aiv H5 subtype monoclonal antibody, hybridoma cells and chromatography test strips
A monoclonal antibody and hybridoma cell technology, applied in the direction of analytical materials, antiviral immunoglobulin, biochemical equipment and methods, etc., can solve the problems of expensive equipment, cumbersome operation, and high requirements for experimental conditions, and achieve good specificity and potency, and the effect of improving detection speed
Active Publication Date: 2020-06-30
SHENZHEN CUSTOMS ANIMAL & PLANT INSPECTION & QUARANTINE TECH CENT +1
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AI Technical Summary
Problems solved by technology
Traditional methods mainly include virus isolation, culture, identification and serological diagnosis, but their disadvantages are cumbersome operations, time-consuming and poor reproducibility of results
Molecular biology methods, such as RT-PCR and gene chip technology, have been applied to detect AIV, which have the characteristics of specificity, sensitivity, simplicity and quickness, etc., but require high experimental conditions, expensive instruments and equipment, and low operating skills of personnel. There are also requirements, and it is not easy to popularize and promote in grassroots veterinary departments
Method used
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Embodiment
[0028] 1. Main test materials
[0029] 1. Immunization antigen
[0030] H5N1 (Re-6) and H5N1 (Re-7) were purchased from Harbin Veterinary Research Institute; H5N2 (vaccine strain) was purchased from Guangdong Dahuanong Animal Health Products Co., Ltd.; H5N1 (FJ) was collected from Fujian and inspected by Shenzhen Entry-Exit Inspection Preserved and provided by the Animal and Plant Inspection and Quarantine Technology Center of the Bureau of Quarantine; H5N1 (HK) was collected from Hong Kong and preserved and provided by the Animal and Plant Inspection and Quarantine Technology Center of the Shenzhen Entry-Exit Inspection and Quarantine Bureau.
[0031] 2. Experimental Animals and Cells
[0032] Myeloma SP2 / 0 cells were preserved and provided by the Animal and Plant Inspection and Quarantine Technology Center of Shenzhen Entry-Exit Inspection and Quarantine Bureau; BALB / c mice were purchased from Guangdong Medical Experimental Animal Center.
[0033] 3. Reagents
[0034] Fre...
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The application of the invention discloses an AIV H5 sub-type monoclonal antibody, a hybridoma cell and a chromatography test paper strip. The AIV H5 sub-type monoclonal antibody disclosed by the invention is produced through secretion of a hybridoma cell H5HA 25 of which the preservation number is CCTCC NO.C2017262. The AIV H5 sub-type monoclonal antibody has favorable specificity and titer; through the prepared fluorescent microsphere marked quick immunity detection test paper, the detection speed and the detection sensitivity are increased, and on-the-spot detection of AIV H5 sub-type is realized. The AIV H5 sub-type monoclonal antibody disclosed by the application fills the vacancy of AIV H5 sub-type fluorescence immune chromatography detection, and a base is established for immunologyresearch of the AIV H5 sub-type.
Description
technical field [0001] This application relates to the field of monoclonal antibodies, in particular to a monoclonal antibody used for the detection of AIV H5 subtype, a hybridoma cell secreting the monoclonal antibody, and a chromatographic test strip based on the AIV H5 subtype monoclonal antibody . Background technique [0002] Avian influenza (Avian Influenz, abbreviated as AI) is an infection and / or disease syndrome of birds caused by type A avian influenza virus (abbreviated as AIV). There are three kinds of highly pathogenic (Highlypathogenic Avian Influenza, HPAI), low pathogenic (LPAI) and non-pathogenic. The H5NA subtype virus strain belongs to HPAI, and its harm to the poultry industry makes the rapid diagnosis of this subtype AIV a research hotspot. [0003] Hemagglutinin (HA) is the surface glycoprotein (75Ku) of AIV. It is one of the main components of the AIV capsule fibrils. It is directly involved in the pathogenic process of AIV. Point mutations in the ge...
Claims
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IPC IPC(8): C07K16/10C12N5/20G01N33/569G01N33/577
Inventor 王津津卢奕良孙洁蔡良语陈兵郑晓聪秦智锋
Owner SHENZHEN CUSTOMS ANIMAL & PLANT INSPECTION & QUARANTINE TECH CENT