Cell culture composite film and preparation method and application thereof

A cell culture and composite membrane technology, applied in tissue cell/virus culture devices, biochemical equipment and methods, and other household appliances, etc., can solve problems such as economic loss, expensive biological products, membrane damage and leakage, etc.

Inactive Publication Date: 2019-06-14
杭州九龄科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In the field of bioreactors, it is mainly used for the cultivation of mammalian cells. At present, perfusion culture and fed-batch culture are mainly adopted, and various culture methods need to be stirred; in addition, the membrane is also made into a disposable storage solution Bags are used for storage and transshipment. In the field of liquid transportation, there are often relatively large operating pressures, which are convenient for fluid transmission and transportation. If the membrane strength is not enough, it cannot withstand these mechanical stresses and cause membrane damage and leakage. Due to biological products It is absolutely unacceptable that it is extremely expensive and causes great economic loss, so the prepared membranes need to be able to withstand various mechanical stresses so that they can be used in a wide range of applications

Method used

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  • Cell culture composite film and preparation method and application thereof
  • Cell culture composite film and preparation method and application thereof
  • Cell culture composite film and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The present invention adopts multi-layer co-extrusion to prepare the multi-layer film whose extruded thickness is 300um, and its structural composition of this multi-layer film:

[0027] The outermost layer of PEEK with a thickness of 50um is 450G produced by VICTREX Company, its density is 1.26g / cm3, water absorption: 0.07%, test conditions: 23°C, 24hr, 3.20mm, test method: ISO62); Bending strength: 165MPa, test condition: 23℃, test method: ISO178); tensile stress: 100MPa, test condition: yield, 23℃, test method: ISO527-2); tensile strain: 45MPa, test condition: fracture, 23°C, test method: ISO527-2).

[0028] Oxygen barrier layer EVOH with a thickness of 50um, which is F171 produced by Kuraray Corporation of Japan, has a density of 1.19g / cm3, an ethylene content of 32%, and elongation at break: 14.4% (test method: ISO-527), Water vapor transmission rate: 40-80g*30u / m 2 *24hrs, test conditions: 40℃, 90%RH; oxygen transmission rate: 0.4cm3*20u / m 2 *24hrs*atm, test co...

Embodiment 2

[0034] The present invention adopts the multi-layer film prepared by multi-layer co-extrusion, and its structural composition of this multi-layer film:

[0035] The outermost layer of PEEK with a thickness of 10um, which is 450G produced by VICTREX company, has a density of 1.26g / cm3, water absorption: 0.07%, test conditions: 23°C, 24hr, 3.20mm, test method: ISO62); Bending strength: 165MPa, test condition: 23℃, test method: ISO178); tensile stress: 100MPa, test condition: yield, 23℃, test method: ISO527-2); tensile strain: 45MPa, test condition: fracture, 23°C, test method: ISO527-2).

[0036] Oxygen barrier EVOH with a thickness of 15um, which is produced by Kuraray Corporation of Japan is F171, with a density of 1.19g / cm3, an ethylene content of 32%, and elongation at break: 14.4% (test method: ISO-527), Water vapor transmission rate: 40-80g*30u / m 2 *24hrs, test conditions: 40℃, 90%RH; oxygen transmission rate: 0.4cm3*20u / m 2 *24hrs*atm, test conditions: 20°C, 65%RH.

...

Embodiment 3

[0041] Embodiment 3: mechanical stress experiment

[0042] Equipment: Swing test bag tightness

[0043] The 2D bag prepared with the multilayer film (code A) of Example 1 and having a volume of 25 liters is filled with 25 liters of water and placed on a motion platform that swings left and right, with a swing angle of plus or minus 12° and a speed of 45 r / min, run until leak, test for fluid coming out of the bag.

[0044] The test is the same as film No. A under the same conditions, except that the same 2D bag is prepared from the commercially available pureflex multilayer film (No. B) of the American millipore company, and the test conditions are exactly the same.

[0045] The test is the same as film No. A under the same conditions, except that the same 2D bag is prepared from the commercially available flexsafe multilayer film (No. C) of German company Sartorious, and the test conditions are exactly the same.

[0046] The test is the same as the film No. A under the same...

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Abstract

The invention relates to a cell culture composite film and a preparation method and application thereof. The cell culture composite film comprises a core layer, a first binding layer, an oxygen-resisting layer, a second binding layer and an anti-abrasion outer layer which are sequentially bonded from inside to outside, and is prepared through technologies such as multilayer co-extrusion or embedding or lamination; and the film prepared through the method can be used for manufacturing various single-use bioreactors, storage containers and transferring containers for cell culture, and has very little dissolved matter and educt, great biocompatibility and very low cytotoxicity, a selected core layer material is biodegradable, the production method is simple, the cost is low, and the cell culture composite film is an environment-friendly polymer thin film material and is particularly suitable for the biopharmaceutical industry.

Description

technical field [0001] The invention relates to the field of biopharmaceutical cell culture containers, in particular to a cell culture composite membrane and its preparation method and application. Background technique [0002] In the biopharmaceutical industry, disposable bags are widely used in the field of biopharmaceutical cell culture, such as disposable bioreactors or containers for storing or transporting cell fluids, such as cell media, buffers, cell culture media, etc.; The rapid development of technology has greatly promoted the rapid growth of its usage. These disposable products are flexible, convenient, fast, and low in cost, without verification. They are ideal substitutes for traditional stainless steel containers and various material boxes. The handling and delivery of raw materials, buffers, intermediates and the final finished drug greatly reduces the one-time huge hardware investment cost of pharmaceutical companies and greatly reduces the initial risk. ...

Claims

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Application Information

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IPC IPC(8): B32B27/36B32B27/08B32B7/12B32B27/30B32B27/28B32B33/00B29D7/01C12M3/00
Inventor 唐勇红刘锋江润生郭爱连
Owner 杭州九龄科技有限公司
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