A kind of method for extracting total flavonoids in scutellaria scutellariae with subcritical water
A technology of subcritical water and scutellaria, applied in material separation, analysis materials, instruments, etc., can solve the problems of complex recovery, environmental toxicity, and low extraction rate of polar components, and achieve simple operation process, easy implementation, and good extraction effect of effect
Active Publication Date: 2022-08-05
SHANDONG ANALYSIS & TEST CENT
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Problems solved by technology
[0002] Erigeron breviscapus is also known as Erigeron breviscapus and East chrysanthemum. The active ingredients of flavonoids in breviscapus have the functions of increasing blood flow, improving microcirculation, dilating blood vessels, reducing blood viscosity, reducing blood fat, promoting fibrinolysis, antithrombotic, antiplatelet aggregation, etc. , the methods for extracting the flavonoids of Breviscapus breviscapus in the prior art include water reflux extraction, supercritical CO 2 Extraction method, ultrasonic extraction method, two-phase and ultrasonic coupling extraction method, macroporous adsorption resin method, these methods are mainly organic solvent extraction and extraction with water as solvent at normal temperature and pressure, which will cause great harm to the environment , recycling is complicated, and the price is also high
These methods have the problem of low extraction rate of polar components with molecular weight greater than 500
[0003] Comparative document 1, 201511007502.8, records that Erigeron breviscapine uses ethanol or water reflux to extract, and then separates the extract with a styrene macroporous resin column to obtain a clear paste containing scutellarin; comparative document 2 In 200310113536.6, it is recorded that the coarse powder of Erigeron breviscapus is taken, extracted with alkaline solution at normal temperature, extracted by reflux, and adsorbed by polyamide column to obtain the extract. The extraction method of the above-mentioned literature has a low extraction rate of total flavonoids. There is no method with a higher extraction rate of total flavonoids from Breviscapus
Method used
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Embodiment 1
[0065] 1) Separate the purchased Dengzhan flowers according to their parts, and then put them into a pulverizer for pulverization, and then pour them into sieves of different specifications to sieve to obtain samples of different parts with a particle size of 70-100 mesh, and then put them into sample bags according to the number. Store in a dry and dark place for later use;
[0066] 2) Accurately weigh 0.2g (±0.0005g) of the scutellariae sample, add it to a stainless steel reaction kettle with a teflon lining, then add 4.5mL of deionized water, tighten the reaction kettle and put it into a 130°C In the heating and stirring controller, after 5 minutes, take out the reactor and cool it to room temperature with flowing water, open the reactor and rinse the top solution of the reactor three times with 1.0mL of pure methanol, extract the rinse solution into a 10mL colorimetric tube, and then use Extract the sample solution with pure methanol and make up to the mark to obtain the e...
Embodiment 2
[0070] The difference from Example 1 is that the particle size in step 1) is 20-40 mesh.
Embodiment 3
[0072] The difference from Example 1 is that the particle size in step 1) is 40-70 mesh.
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Abstract
The invention relates to a method for extracting total flavonoids in scutellaria scutellariae by subcritical water. The scutellaria scutellariae is separated according to the parts, and pulverized respectively to obtain samples pulverized by different parts; the samples are put into a subcritical water extraction device, and added Ionized water, the volume ratio of scutellaria brevis sample and deionized water is 1:15-1:30, after extraction for 5-30min, use pure methanol to rinse the reaction kettle, use pure methanol to extract the rinse, and take the supernatant of the extract, The sample solution was obtained by organic membrane filtration, and the sample solution was detected by HPLC, and the obtained samples included chlorogenic acid, scutellarin, isosalvianolic acid B, isosalvianolic acid A, scutellarin, isosalvianolic acid C, wild scutellarin The total flavonoids of baicalein, the extraction rate of total flavonoids is 10.76%, and HPLC can separate and purify each component.
Description
technical field [0001] The invention belongs to the technical field of plant component extraction, and in particular relates to a method for extracting total flavonoid components in scutellaria brevis through subcritical water. Background technique [0002] Dengzhanhua is also known as Dengzhan Asarum and Dongju. The active ingredients of flavonoids in Dengzhanhua can increase blood flow, improve microcirculation, dilate blood vessels, reduce blood viscosity, lower blood lipids, promote fibrinolysis, resist thrombosis, and resist platelet aggregation. , in the prior art, the methods for extracting the flavonoids of breviscapae include water reflux extraction, supercritical CO 2 Extraction method, ultrasonic extraction method, aqueous two-phase and ultrasonic coupling extraction method, macroporous adsorption resin method, these methods are mainly organic solvent extraction and extraction with water as solvent at normal temperature and pressure, which are very toxic to the en...
Claims
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IPC IPC(8): G01N30/02G01N30/06
Inventor 程燕薛富民于帅王学重陈辉郝翠郭丹丹
Owner SHANDONG ANALYSIS & TEST CENT