Method for extracting ganoderma triterpene and ganoderma lucidum polysaccharide from ganoderma lucidum

Abstract

A method for extracting ganoderma triterpene and ganoderma lucidum polysaccharide from ganoderma lucidum comprises the steps as follows: (1) pretreatment of raw materials: placing crushed ganoderma lucidum fruiting bodies in a sodium hydroxide-aqueous solution for extraction, and performing filtration after ultrasonic treatment to obtain a filtrated material and an extract liquid; (2) pH adjustment: adjusting pH of the extract liquid to 2.0-4.5; (3) dynamic reflux adsorption: performing dynamic reflux adsorption on the extract liquid obtained in step B, and after adsorption, collecting an effluent I; (4) staged elution: dividing the elution process into four stages, mixing and collecting eluates III at the first three stages, and collecting an eluate III which is a ganoderma triterpene extract liquid at the fourth stage; (5) mixing the effluent I and the eluates II, adding ethanol for ethanol precipitation, and performing filtration, drying and crushing to obtain crude ganoderma lucidum polysaccharide. The extract rate of ganoderma triterpene acid and ganoderma lucidum polysaccharide and purity of ganoderma triterpene are effectively increased, the extraction scheme is easy to implement, and extracted products are safe and effective.

Description

technical field [0001] The invention belongs to the technical field of plant effective component extraction methods, and in particular relates to a method for extracting ganoderma triterpenoids and ganoderma polysaccharides from ganoderma lucidum. Background technique [0002] Ganoderma lucidum is the general name of fungi belonging to the genus Ganoderma lucidum of the family Non-Pleatophthora order Ganoderma lucidum; the customary name of Ganoderma lucidum refers to the fruiting body part of Ganoderma lucidum in the genus Ganoderma lucidum. Ganoderma lucidum was first recorded in "Shen Nong's Herbal Classic", and it was listed as the top grade in medical books of all dynasties Ganoderma lucidum is a kind of Chinese medicinal material included in the "Chinese Pharmacopoeia", and it is a fungus that is used for both food and medicine. The polysaccharides, triterpenes, nuclear Glycosides, alkaloids, amino acid polypeptides, trace elements and other components are the material...

AI Technical Summary

Problems solved by technology

[0006] In the 1980s, researchers at home and abroad began to study the chemical components of Ganoderma lucidum in depth, and achieved certain results. So far, more than 200 compounds have been successfully isolated and their molecular structures identified; but these works are all carried out in laboratories. Yes, the amount of compound obtained is very small, which greatly affects the development and utilization of Ganoderma lucidum triterpenes. Therefore, it is of great theoretical and practical significance to study the industrialization technology of the extraction and purification of triterpenoids

[0008] The traditional alcohol extraction method usually needs to consume a large amount of extraction solvents, and the use of a large amount of organic solvents will have adverse effects on the human body and the environment

[0009] Patent No. CN1560072A discloses a method for preparing Ganoderma triterpenes and Ganoderma polysaccharides from Ganoderma lucidum. After the dried Ganoderma lucidum is crushed, supercritical CO 2 Extraction technology extracts Ganoderma lucidum triterpenoids, and then uses low-temperature extraction and rapid concentration technology to obtain Ganoderma lucidum polysaccharides, but adopts supercritical CO 2 The equipment required for the extraction technology is relatively large, the operation is difficult, and the production cost is high

[0010] Compared with other fungi, Ganoderma lucidum fruiting body has a high degree of lignification and a complex and compact structure, while triterpenes and Ganoderma lucidum polysaccharides are distributed inside the cells. The extraction rate is low through traditional extraction methods, and most of the active ingredients are retained in the residue. , resulting in a waste of resources

[0011] Patent No. CN105294875B discloses a method for extracting Ganoderma lucidum polysaccharides from Ganoderma lucidum fruiting bodies. After the Ganoderma lucidum fruiting bodies are pulverized, the cellulose and lignin that make up the cell wall are reacted with the oxidation system of sodium chlorite-glacial acetic acid. Digestion and fracture release more active substances, which greatly improves the extraction efficiency of polysaccharides. However, Ganoderma lucidum triterpenoids and polysaccharides are mostly used in medicine and health care, and sodium chlorite is highly carcinogenic. Extracting products with this method is likely to cause safety hazards

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