Multi-gene detection kit for anti-depression drug medication guidance and using method of kit

Abstract

The invention discloses a multi-gene detection kit for anti-depression drug medication guidance and a using method of the kit. A multi-PCR and fragment length / mass analysis method is used for synchronously, quickly and quantitatively detecting seven single nucleotide polymorphism (SNP) loci on four genes, including CYP2D6, CYP2C19, ABCB1 and FKBP5, related to anti-depression drug metabolism, transfer and target site actions. Detection includes the steps that 1, oral cast-off cells are collected and stored in a cell acquisition card or a blood sample is collected and nucleic acid is extracted;2, the cell acquisition card or the nucleic acid in step 1 serves as a template for multi-PCR amplification; 3, according to the length / mass of PCR product fragments, the seven SNP loci, three human DNA referenc genes and a PCR product of a PCR reaction internal reference are synchronously separated; 4, results are subjected to analysis and interpretation. The kit has the advantages of being quickto use, high in sensitivity, good in repeatability, high in specificity, high in flux and low in cost.

Description

Technical field [0001] The invention relates to a multiple gene detection kit, in particular to a multiple gene detection kit used for guiding antidepressant medication and a method of use thereof. Background technique [0002] Depression is a common affective disorder that can suffer for life. It occurs at various ages and is characterized by repeated attacks and intermittent periods of intact state. According to the data released by the World Health Organization (WHO), the incidence of depression is increasing year by year. There are more than 300 million people suffering from depression worldwide, of which 89 million suffer from severe depression. Around 800,000 people commit suicide due to depression every year in the world. Suicide has become the second cause of death in the 15-29 year old age group. The WHO health report in 2001 pointed out that depression accounted for the largest proportion of the global disease burden (11.9%), far exceeding chronic diseases such as diab...

AI Technical Summary

Problems solved by technology

However, the Sanger sequencing method is complex and expensive

When there are many sequencing sites, it needs to be sequenced one by one, which takes a long time and the cumulative price is relatively expensive

The next-generation sequencing technology realizes sequencing while synthesizing, which has the advantages of high throughput and high efficiency. However, the next-generation sequencing platform is expensive and has low popularity, and its clinical application as a SNP detection technology is not mature enough.

[0015] Due to the large number of gene SNPs related to antidepressant medication guidance, the above techniques have obvious limitations, so it is difficult to apply multiple gene detection for antidepressant medication guidance

[0016] At present, there are no reports in China on the multiplex gene detection scheme for antidepressant medication guidance based on multiplex PCR and CE separation technology

Images