Composite gold nanoclusters phototherapy agent capable of being simultaneously used for near infrared biological windows I and II and preparation method thereof

A composite gold and nanocrystal technology, which is applied in the direction of drug combinations, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc., can solve the problems of complex preparation process and limited material application, and achieve excellent light and heat stability performance and excellent light-to-heat conversion efficiency

Active Publication Date: 2019-06-18
UNIV OF SCI & TECH OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But in general, there are still very few noble metal phototherapeutic agents that can be used for NIR-II, and the preparation process of these phototherapeutic agents that have been reported is relatively complicated, which seriously limits the application of such materials in the biological field.

Method used

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  • Composite gold nanoclusters phototherapy agent capable of being simultaneously used for near infrared biological windows I and II and preparation method thereof
  • Composite gold nanoclusters phototherapy agent capable of being simultaneously used for near infrared biological windows I and II and preparation method thereof
  • Composite gold nanoclusters phototherapy agent capable of being simultaneously used for near infrared biological windows I and II and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 ([NPG] / [Au(III)] (molar ratio)=42):

[0034] 1mL HAuCl 4 (10mM) solution was added to 8mL NPG (8mg / mL) sodium hydroxide solution (0.75mM), stirred vigorously for 2min and mixed evenly. 16C), centrifuge at 12000rpm for 10min, collect the bottom product, wash with N,N-dimethylformamide and deionized water three times, and then disperse it in 2mL deionized water for later use. The TEM photo of the product AuNCC is as follows figure 1 As shown, the average particle size is about 26 nm. Its high-resolution TEM pictures are as follows figure 2 As shown, the lattice spacings of 0.232nm and 0.202nm correspond to (111) and (200) of Au nanocrystals, respectively (H.Wang et al., Advanced Materials 2016, 28, 8218-8226). The XPS spectrum of the product is as image 3 As shown, compared with the pure PNPG XPS spectrum, the appearance of Au4d and Au4f peaks ( image 3 a) and Au 4f ( image 3 b) The difference in binding energy between the two peaks is 3.7eV (Au4f 7 / ...

Embodiment 2

[0035] Embodiment 2 ([NPG] / [Au(III)] (molar ratio)=21):

[0036] 1mL HAuCl 4 (20mM) solution was added to 8mL NPG (8mg / mL) sodium hydroxide solution (0.75mM), stirred vigorously for 2min, and mixed evenly. -16C), centrifuge at 12000rpm for 10min, collect the bottom product, wash with N,N-dimethylformamide and deionized water three times, and then disperse it in 2mL deionized water for later use. The TEM photograph of the product is as Figure 7 As shown, it is also a gold nanocrystal cluster wrapped by PNPG, and the UV-VIS-NIR spectrum of its aqueous dispersion is as follows Figure 8 As shown, AuNCC shows strong absorption in both NIR-I and NIR-II windows.

Embodiment 3

[0037] Embodiment 3 ([NPG] / [Au(III)] (molar ratio)=84):

[0038] 1mL HAuCl 4 (5mM) solution was added to 8mL NPG (8mg / mL) sodium hydroxide solution (0.75mM), vigorously stirred for 2min, and mixed evenly. -16C), centrifuge at 12000rpm for 20min, collect the bottom product, wash with N,N-dimethylformamide and deionized water three times, and then disperse it in 2mL deionized water for later use. The TEM photograph of the product is as Figure 9 As shown, it is also a gold nanocrystal cluster wrapped by PNPG, and the UV-VIS-NIR spectrum of its aqueous dispersion is as follows Figure 10 As shown, it is shown that AuNCC has a strong absorption in the NIR-I window and a relatively weak absorption in the NIR-II window.

[0039] The photothermal conversion performance characterization of the AuNCC prepared in Example 4 Example 1

[0040] With a power density of 1W cm -2 and 0.3W cm -2 The 808nm (NIR-I) or 1064nm (NIR-II) laser irradiates 1mL of AuNCC aqueous dispersion (100μg / ...

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Abstract

The invention provides a preparation method of a composite gold nanocluster phototherapy agent, the method comprises the following steps of: mixing aqueous solution of HAuC l4 and alkaline solution ofN-phenylglycine to obtain a gold nanocluster coated with poly N-phenylglycine. The invention provides a convenient and simple method for obtaining a composite gold nanoclusters which has effective absorption in both NIR-I and NIR-II windows at room temperature by a pot method, and the composite gold nanoclusters has the excellent photothermal conversion efficiency and photothermal stability in two near infrared rays, meanwhile, surrounding O 2 molecules can be excited by near infrared laser irradiation to generate singlet oxygen, so that the composite gold nanoclusters in the near infrared Iand II windows both have dual therapeutic effects of photothermal and photodynamic. The preparation method and the phototherapy agent are expected to provide an efficient phototherapeutic agent for cancer treatment in the future.

Description

technical field [0001] The invention belongs to the technical field of polymer biomaterials, and in particular relates to a composite gold nanocrystal cluster phototherapeutic agent which can be used in both near-infrared biological windows I and II and a preparation method thereof. Background technique [0002] In recent years, the latest development targets in the field of phototherapy are near-infrared (NIR) light-mediated photothermal therapy (PTT) and photodynamic therapy (PDT). NIR light with a wavelength of 650-1350nm can effectively penetrate biological tissues such as skin and blood (S.Nie et al., Nature Nanotechnology 2009, 4, 710-711) for non-invasive and precise remote cancer treatment (F.Y.Li et al. ., Nature Communications 2016, 7, 10437). So far, there have been a lot of research to find and develop various photothermal agents (PTA) or photosensitizers (PS) that absorb near-infrared light and use them to treat tumors buried in deep tissues (X.W.Ge et al., Nan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K9/51A61K47/34A61P35/00
Inventor 汪谟贞雷珊葛学武
Owner UNIV OF SCI & TECH OF CHINA
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