Enzymatic method integrated extraction method of functional polysaccharide and procyanidin in longan peel

An extraction method and proanthocyanidin technology are applied in the field of enzymatic integrated extraction of functional polysaccharides and proanthocyanidins in longan pericarp, which can solve the problems of lack of integrated technology for continuous preparation of proanthocyanidins, achieve high-value utilization, improve proanthocyanidin yield and polysaccharide yield. rate, the effect of comprehensive development promotion

Inactive Publication Date: 2019-06-14
FUZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

When studying the chemical composition of a substance, organic solvents are usually used to extract proanthocyanidins, and hot water is used to extract polysaccharide components, but there is a lack of related inventions for the integrated technology of continuous preparation of proanthocyanidins and polysaccharides

Method used

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  • Enzymatic method integrated extraction method of functional polysaccharide and procyanidin in longan peel
  • Enzymatic method integrated extraction method of functional polysaccharide and procyanidin in longan peel
  • Enzymatic method integrated extraction method of functional polysaccharide and procyanidin in longan peel

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Embodiment 1

[0028] A method for enzymatically integrated extraction of functional polysaccharides and proanthocyanidins in longan pericarp, comprising the following steps: pulverizing the longan pericarp to produce peel residue, passing through a 100-mesh sieve, and then extracting by hot water enzymatic method, using 0.6wt% composite enzyme A (cellulase / Hemicellulase / pectinase 2:1:2), liquid to material ratio 20:1, extraction time at 55°C for 2.5 hours, centrifugation (5000rpm, 20 minutes), take the supernatant, get supernatant A, supernatant Concentrate solution A to 1 / 6 of the original volume to obtain concentrated solution A, add 0.3 times (m / v) sodium chloride and 1 time (v / v) 95vol% ethanol to concentrated solution A, stir and mix well and let stand The layers are separated by a separating funnel, the supernatant is procyanidin, and the lower layer is the crude polysaccharide solution. Concentrate the supernatant to 1 / 10 of the original volume to obtain concentrated solution B. The...

Embodiment 2

[0031] A method for enzymatically integrated extraction of functional polysaccharides and proanthocyanidins in longan pericarp, comprising the following steps: pulverizing the longan pericarp to produce peel residue, passing through a 100-mesh sieve, and then extracting by hot water enzymatic method, and using 0.55wt% composite enzyme A (cellulase / Hemicellulase / pectinase 2:1:2), liquid to material ratio 20:1, extraction time at 55°C for 3 hours, centrifugation (5000rpm, 20 minutes), take the supernatant, get supernatant A, supernatant Concentrate solution A to 1 / 8 of the original volume to obtain concentrated solution A, add 0.3 times (m / v) sodium chloride and 1 time (v / v) 95vol% ethanol to concentrated solution A, stir and mix well and let stand The layers are separated by a separating funnel, the supernatant is procyanidin, and the lower layer is the crude polysaccharide solution. Concentrate the supernatant to 1 / 10 of the original volume to obtain concentrated solution B. ...

Embodiment 3

[0034]An enzymatic integrated extraction method for functional polysaccharides and proanthocyanidins in longan peel, comprising the following steps: pulverizing the longan peel into peel residue, passing through a 100-mesh sieve, and then extracting it by hot water enzymatic method, and using 0.65% compound enzyme A (cellulase / half enzyme) Cellulase / pectinase 2:1:2), the ratio of liquid to material is 20:1, the extraction time is 2.9 hours at 55°C, centrifuged (5000rpm, 20 minutes), and the supernatant is taken to obtain the supernatant A, the supernatant Concentrate A to 1 / 10 of the original volume to obtain concentrated solution A. Add 0.3 times (m / v) sodium chloride and 1 time (v / v) 95vol% ethanol to concentrated solution A, stir and mix well, and then stand to separate The layers are separated by a separating funnel, the supernatant is procyanidin, and the lower layer is the crude polysaccharide solution. Concentrate the supernatant to 1 / 10 of the original volume to obtain...

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Abstract

The invention provides an enzymatic method integrated extraction method of functional polysaccharide and proanthocyanidin in longan peel, and belongs to the technical field of biological extraction. According to the enzymatic method integrated extraction method, the functional polysaccharide and the proanthocyanidin are jointly extracted by hot-water enzymatic extraction, concentration, standing and layering. The method is simple and rapid in extraction process and low in cost, achieves high-value resource utilization of longan peel waste, increases the deep processing benefit of longan, and has the positive promotion effect on comprehensive development of longan byproducts.

Description

technical field [0001] The invention belongs to the technical field of biological extraction, in particular to an enzymatic integrated extraction method of functional polysaccharides and proanthocyanidins in longan pericarp. Background technique [0002] Longan is a kind of nutritious fruit that can be eaten fresh and processed. With the continuous improvement of agricultural production technology, the annual output of longan is rising steadily. Nowadays, longan has been widely used in brewing or producing canned longan, dried longan and other processed products. Due to the lack of effective means of utilization, the peel and core, which account for more than 17% of the fresh weight of the longan fruit, are discarded as waste. There are more than 20,000 tons / year of discarded longan peels and pits in the country, which are in urgent need of comprehensive utilization. Longan peel and pit are rich in biologically active substances, especially proanthocyanidins and functional...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C07D311/62
Inventor 付才力陈元胜陈舒婷王腾晏亦岚
Owner FUZHOU UNIV
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