Prepared cibotium barometz tuber effective parts and preparation method thereof
An effective part and dog ridge-making technology, applied in the field of medicine, can solve the problems of lack of action mechanism, unclear contents of various components, insufficient research on the basis of drug efficacy, etc., and achieves a simple, environmentally friendly preparation method and good safety. Effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
experiment example 1
[0039] Experimental example 1: investigation experiment of macroporous resin
[0040] (1) Preparation and content determination of the crude extract of dog ji Filtrate, the filtrate is used as the need testing solution of HPLC (high performance liquid chromatography) content determination, according to the high performance liquid chromatography of " Chinese Pharmacopoeia " 2015 edition general rules 0512 Determination of dog ridge extract content.
[0041] Chromatographic conditions and system suitability test:
[0042] Octadecylsilane bonded silica gel is used as filler; methanol is used as mobile phase A, 0.1% formic acid-ammonium formate solution is used as mobile phase B, and gradient elution method is adopted: 0min→33min→45min→70min; A: 4%→4%→95%→4%, detection by wavelength switching: detect kojic acid, protocatechuic acid and protocatechuic aldehyde at 280nm wavelength; detect 5-HMF at 260nm wavelength; column temperature 35°C; The number of theoretical boards should n...
Embodiment 1
[0065] (1) Take 40g of the medicinal material fine powder of dog spines, add 800mL of methanol-water with a volume ratio of 70:30, and extract by reflux twice, each time for 2h, filter, and adjust the pH of the filtrate to 4 with 1mol / L hydrochloric acid solution to obtain dog ridge extract;
[0066] (2) Take another 10g of DM2 resin, add 30mL of 95% ethanol to pass through the resin at a flow rate of 20mL / h and soak for 24h to make it fully swell; add 30mL of 5% hydrochloric acid solution to pass through the resin at a flow rate of 20mL / h and soak for 3h, use Wash with water until the pH is neutral, add 30mL of 2% sodium hydroxide solution, pass through the resin at a flow rate of 20mL / h and soak for 3h, wash with water until the pH is neutral, and dry at 25°C;
[0067] (3) Weigh 3 g of the DM2 type resin treated in step (2) and pack it into a column; take 15 mL of the dog ridge extract prepared in step (1) to load the sample, and after 4 hours of adsorption, elute with 45 mL...
Embodiment 2
[0076] (1) Take 40 g of the fine powder of the medicinal material of dog's spine, add 800 mL of methanol-water with a volume ratio of 70:30 and divide it into two reflux extractions, each time for 2 hours, filter, and the filtrate is set aside to obtain the dog's spine extract;
[0077](2) Take another 10g of DM2 resin, add 30mL of 95% ethanol to pass through the resin at a flow rate of 25mL / h and soak for 24h to make it fully swell; add 30mL of 5% hydrochloric acid solution to pass through the resin at a flow rate of 22mL / h and soak for 3h, use Wash with water until the pH is neutral, add 30mL of 2% sodium hydroxide solution, pass through the resin at a flow rate of 20mL / h and soak for 3h, wash with water until the pH is neutral, and dry at 25°C;
[0078] (3) Weigh 3 g of the DM2 type resin treated in step (2) and pack it into a column; take 15 mL of the dog ridge extract prepared in step (1) and put it on the sample, after absorbing for 4 hours, use 45 mL of methanol-water so...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com