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Culture method of sulfate-reducing bacterial strain, sulfate-reducing bacterial strain and application

A culture method and sulfate technology, applied in the field of microorganisms, can solve the problems of limited expansion application, low governance efficiency, and poor general governance effect.

Active Publication Date: 2022-03-18
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Therefore, the environmental behavior of organic arsenic pollutants and the safe restoration technology for such pollutants need to be researched and developed urgently. Nowadays, there is no good biological treatment method for organic arsenic pollutants, and the general treatment effect is not good. low, which limits its further extended application

Method used

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  • Culture method of sulfate-reducing bacterial strain, sulfate-reducing bacterial strain and application
  • Culture method of sulfate-reducing bacterial strain, sulfate-reducing bacterial strain and application
  • Culture method of sulfate-reducing bacterial strain, sulfate-reducing bacterial strain and application

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Effect test

Embodiment 1

[0045] The method for separating and purifying sulfate-reducing bacterial strains specifically comprises the following steps:

[0046] The strain SRB-2 obtained in this example was enriched and cultured from DPAA-contaminated flooded soil, and isolated and screened.

[0047] Enrichment culture method: Take 5mL of the mixed soil suspension and transfer it to a serum bottle containing 95mL of modified Postgate B medium, exchange the upper layer of air with nitrogen, and seal it with an aluminum cover with a Teflon inner pad Put it in a constant temperature incubator and let it stand for 1 to 2 weeks, until the medium turns black. The production of H2S was detected by wetted lead acetate test paper to prove that the cultivation was good. Use the enrichment medium to enrich the bacterial solution for 2 to 3 times.

[0048] The medium for enrichment culture is modified Postgate B medium, its components are: its components are: K 2 HPO 4 ·3H 2 O0.5g, NH 4 Cl 1.0g, Na 2 SO 4 ...

Embodiment 2

[0062] The cultivation method and specific effect evaluation of the sulfate-reducing bacterial strain are carried out according to the following steps:

[0063] 1) Prepare Postgate B medium: K 2 HPO 4· 3H 2 O 0.3g / L, NH 4 Cl 1.5g / L, Na 2 SO 4 2g / L, CaCl 2 2H 2 O 0.06g / L, MgCl 2 ·6H 2 O 0.2g / L, sodium lactate 4g / L, yeast extract 0.5g / L, solvent is water, adjust pH 7.0.

[0064] 2) Add diphenylarsenic acid (DPAA) to the above-mentioned Postgate B medium, the concentration after addition is 2mg / L, and add goethite at the same time (addition of iron mineral is to simulate the DPAA in the soil under real conditions is easy to adsorb on iron minerals in the soil), and inoculate the Clostridium sp.SRB-2 bacterial solution cultivated to the logarithmic phase according to the inoculum amount of 5%, and set the control CK without inoculation (the blank medium without SRB-2 bacteria ), replace the upper layer of air with high-purity nitrogen, seal it with a polytetrafluoroethy...

Embodiment 3

[0069] The culture method of sulfate-reducing bacterial strain is carried out according to the following steps:

[0070] 1) Prepare Postgate B medium: K 2 HPO 4 ·3H 2 O 1g / L, NH 4 Cl 0.5g / L, Na 2 SO 4 4g / L, CaCl 2 2H 2 O 0.02g / L, MgCl 2 ·6H 2 O 0.6g / L, sodium lactate 2g / L, yeast extract 1.5g / L, solvent is water, adjust pH 7.0.

[0071] 2) Add diphenylarsenic acid (DPAA) to the above-mentioned Postgate B medium, the concentration after addition is 10mg / L, and add goethite at the same time (adding iron minerals is to simulate the DPAA in the soil under real conditions is easy to adsorb on on the iron minerals in the soil), and inoculate the Clostridiumsp. -2 bacterial blank culture medium), replace the upper layer of air with high-purity nitrogen, seal it with a polytetrafluoroethylene inner pad and an aluminum cover, place it in a shaking incubator, and culture it in the dark at 35°C and 180r / min.

[0072] Subsequent analysis of SO in the culture system 4 2- After...

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Abstract

The invention relates to the field of microorganisms, in particular to a method for cultivating sulfate-reducing bacterial strains, the sulfate-reducing bacterial strain and applications. The strain is preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee, the preservation number is: CGMCC No: 14043; the preservation time is: April 17, 2017. The strain has excellent degradation characteristics and is widely used in the degradation and conversion of organic arsenic pollutants, especially diphenyl arsenic acid. The degradation conversion rate of DPAA in the system can reach 41.8%. The bioremediation of groundwater provides new resources and ideas, and has a good application prospect.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a method for cultivating sulfate-reducing bacterial strains, sulfate-reducing bacterial strains and applications. Background technique [0002] Sulfate-Reducing Bacteria (SRB) is a group of anaerobic microorganisms that use organic matter such as lactic acid or pyruvic acid as electron donors and sulfate as terminal electron acceptors to reproduce under anoxic or anaerobic conditions. , Widely present in anoxic environments such as fields, paddy fields, lakes, river sediments, and seabed mud. [0003] In recent years, chemical weapons buried after wars often lead to serious environmental pollution incidents, among which arsenic pollution is the most common. Diphenylarsenic acid (DPAA) is a new type of environmental pollutant produced by arsenic chemical weapons after a series of hydrolysis and oxidation processes. In addition, arsenic-containing herbicides and pesticides widely us...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B09C1/10C02F3/34A62D3/02C12R1/145C02F101/30C02F103/06A62D101/24A62D101/28
Inventor 涂晨刘颖韦婧骆永明
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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