Lactobacillus-containing chocolate and manufacturing method therefor
A technology of chocolate and Lactobacillus brevis, applied in the direction of lactobacillus, bacteria used in food preparation, cocoa, etc., can solve problems such as research on solid particle size
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Embodiment 1
[0054] 1. Preparation of Lactobacillus Powder
[0055] Lactic acid bacterium brevis subsp. coagulans (Labre bacteria) was used as the lactic acid bacteria powder. Labre bacteria are cultured using a common lactic acid bacteria medium (MRS medium, etc.), and the cultured bacteria are collected by centrifugation, etc., freeze-dried, powdered, etc. to obtain a powder, and starch is added thereto to obtain a lactic acid bacteria powder.
[0056] 2. Preparation of chocolate containing lactic acid bacteria
[0057] Chocolate mass 1-4 containing sugar, cocoa mass, cocoa butter, whole milk powder or milk, emulsifier, flavoring, etc. were prepared. Chocolates 1-4 having a high concentration of lactic acid bacteria were produced by melting each chocolate mass at 40°C and adding a high concentration of lactic acid bacteria powder thereto. Each chocolate mass 1-4 after tempering was mixed with a corresponding chocolate 1-4 containing a high concentration of lactic acid bacteria, cooled,...
Embodiment 2
[0072] Study on Particle Size Distribution of Solid Particles in Chocolate Containing Lactic Acid Bacteria
[0073] The particle size distribution of the solid particles in chocolate 1 containing lactic acid bacteria produced in Example 1 is shown in figure 1 middle. figure 1 The particle size distribution of shows a single peak.
[0074] The relationship between the particle size distribution and water activity of solid particles in chocolate mass was investigated. Four types of chocolate mass (chocolate mass 1-4) with different particle size distributions were prepared. The particle size distribution of the solid particles in each billet is as in figure 2 shown in . These chocolate bars were stored at 25°C, 75% RH (relative humidity) for 98 hours and the time course increase in water activity over that time was observed. The time-course ERH change (%) results are shown in image 3 middle. Chocolate mass 1, 2 showed a single distribution peak and 3, 4 showed two peaks...
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