Prepared cibotium barometz tuber effective parts and preparation method thereof

An effective part and dog ridge-making technology, applied in the field of medicine, can solve the problems of lack of action mechanism, unclear contents of various components, insufficient research on the basis of drug efficacy, etc., and achieves a simple, environmentally friendly preparation method and good safety. Effect

Inactive Publication Date: 2019-06-07
SHANDONG UNIV
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  • Abstract
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  • Claims
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Problems solved by technology

[0005] In recent years, the research on dog spine has gradually increased. Pharmacological studies have shown that dog spine and its extraction parts have a variety of biological activities, mainly including prevention and treatment of osteoporosis, inhibition of platelet aggregation, hemostasis and analgesia, antibacterial, anti-inflammatory, anti-rheumatism , liver protection, anti-oxidation and anti-cancer effects, but there is still a lack of research on the mechanism of action, and the research on the basis of its pharmacodynamics is not deep enough, and the content of various components is not clear
So far, there are no research reports on the extraction and preparation of protocatechuic acid, protocatechualdehyde, 5-HMF (5-hydroxymethylfurfural) and kojic acid, which are the effective parts of dog spines

Method used

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  • Prepared cibotium barometz tuber effective parts and preparation method thereof
  • Prepared cibotium barometz tuber effective parts and preparation method thereof
  • Prepared cibotium barometz tuber effective parts and preparation method thereof

Examples

Experimental program
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Effect test

experiment example 1

[0039] Experimental example 1: investigation experiment of macroporous resin

[0040] (1) Preparation and content determination of the crude extract of dog ji Filtrate, the filtrate is used as the need testing solution of HPLC (high performance liquid chromatography) content determination, according to the high performance liquid chromatography of " Chinese Pharmacopoeia " 2015 edition general rules 0512 Determination of dog ridge extract content.

[0041] Chromatographic conditions and system suitability test:

[0042] Octadecylsilane bonded silica gel is used as filler; methanol is used as mobile phase A, 0.1% formic acid-ammonium formate solution is used as mobile phase B, and gradient elution method is adopted: 0min→33min→45min→70min; A: 4%→4%→95%→4%, detection by wavelength switching: detect kojic acid, protocatechuic acid and protocatechuic aldehyde at 280nm wavelength; detect 5-HMF at 260nm wavelength; column temperature 35°C; The number of theoretical boards should n...

Embodiment 1

[0065] (1) Take 40g of the medicinal material fine powder of dog spines, add 800mL of methanol-water with a volume ratio of 70:30, and extract by reflux twice, each time for 2h, filter, and adjust the pH of the filtrate to 4 with 1mol / L hydrochloric acid solution to obtain dog ridge extract;

[0066] (2) Take another 10g of DM2 resin, add 30mL of 95% ethanol to pass through the resin at a flow rate of 20mL / h and soak for 24h to make it fully swell; add 30mL of 5% hydrochloric acid solution to pass through the resin at a flow rate of 20mL / h and soak for 3h, use Wash with water until the pH is neutral, add 30mL of 2% sodium hydroxide solution, pass through the resin at a flow rate of 20mL / h and soak for 3h, wash with water until the pH is neutral, and dry at 25°C;

[0067] (3) Weigh 3 g of the DM2 type resin treated in step (2) and pack it into a column; take 15 mL of the dog ridge extract prepared in step (1) to load the sample, and after 4 hours of adsorption, elute with 45 mL...

Embodiment 2

[0076] (1) Take 40 g of the fine powder of the medicinal material of dog's spine, add 800 mL of methanol-water with a volume ratio of 70:30 and divide it into two reflux extractions, each time for 2 hours, filter, and the filtrate is set aside to obtain the dog's spine extract;

[0077](2) Take another 10g of DM2 resin, add 30mL of 95% ethanol to pass through the resin at a flow rate of 25mL / h and soak for 24h to make it fully swell; add 30mL of 5% hydrochloric acid solution to pass through the resin at a flow rate of 22mL / h and soak for 3h, use Wash with water until the pH is neutral, add 30mL of 2% sodium hydroxide solution, pass through the resin at a flow rate of 20mL / h and soak for 3h, wash with water until the pH is neutral, and dry at 25°C;

[0078] (3) Weigh 3 g of the DM2 type resin treated in step (2) and pack it into a column; take 15 mL of the dog ridge extract prepared in step (1) and put it on the sample, after absorbing for 4 hours, use 45 mL of methanol-water so...

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Abstract

The invention provides prepared cibotium barometz tuber effective parts and a preparation method thereof. The preparation method comprises the following steps of taking 40g of prepared cibotium barometz tuber medicinal material fine powder, adding 800mL of methanol-water in the volume ratio being 70 to 30, performing reflux extraction twice, wherein the reflux extraction time each time is 2h, performing filtration, and taking filtrate for standby application; additionally taking 3g of activated DM2 type resins, and performing loading into a column; taking 15mL of a cibotium barometz tuber extracting solution, conveying samples to a detection instrument, performing adsorption for 4h, performing eluting with 45mL of methanol-water in the volume ratio being 20 to 80, collecting an eluent, andperforming evaporation to dryness so as to obtain the prepared cibotium barometz tuber effective parts. According to the cibotium barometz tuber effective parts prepared by the method, the kojic acidcontent in percentage by mass is more than 0.4%, the 5-HMF content in percentage by mass is more than 13.5%, the protocatechuic acid content in percentage by mass is more than 0.6%, and the protocatechuic aldehyde content in percentage by mass is more than 0.5%. The preparation method is simple and environmental-friendly and is suitable for large-scale industrial production.

Description

technical field [0001] The invention relates to an effective part for making dog spines and a preparation method thereof, belonging to the technical field of medicine. Background technique [0002] Dog spine, the name of Chinese medicine. It is the dried rhizome of Cibotium barometz (L.) J.Sm. Excavated in autumn and winter, remove the silt, and dry; or remove hard roots, petioles, and golden fluff, cut into thick slices, and dry to become "raw dog ridge slices"; steam and dry until 60% to 70% dry, and cut into thick slices Slices, dried, are "cooked dog ridge slices". "Chinese Pharmacopoeia" over the years has recorded it as a commonly used traditional Chinese medicine, which has the effects of dispelling rheumatism, nourishing the liver and kidney, and strengthening the waist and knees. Aggregation, anti-inflammatory, anti-rheumatic, liver protection, anti-oxidation and anti-cancer pharmacological activities. Dog ridge is mainly distributed in Zhejiang, Jiangxi, Fujian...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/12A61P19/10
Inventor 王姝麒石悦姚瑶郭常川陈亚囡
Owner SHANDONG UNIV
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