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A polysaccharide-producing endogenous Anthrocystus ut-x strain and its application

A technology of UT-X and charcoal horn bacteria, which is applied in the field of microorganisms, can solve the problems of less endophytic bacteria in Ulva, and achieve the effects of good antioxidant activity, low fermentation cost and high economic benefit.

Inactive Publication Date: 2020-10-16
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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Problems solved by technology

[0004] The endophytes of Brasenia have only begun to attract people's attention in recent years. Generally speaking, there are relatively few studies on the endophytes of Brasenia, especially those that can metabolize the corresponding polysaccharide components of Brasenia, and there are few studies on the endophytes of Brasenia that produce polysaccharides. report

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  • A polysaccharide-producing endogenous Anthrocystus ut-x strain and its application
  • A polysaccharide-producing endogenous Anthrocystus ut-x strain and its application
  • A polysaccharide-producing endogenous Anthrocystus ut-x strain and its application

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Embodiment Construction

[0014] The present invention will be described in detail below in conjunction with specific examples, but the protection scope of the present invention is not limited to the following examples.

[0015] Isolation of Endophytic Bacteria from Brasenia officinalis

[0016] Take the stems, leaves and roots of fresh water shield, sterilize them with mercuric chloride and rinse them with distilled water several times in a sterile environment, inoculate them on LB solid medium, and culture them in the dark. Well-growing colonies were picked from the culture medium and transferred to new LB medium plates, purified and numbered respectively. The bacterial strain provided in the present embodiment is named UT-X bacterial strain, and the bacterium colony of this bacterial strain is moved to the new LB medium plate and grows well, and its bacterium colony morphology figure is as follows figure 1 as shown, figure 1 Middle scale bar = 2mm. From figure 1 It can be seen that the diameter ...

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Abstract

The invention provides a polysaccharide producing endogenous xylaria UT-X strain and belongs to an endogenous xylariaceae sp. strain. The strain is preserved in China Center for Type Culture Collection, the address is Wuhan University Wuhan China, the postcode is 430072, the preservation date is January 17th 2019, a preservation number is CCTCC M 2019055, and a 16S rDNA sequence of the strain is shown as SEQ ID NO.1. The endogenous xylaria UT-X strain can be applied to fermentation to prepare polysaccharide. The strain disclosed by the invention can perform large-scale fermentation culture ina short time; furthermore, fermentation cost is lower, and limits of conditions of areas, seasons and the like are avoided; thus, the current situation that water shield natural resources are increasingly scarce can be solved through a microbial fermentation way, so that higher economical benefits are achieved.

Description

technical field [0001] The invention relates to an endophytic Anthracanthus strain, which can produce polysaccharides and belongs to the technical field of microbes. Background technique [0002] At present, the research on plant endophytes has been paid more and more attention, and various endophytes of different plants have been discovered one after another. The diversity of endophyte species also endows the diversity of its metabolites, which also indicates that the use of microbial metabolism to discover new resources such as biology, chemistry, and medicine has great potential. A large number of studies have shown that plant endophytes can produce the same or similar active substances and precursors as those of host plants. This discovery directly promotes the extensive research on plant endophytes, especially economical plant endophytes. With people's gradual research on economical plant endophytes, the use of endophytic bacteria that can metabolize and produce corres...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P19/04C12R1/645
Inventor 余光辉岳涵覃瑞龚汉雨
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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