Florists chrysanthemum flower tissue culture method and tissue culture medium
A technology of tissue culture and culture medium, applied in the field of plant tissue culture, can solve the problems of not being able to meet the market demand, increase the cost of culture, and the height of tissue culture seedlings, so as to inhibit the browning of chrysanthemums, reduce the cost of reproduction, and be easy to operate Effect
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[0011] 1. Screening test of proliferation medium
[0012] The test material is the chrysanthemum HH-62 variety with rootless tissue culture seedlings. The robust tissue culture seedlings are rooted and divided into about 2.5cm in stem height and stem segments with 1-2 leaves. They are divided into 15 groups, 36 in each group. The test objects were inoculated in the proliferation medium listed in Table 1 for cultivation. The uniform light culture conditions were: temperature 25±2°C, light time 14h, light intensity 2000-3000LX, and culture days 15d.
[0013] Proliferation medium is MS medium of 0.2-1.0mg / L 6-BA+0.1-0.5mg / L NAA+30g / L sucrose+6g / L agar powder, pH is 5.8.
[0014] Table 1 Chrysanthemum Proliferation Medium Treatment Design
[0015]
[0016] 2. Screening test results of proliferation medium
[0017] Table 2 Proliferation and culture results
[0018]
[0019] It can be seen that the proliferation coefficients of treatments 12 and 13 are relatively prominent,...
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