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Gene chip with high-flux detection

A gene chip and high-throughput technology, applied in chemical libraries, combinatorial chemistry, organic compound libraries, etc., can solve problems such as easy interference detection signals, blurred hybridization signals, and reduced signal-to-noise ratio, so as to improve hybridization efficiency and accuracy Improvement of performance and accuracy

Pending Publication Date: 2019-06-07
江苏苏博生物医学科技南京有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the distance between two adjacent probes of the matrix array is too close, the distance between the light-transmitting holes of the light-shielding film is very small, and there will be light diffraction problems, which will cause the final hybridization signal to be blurred and the signal-to-noise ratio to be reduced.
At the same time, the matrix array will make it difficult to wash off after hybridization and easily interfere with the detection signal

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A gene chip for high-throughput detection, including a solid-phase carrier layer and 6 gene chip areas arranged on the solid-phase carrier layer. The gene chip area is provided with a probe array, and the probe array is an array of concentric circles, forming a concentric array. The probe points on two adjacent rings of the circular array are arranged alternately.

[0032] The probe array on the above-mentioned gene chip is a concentric circle array, and the probe points of two adjacent circles are arranged in a staggered manner, which can avoid mutual interference between two adjacent points during the probe synthesis process and affect the final signal recognition , thus significantly improving the accuracy of genetic testing.

Embodiment 2

[0034] It is basically the same as in Example 1, except that the gene chip area includes a basement membrane layer and a carrying layer connected up and down, wherein the carrying layer is a nitrocellulose layer, the basement membrane layer is connected to the solid phase carrier layer, and the probe The array is set on the bearing layer, two adjacent rings forming a concentric array on the bearing layer, the height of the inner ring is higher than the height of the outer ring, and any two adjacent rings forming a concentric array on the bearing layer The height difference is equal, and the height difference between the innermost ring and the outermost ring forming the concentric circle array on the bearing layer is 1 / 3 of the radius of the concentric circle array. On the one hand, this design can make the reaction solution flow to the outer circle in the process of hybridization to improve the hybridization efficiency; on the other hand, it can make the eluent flow to the oute...

Embodiment 3

[0036] It is basically the same as Example 2, except that the height difference between the innermost ring and the outermost ring forming the concentric circle array on the carrying layer is 1 / 5 of the radius of the concentric circle array, and the carrying layer is a glass slide layer .

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Abstract

The invention discloses a gene chip with high-flux detection. The gene chip comprises a solid-phase carrier layer and a gene chip area arranged on the solid-phase carrier layer, wherein a probe arrayis arranged on the gene chip area and is of a concentric circle array, and probe dots on adjacent two circular rings forming the concentric circle array are of staggered arrangement. According to thegene chip with high-flux detection, disclosed by the invention, the probe array is of the concentric circle array, and probe array dots of adjacent two circular rings are of staggered arrangement, sothat influence on final signal identification caused by mutual interference between adjacent two dots during probe synthesis can be avoided, and then, the accuracy of detection is improved; and the structure is simple, the use is convenient, the difficulty of experimentation is lowered, and the accuracy of detection results is improved.

Description

technical field [0001] The invention relates to a gene chip for high-throughput detection, which belongs to the field of gene detection. Background technique [0002] With the implementation of the Human Genome (Sequencing) Project and the rapid development of molecular biology-related disciplines, more and more genome sequences of animals, plants, and microorganisms have been determined, and gene sequence data is growing rapidly at an unprecedented rate. Gene chip detection has been widely used in the fields of disease diagnosis and treatment, drug screening, forensic identification, and environmental detection due to its high-throughput, rapid and efficient detection. Gene chips use a large number of specific oligonucleotide fragments or gene fragments as probes, which are regularly fixed on solid supports such as silicon wafers, glass wafers, plastic wafers or nylon substrates combined with photoelectric measurement devices to form a two-dimensional The array hybridizes ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C40B40/06C12Q1/6837
Inventor 张明航李佼佼苏雪峰赵晓芳季少平郭向前王宇峰葛斌文
Owner 江苏苏博生物医学科技南京有限公司
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