Method for cultivating yangtze river delta white goathair follicle stem cells in tissue explant method

Abstract

The invention provides a method for cultivating yangtze river delta white goathair follicle stem cells in a tissue explant method. When the method is adopted to separate cells, a cell line is good ingrowth and division state, and presents a typical paving stone shape. Primary cells climb out of tissue explants faster, and a user can observe in the third day that some cells climb out of the tissueexplants for growth and can observe in the fourth day that many cells climb out around each tissue explant in the field of a microscope. On the fifth day, the user can observe in the field of the microscope that a large cluster of cells surrounds the tissue explants, and the first subculture is performed at the moment. The constructed yangtze river delta white goathair follicle stem cell line hasstrong activity and high positive rate of hair follicle stem cell markers, which can provide a good practical model for study of the skin cell gene expression and pathway regulation, the growth and differentiation mechanism of the skin accessory structure, especially the interaction mechanism between the main cells of the skin.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for cultivating hair follicle stem cells of white goats (Haimen goats) in the Yangtze River Delta by a simple and rapid method of separating tissue blocks. Background technique [0002] Mammalian hair is differentiated from adult stem cells in the hair follicle, which undergoes two processes of shedding and regeneration. The hair follicle is an ideal model for studying adult stem cells. Hair follicle stem cells widely exist in the structure of skin hair follicles. They are primitive cells in hair follicles. They have multi-directional differentiation potential and can produce cells needed by the body through their own division and proliferation. Hair follicle stem cells are located in the hair follicle bulge, and are closely related to hair cycle maintenance, tissue maintenance and renewal, and skin damage repair. Hair follicle (HF) is an ideal system for studyi...

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Problems solved by technology

The main separation methods are mechanical separation and enzymatic digestion. Although both methods can obtain hair follicle stem cells, there are still some shortcomings such as poor attachment of tissue blocks and damage to cells due to enzymatic digestion and mechanical separation. There are methods to describe the isolation process of goat hair follicle stem cells, most of which focus on fine-wool sheep and cashmere goats, and the hair follicle structures of sheep and goats are different in size and shape under the microscope, which makes the isolation method of hair follicle stem cells not fully applicable between species

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