Multi-gene detection kit for anti-depression drug medication guidance and using method of kit

A technique of medication guidance and genetic testing, applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems of time-consuming, expensive accumulation, high cost, etc., to avoid false negatives and false positives, and have good repeatability , strong specific effect

Inactive Publication Date: 2019-05-31
NINGBO HEALTH GENE TECHNOLOGIES CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the Sanger sequencing method is complex and expensive
When there are many sequencing sites, it needs to be sequenced one by one, which takes a long time and the cumulative price is relatively expensive
The next-generation sequencing technology realizes sequencing while synthesizing, which has the advantages of high throughput and high efficiency. However, the next-generation sequencing platform is expensive and has low popularity, and its clinical appli

Method used

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  • Multi-gene detection kit for anti-depression drug medication guidance and using method of kit
  • Multi-gene detection kit for anti-depression drug medication guidance and using method of kit
  • Multi-gene detection kit for anti-depression drug medication guidance and using method of kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] In this example, the oral exfoliated cell collection card was used as a template to directly perform multiple PCR reactions, and finally the samples were separated by capillary electrophoresis. The specific steps are as follows:

[0048] 1. Production of multiple genetic testing kits for the guidance of antidepressant medications, including the following components:

[0049] 1) MDD Primer Mix;

[0050] 2) PCR reaction solution;

[0051] 3) Positive control substance MDD POS;

[0052] 4) Ultra-pure water.

[0053] 2. Collect samples

[0054] Use a buccal swab to collect the oral exfoliated cells of a depressed patient and save them on a cell collection card.

[0055] 3. Carry out PCR reaction with cell acquisition card as template

[0056] 1) Add reagents and samples to the 96-well sample plate / PCR eight-strip tube according to Table 4.

[0057] Table 4 PCR reaction system

[0058] Reagent

Quantity (μL) / well

PCR reaction solution

14

Primer composition

2

Cell Collection Card

0

...

Embodiment 2

[0078] In this embodiment, a whole blood sample of a depression patient is collected and nucleic acid is extracted. The extracted nucleic acid is used as a template to perform a multiplex PCR reaction, and finally the sample is separated by capillary electrophoresis. The specific steps are as follows:

[0079] 1. Production of multiple gene detection kits for the guidance of antidepressant medications. The components of the kit are as described in Example 1.

[0080] 2. Collect samples

[0081] Collect a whole blood sample from a depression patient and extract nucleic acid.

[0082] 3. Use the extracted nucleic acid as a template for PCR reaction

[0083] 1) Add reagents and samples to the 96-well sample plate / PCR eight-strip tube according to Table 9.

[0084] Table 9 PCR reaction system

[0085]

[0086]

[0087] 2) Mix and centrifuge the prepared PCR system to perform PCR reaction. The PCR procedure is as described in Example 1.

[0088] 3) Capillary electrophoresis to separate samples,...

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PUM

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Abstract

The invention discloses a multi-gene detection kit for anti-depression drug medication guidance and a using method of the kit. A multi-PCR and fragment length/mass analysis method is used for synchronously, quickly and quantitatively detecting seven single nucleotide polymorphism (SNP) loci on four genes, including CYP2D6, CYP2C19, ABCB1 and FKBP5, related to anti-depression drug metabolism, transfer and target site actions. Detection includes the steps that 1, oral cast-off cells are collected and stored in a cell acquisition card or a blood sample is collected and nucleic acid is extracted;2, the cell acquisition card or the nucleic acid in step 1 serves as a template for multi-PCR amplification; 3, according to the length/mass of PCR product fragments, the seven SNP loci, three human DNA referenc genes and a PCR product of a PCR reaction internal reference are synchronously separated; 4, results are subjected to analysis and interpretation. The kit has the advantages of being quickto use, high in sensitivity, good in repeatability, high in specificity, high in flux and low in cost.

Description

Technical field [0001] The invention relates to a multiple gene detection kit, in particular to a multiple gene detection kit used for guiding antidepressant medication and a method of use thereof. Background technique [0002] Depression is a common affective disorder that can suffer for life. It occurs at various ages and is characterized by repeated attacks and intermittent periods of intact state. According to the data released by the World Health Organization (WHO), the incidence of depression is increasing year by year. There are more than 300 million people suffering from depression worldwide, of which 89 million suffer from severe depression. Around 800,000 people commit suicide due to depression every year in the world. Suicide has become the second cause of death in the 15-29 year old age group. The WHO health report in 2001 pointed out that depression accounted for the largest proportion of the global disease burden (11.9%), far exceeding chronic diseases such as diab...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883
Inventor 吕军英张成王凡吴勇余丁
Owner NINGBO HEALTH GENE TECHNOLOGIES CO LTD
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