Method for improving yield of allocholic acid from duck bile

A technology for the identification of cholic acid and duck bile, which is applied in the field of bioengineering, can solve the problems of environmental protection risks, high control pressure, high equipment resource occupancy, and large solvent consumption, and achieve rapid industrialization, optimization of production processes, solvents, etc. costly effect

Active Publication Date: 2019-05-28
CHANGDE YUNGANG BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to make the extraction purity and yield of allocholic acid higher, people have adopted a variety of extraction methods, such as first separating chenodeoxycholic acid and other substances in duck bile, but the effect is not significant, because the separation of goose When oxycholic acid and other substances are removed, not only the purity of chenodeoxycholic acid will be reduced, but also allocholic acid will be lost, which will definitely affect the extraction yield of allocholic acid
In addition, the current extraction method of allocholic acid generally adopts the traditional magnesium salt method. Due to the large volume of allocholic acid intermediates produced by the magnesium salt method, the high occupancy rate of equipment resources, large solvent consumption, high safety risks, and environmental protection The risk and control pressure are very high, which is not conducive to industrial mass production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A method that can improve the yield of allocholic acid from duck bile, comprising the steps of:

[0032] (1) Preparation of extract: take 500 kg of duck bile raw material, saponify to obtain saponified liquid, add sodium hydroxide to the saponified liquid to make the mass percentage concentration of sodium hydroxide reach 14%, cool at room temperature until the precipitate floats in the saponified liquid On the surface, separate and collect the saponified liquid in the lower layer to obtain saponified precipitates. Add 75 kilograms of water in the saponification precipitate, after heating and dissolving, add content while stirring and be 20% dilute hydrochloric acid, adjust pH value to be 7.5, add the mixed solvent of 350 kilograms of butyl acetate, ethylene dichloride and butanol, The ratio of butyl acetate, dichloroethane and butanol is 8:1:1, then the temperature is controlled at 54°C, and dilute hydrochloric acid with a content of 8.5% is added while stirring, and t...

Embodiment 2

[0041] A method that can improve the yield of allocholic acid from duck bile, comprising the steps of:

[0042] (1) Preparation of extract: take 520 kg of duck bile raw material, saponify to obtain saponification liquid, add sodium hydroxide to the saponification liquid to make the mass percentage concentration of sodium hydroxide reach 14.5%, cool at room temperature until the precipitate floats in the saponification liquid On the surface, separate and collect the saponified liquid in the lower layer to obtain saponified precipitates. Add 78 kilograms of water in the saponification precipitate, after heating and dissolving, add content while stirring and be 20% dilute hydrochloric acid, adjust pH value to be 7.5, add the mixed solvent of 360 kilograms of butyl acetate, ethylene dichloride and butanol, The ratio of butyl acetate, dichloroethane and butanol is 8:1:1, then the temperature is controlled at 53.5°C, and dilute hydrochloric acid with a content of 8% is added while s...

Embodiment 3

[0051] A method that can improve the yield of allocholic acid from duck bile, comprising the steps of:

[0052](1) Preparation of extract: take 480 kg of duck bile raw material, saponify to obtain saponification liquid, add sodium hydroxide to the saponification liquid to make the mass percentage concentration of sodium hydroxide reach 14.2%, cool at room temperature until the precipitate floats in the saponification liquid On the surface, separate and collect the saponified liquid in the lower layer to obtain saponified precipitates. Add 72 kilograms of water in the saponification precipitate, after heating and dissolving, add content while stirring and be 20% dilute hydrochloric acid, adjust pH value to be 7.6, add the mixed solvent of 340 kilograms of butyl acetate, ethylene dichloride and butanol, The ratio of butyl acetate, dichloroethane and butanol is 8:1:1, then the temperature is controlled at 54.5°C, and dilute hydrochloric acid with a content of 7.5% is added while ...

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Abstract

The invention discloses a method for improving the yield of allocholic acid from duck bile, comprising the following process steps such as preparation of extract liquor, preparation of chenodeoxycholic acid and allocholic acid amine salt, refining of amine salt, preparation of allocholic acid, vacuum recovery of a chenodeoxycholic acid solution, and the like. By adopting the method instead of a magnesium salt method, a production process of extracting allocholic acid is optimized, the industrialization can be realized quickly, and the problem of environmental protection pressure under the national environmental protection high pressure supervision situation is solved.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a method capable of increasing the yield of allocholic acid from duck bile. Background technique [0002] In the field of biology, the development and utilization of bile acids is one of the important subjects of people's research, especially driven by the daily development of biochemistry, the research on bile acids is constantly deepening. However, bile acid resources are limited in the biological world, so any kind of bile acid is important. The main bile acids in duck bile are allocholic acid and chenodeoxycholic acid, wherein the content of allocholic acid is 0.9%-1.1%, and the content of chenodeoxycholic acid is 1.0%-1.2%. Because allocholic acid is an excellent feed additive ingredient and an important resource for synthesizing cholic acid, chenodeoxycholic acid and other bile acids, its research, development and utilization are also expanding to new fields. In or...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J9/00
Inventor 邓家国雷平蔡宏军
Owner CHANGDE YUNGANG BIOTECHNOLOGY CO LTD
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