A kind of freeze-drying preservation method and application of wet paper-based sensor
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A preservation method and sensor technology, applied in instruments, scientific instruments, measuring devices, etc., can solve problems such as unsatisfactory timeliness, lack of preservation methods, and inactivation of biological substances, achieving rapid screening, high market value, high precision effect
Active Publication Date: 2020-07-28
XI AN JIAOTONG UNIV
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[0004] Nowadays, paper-based biosensors have made great contributions to tumor detection, but their timeliness is not satisfactory, and there is still a lack of good storage methods, so that they can only be prepared and used immediately. Some biological substances even It will be inactivated after natural drying and will not be detected
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preparation example Construction
[0035] A method for preparing a paper-based sensor element for detecting cancer according to an embodiment of the present invention, comprising:
[0036] According to the number of parts by mass, carboxylated multi-walled carbon nanotubes are 2 parts, EDC should be greater than or equal to 77 parts, and NHSS should be greater than or equal to 22 parts;
[0037] Specifically, prepare the reagents and materials needed for the preparation process: 2mg of carboxylated multi-walled carbon nanotubes, at least 4mL (0.1M) MES solution, 77mg of EDC powder, 22mg of NHSS powder, at least 100mL of PBS solution, Tween20 solution (concentration 0.05%) 10uL, 100uL of BSA solution (concentration 1%), two pieces of filter paper, two pieces of glass slides, gel, 0.5mL of antibody solution (concentration 0.01mg / mL).
[0038] Its preparation process comprises the following steps:
[0039] 1) Weigh 2 mg of carboxylated carbon nanotubes, and then pour the powder into a large centrifuge tube; you c...
Embodiment 1
[0067] A method for preparing a biosensor element for detecting cancer in an embodiment of the present invention comprises the following steps: preparing 2 mg of carboxylated multi-walled carbon nanotubes (purchased from Shanghai Macklin Biochemical Technology Co., Ltd., with an inner diameter of 5-12 nm and an outer diameter of 5-12 nm). diameter 30-50nm, length <10nm), add 0.4mmol EDC and 0.1mmol NHSS, and disperse in 4mL of 0.1mol MES solution. The dispersion solution was sonicated in a water bath for about 40 minutes in an ultrasonic instrument (Xinzhi SB-3200OTD), and then mixed evenly with a mixer (SCI LOGEX ICC8). After mixing, centrifuge for 10 minutes (3000rp / m) with a centrifuge (TD6, ordinary instrument), and then remove the supernatant. After the end, PBS buffer solution was added to the precipitate, centrifuged for 10 min (3000rp / m), and the supernatant was removed. Repeat the above PBS washing process for at least 5 times to remove excess EDC and NHSS in the pel...
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Abstract
The invention discloses a freeze-drying preservation method for a wet process paper-based sensor and application of the method. The method includes the following steps that: a protein protective agentis added into a wet paper-based sensor to be preserved, the temperature of a cold trap is decreased to be below -30 degrees Celsius, and the wet process paper-based sensor is placed in the cold trap;the cooling rate of the cold trap is set to be a predetermined value, temperature in the cold trap is lowered to be -60 degrees Celsius; freezing treatment is performed on the wet process paper-basedsensor in an environment of -60 degrees Celsius; the wet process paper-based sensor which has been subjected to freezing treatment is moved into the temperature rising portion of the cold trap, and is subjected to vacuuming operation, and the wet process paper-based sensor is warmed after a preset vacuum degree requirement is satisfied; when the wet process paper-based sensor is warmed to be glass transition temperature, the warming of the wet process paper-based sensor is stopped; the wet process paper-based sensor is warmed to be room temperature in an environment satisfying the preset vacuum degree requirement, and continuous desorption and drying is performed for more than 8 hours; and the wet process paper-based sensor is packaged and preserved. According to the preservation method of the of the present invention, the vacuum freeze-drying technique is adopted to perform vacuum freeze-drying on the filter paper-based cancer detecting sensor, so that the activity of biological tissues can be preserved to the greatest extent, and the long-term preservation of the sensor can be realized.
Description
technical field [0001] The invention belongs to the technical field of paper-based sensor storage, and in particular relates to a method for freeze-drying storage of wet-process paper-based sensors and an application thereof. Background technique [0002] Paper-based detection is favored by in vitro detection and related application detection due to its high detection rate, high selectivity, high sensitivity, relatively low cost and non-pollution advantages, especially in the detection of cancer, infectious diseases and other physiological diseases has great potential. [0003] In the process of tumor detection, the advantages of treating biological substances with freeze-drying method are outstanding, the main properties of the treated substances remain unchanged, the volatile components are less and the rehydration is good, and the activity of biological tissues is maintained to the maximum extent, which is conducive to the long-term survival of the substances. deposit. ...
Claims
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