Florists chrysanthemum flower tissue culture method and tissue culture medium

Abstract

The invention discloses a florists chrysanthemum flower tissue culture method and a tissue culture medium, and belongs to the technical field of plant tissue culture. The tissue culture medium comprises a proliferation medium and a rooting medium, wherein the proliferation medium is an MS medium with 0.8mg / L 6-BA (benzyl aminopurine), 0.5mg / L NAA (naphthylacetic acid), 0.2mg / L PVP (polyvinyl pyrrolidone), 30g / L sucrose and 6g / L agar powder, the pH (potential of hydrogen) of the proliferation medium is 5.8, and the rooting medium is a 1 / 2 MS medium with 0.6mg / L NAA, 0.2mg / L PVP, 30g / L sucrose and 6g / L agar powder, and the pH of the rooting medium is 5.8. The tissue culture method has the advantages of simplicity in operation, low cost, high florists chrysanthemum flower tissue culture seedling breeding rate and the like.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a chrysanthemum tissue culture method and a culture medium for tissue culture. Background technique [0002] Chrysanthemum (Dendranthema morifolium (Ramat.) Tzvel.) is a perennial herbaceous plant belonging to the family Asteraceae. Chrysanthemum is one of China's top ten famous flowers and one of the world's four largest cut flowers, distributed all over the world. In recent years, with the development of the world's flower industry, traditional breeding methods have been unable to meet market demand, and genetic transformation technology has shown unique advantages, which can be directional modified certain target shapes of chrysanthemums, so that it can be improved in flower color, flowering period , Stress resistance and other aspects play an important role, and the successful gene firstly depends on the establishment of a good chrysanthemum regenera...

AI Technical Summary

Problems solved by technology

In recent years, with the development of the world's flower industry, traditional breeding methods have been unable to meet market demand, and genetic transformation technology has shown unique advantages, which can be directional modified certain target shapes of chrysanthemums, so that it can be improved in flower color, flowering period , stress resistance and other aspects play an important role, and the successful gene first depends on the establishment of a good chrysanthemum regeneration system, which requires a certain regeneration frequency and transformation rate, and also needs to have stability and repeatability

Tissue culture not only has a high reproduction coefficient and a fast reproduction speed, but also can be used for detoxification and rejuvenation of varieties, preservation of germplasm resources, etc. The plant height of the tissue cultured seedlings cultured in the proliferation medium is relatively low, and they need to pass through the strong seedling medium before they can be inoculated into the rooting medium, which prolongs the cultivation time and increases the cultivation cost to a certain extent.

Images