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Toluene degrading strain and application thereof

A technology of toluene and strains, applied in the field of bioengineering, can solve the problems of unclear ability to degrade toluene, few reports on microbial remediation of toluene-contaminated wastewater, and few studies on high-efficiency toluene-degrading strains, etc. , the effect of improving processing efficiency

Pending Publication Date: 2022-04-08
CHAMBROAD CHEM IND RES INST CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Microbial degradation is an important way and final means to solve toluene pollution. Screening highly efficient toluene-degrading strains is the key to the biodegradation method. Through literature and patent searches, there are not many studies on toluene-degrading strains with high efficiency, and most of them focus on toluene-containing low-concentration toluene. Research on the degradation of organic waste gas, there are not many reports on the microbial remediation of toluene-contaminated wastewater
[0005] The prior art CN103013885A discloses an immobilized benzene series compound bacterial agent compounded by four bacterial strains. Although it is mentioned that it has a certain degradation performance on benzene series, its ability to degrade toluene is not very clear, and the overall benzene series degradation ability still needs to be improved

Method used

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  • Toluene degrading strain and application thereof
  • Toluene degrading strain and application thereof
  • Toluene degrading strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The acquisition of embodiment 1 bacterial strain

[0030] Five strains with degradative effect on toluene were isolated from the samples taken around the coking unit of Shandong Dongbo Petrochemical Co., Ltd. and Shandong Qingyuan Environmental Protection Engineering Co., Ltd., and were separated and purified separately.

[0031] Follow-up toluene degradation experiments were carried out on the 5 strains to be selected, and finally a strain with high toluene degradation efficiency, easy cultivation and stable passage characteristics was screened out, and it was named YJY21-01.

[0032] The morphological characteristics of the strain are Gram-negative small bacilli, which are rod-shaped, capsulated, and without spores; the strain forms nearly round, medium milky-white colonies on ordinary nutrient agar, with irregular edges and flat colonies. When growing in medium, yellow insoluble matter will appear at the bottom of the bottle.

[0033] The inventor carried out 16SrDN...

Embodiment 2

[0035] The fermentation of embodiment 2 bacterial strains

[0036] (1) Strain activation: Take out the strains stored in the refrigerator at 4°C, and activate them at room temperature for 3h-4h. Since the strains are stored on the nutrient agar medium on the slant of the test tube, no additional nutrition is needed during the activation process.

[0037] (2) Preparation of liquid seeds: In a sterilized aseptic table, take 10ml of sterilized distilled water and add it to a bacteria-preserving test tube, blow and beat repeatedly to make a bacterial suspension, and then insert the bacterial suspension into 100ml of sterile conventional LB liquid culture Medium, shaking culture for 16-20h to prepare seed solution;

[0038] (3) Fermentation: Add fermentation medium into the fermenter, after sterilization, inoculate the seed solution at a volume ratio of 2‰, control the temperature during the fermentation process at 35°C, tank pressure at 0.05MPa, initial speed at 200rpm, dissolved ...

Embodiment 3

[0041]Example 3 Bacterial Strain Degradation Toluene Conditions

[0042] The bacterium liquid that adopts embodiment 2 to ferment carries out following experiment:

[0043] ①pH screening experiment

[0044] Prepare 50ml of inorganic salt medium containing 500mg / L toluene, the inoculation rate of the bacterial solution is 5%, the pH of the medium is set to 4.0, 5.0, 6.0, 7.0, 8.0, respectively, 30°C, 140rpm shaking culture for 24h to measure the remaining toluene , while setting up a blank control.

[0045] The result is as figure 1 It was shown that the degradation effect of the strain on toluene was the best when the pH was 7.0, and the degradation rate reached 93.59% in 24 hours.

[0046] ②Temperature screening experiment

[0047] Prepare 50ml of inorganic salt medium containing 500mg / L toluene, adjust the pH of the medium to 7.0, and the inoculation rate of the bacterial solution is 5%, set the temperature at 25°C, 28°C, 31°C, 34°C, 37°C, and shake at 140rpm After cult...

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Abstract

The invention belongs to the technical field of bioengineering, and provides a toluene degrading strain and application thereof. The strain can be used in treatment of toluene-containing wastewater in industries such as petrifaction, agriculture and chemistry, the strain is identified as Pseudomonas stutzeri through 16SrDNA, the strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) in June 2021, the strain code is YJY21-01, and the preservation number is CGMCC No.22756. The invention further discloses a method for preparing the toluene-containing wastewater containing the toluene-containing wastewater containing the toluene-containing wastewater containing the toluene-containing wastewater containing the toluene-containing wastewater containing the toluene-containing wastewater containing the toluene-containing wastewater containing the toluene-containing wastewater containing the toluene-containing wastewater containing the toluene-containing wastewater containing the toluene-containing wastewater containing the toluene-containing wastewater containing the toluene-containing wastewater. The strain disclosed by the invention has a relatively good degradation characteristic on toluene, does not cause secondary pollution to the environment, improves the treatment efficiency of toluene wastewater, and can be applied to the field of remediation of toluene-polluted wastewater and soil or toluene-containing organic waste gas.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and provides a toluene-degrading bacterial strain and application thereof. Background technique [0002] Toluene is a kind of benzene series. It is a kind of hydrophobic organic pollutant. It mainly comes from the production process of petroleum, chemical industry, paint, pesticide, medicine, etc. People will be acutely poisoned if they inhale high concentration of toluene in a short period of time. , resulting in symptoms such as dizziness, nausea, shortness of breath, weakness of hands and feet, and severe cases may lead to convulsions or even shock. Long-term exposure to toluene can cause chronic poisoning, neurasthenia syndrome, hepatomegaly, and chapped skin. Toluene is highly toxic and teratogenic to the human body, and it also harms the environment and pollutes air and water sources. Since the 1970s, the United States, the European Union, the World Health Organization, Japan and C...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34B01D53/84B01D53/72B09C1/10C12R1/38C02F101/32
CPCY02A50/20
Inventor 马娜娜杨传伦车树刚张心青司更花王建平王秀芝陈振发
Owner CHAMBROAD CHEM IND RES INST CO LTD
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