragrant green orchid concentrated solution is used for macroporous resin chromatography, and the eluate obtained by washing the fragrant green orchid concentrated solution when the ethanol solution volume percentage is 70% is collected to obtain the first One eluent, distill the first eluent to remove ethanol in the first eluent, and dry the obtained thick extract solution at 50°C or 60°C to obtain extract powder; the third step is to extract...

Embodiment 3

[0025] Embodiment 3: As the optimization of the above embodiment, in the first step, the weight of ethanol solution is 40 times to 60 times of the weight of fennel.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
melting pointaaaaaaaaaa
purityaaaaaaaaaa
Login to View More

Abstract

The invention relates to the technical field of preparation of diosmin-7-O-glucuronide, and relates to a preparation method of diosmin-7-O-glucuronide, which comprises drying the aerial part of a required amount of sweet green orchid, Reflux extraction with ethanol solution and concentration under reduced pressure, use macroporous resin chromatography to obtain eluent from the concentrated solution, distill and dry to obtain extract powder; after dissolving the extract powder with methanol solution, use Sephadex LH‑20 column Chromatography, collecting the eluent and evaporating to dryness under reduced pressure to obtain the crude product; dissolving the crude product with acetonitrile solution, using ODS column chromatography, collecting the eluent, and evaporating to dryness under reduced pressure to obtain diosmin-7-O-glucosin acid glycosides. The present invention adopts Sephadex LH-20 and ODS column chromatography combined separation method, the preparation process is simple, the steps are short, the process is stable, and the purity of the obtained diosmin-7-O-glucuronide can reach more than 98%.

Description

technical field [0001] The invention relates to the technical field of diosmin-7-O-glucuronide preparation, and relates to a preparation method of diosmin-7-O-glucuronide. Background technique [0002] Xiangqinglan is a real estate ethnic medicine in Xinjiang. Preliminary pharmacodynamic studies have shown that its effective parts have pharmacological effects such as anti-myocardial ischemia and atherosclerosis. Dessert-7-O-glucuronide is one of the main components in the effective fraction of Cinnamon Orchid. Digerin-7-O-glucuronide is a chemical substance whose structural formula is figure 1 shown. There is no commercially available pure diosmin-7-O-glucuronide, and its pharmacological activity has not been reported yet. Contents of the invention [0003] The present invention provides a preparation method of diosmin-7-O-glucuronide, which overcomes the above-mentioned deficiencies in the prior art, and can extract and separate high-purity diosminus from chrysanthemum...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/07C07H1/08
Inventor 邢建国谭梅娥何承辉于宁郑瑞芳王云飞
Owner XINJIANG INST OF MATERIA MEDICA