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Paper-based microfluidic chip used for detecting glucose concentration and detection method

A microfluidic chip, glucose concentration technology, applied in chemical instruments and methods, laboratory containers, material analysis through observation of the impact on chemical indicators, etc., can solve the problem of slow analysis speed, high cost, real-time on-site Difficult to detect and other problems, to achieve the effect of simple operation, convenient operation and low production cost

Inactive Publication Date: 2019-06-04
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the determination methods of glucose mainly include spectrophotometry, electrochemical method and high performance liquid chromatography, etc., but these technologies have certain limitations such as slow analysis speed, high cost, and difficulty in on-site instant detection.

Method used

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  • Paper-based microfluidic chip used for detecting glucose concentration and detection method
  • Paper-based microfluidic chip used for detecting glucose concentration and detection method
  • Paper-based microfluidic chip used for detecting glucose concentration and detection method

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Experimental program
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Effect test

Embodiment 1

[0034] The method for detecting glucose concentration using the paper-based microfluidic chip with the length change of the color band according to the present invention comprises the following steps:

[0035] 1) Preparation of titanium dioxide nanoparticle suspension: 0.1 g of titanium dioxide powder with a particle size of about 25 nm was dispersed in 10 mL of ethanol, and ultrasonicated for 1 hour to make it uniformly dispersed. 20 μL of TMOS (trimethoxyoctadecylsilane, octadecyltrimethoxysilane) was added to the dispersion liquid, after ultrasonication for 10 minutes, and reacted at room temperature for 12 hours, the silanized titanium dioxide nanoparticle suspension was obtained.

[0036] 2) Preparation of ultra-hydrophobic filter paper with micro-nano composite structure: cut the filter paper into small pieces of 1.5cm×3cm, then soak the filter paper in the silane-treated dispersion, take it out after 5 minutes, and dry it naturally at room temperature, and repeat the ope...

Embodiment 2

[0047] Use a dropper to draw 2 μL of 2.5mM, 5mM, 10mM, 15mM, 20mM, 25mM, 30mM, 40mM, 45mM, 50mM glucose solutions, and drop them on the flow channel of the paper-based microfluidic chip. Obviously, different concentrations of glucose solutions correspond to different lengths of color bands. The length of glucose solutions with different concentrations flowing on the flow channel can be easily measured with a steel ruler. According to this method, a standard library with one-to-one correspondence between the flow length and the glucose concentration can be established, so as to detect the concentration of the unknown glucose solution.

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Abstract

The invention provides a paper-based microfluidic chip used for detecting glucose concentration based on length changes in a color band. The difference in the length of the color bands formed by glucose solutions of different concentrations in the detection flow path of the self-driving paper-based microfluidic chip in wetting gradient formed by strip-shaped gradient arrays and wedge channels is used to establish a standard library in which glucose concentration and the length of color bands thereof flowing on the self-driving paper-based microfluidic chip with the wetting gradient formed by the strip-shaped gradient arrays and the wedge channels are one-to-one corresponding. When glucose concentration is measured, a glucose solution to be detected is aspirated with a dropper. The length of the color band formed by the glucose solution in the detection flow path is measured and compared with the length of the color bands in the established glucose standard library. The length of the color band of glucose concentration of the same length is matched. The concentration of the glucose solution to be detected can be detected. The method has the advantages of simple operation and being convenient and economical, and can accurately detect the concentration of the glucose solution.

Description

technical field [0001] The invention relates to a paper-based microfluidic chip and a detection method for glucose concentration detection. Background technique [0002] According to the survey, the incidence of diabetes in China has increased sharply in the past 30 years, and there are currently 264 million people with abnormal blood sugar. Glucose is an indispensable substance in human life activities, and its content is an important indicator to measure metabolic capacity and monitor disease conditions. Especially in the diagnosis of clinical diabetes, real-time monitoring of blood glucose concentration is an important means to control the patient's condition. Currently, glucose determination methods mainly include spectrophotometry, electrochemical method, and high-performance liquid chromatography, etc., but these techniques have certain limitations such as slow analysis speed, high cost, and difficulty in on-site instant detection. Therefore, the establishment of a fa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78B01L3/00
Inventor 吴化平刘其辉朱凯彭翔张征丁浩
Owner ZHEJIANG UNIV OF TECH
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