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Plant leaf width related protein KRDP1 and a coding gene thereof and application thereof

A plant leaf and plant technology, applied in the field of plant leaf width-related protein KRDP1 and its coding gene and application, can solve problems such as insect resistance and herbicide resistance

Inactive Publication Date: 2019-06-25
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The progress and application of transgenic maize for improvement of other agronomic traits is not as ideal as that of insect resistance and herbicide resistance, mainly because there are no good trait improvement genes, and most of the agronomic traits are mainly caused by some minor effects of multi-gene control, such as the improvement of leaf width. aspect, there has been no ideal gene for manipulation

Method used

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  • Plant leaf width related protein KRDP1 and a coding gene thereof and application thereof
  • Plant leaf width related protein KRDP1 and a coding gene thereof and application thereof
  • Plant leaf width related protein KRDP1 and a coding gene thereof and application thereof

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Experimental program
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Embodiment 1

[0059] Embodiment 1, discovery of gene

[0060] The inventor of the present invention identified a pair of near-isogenic lines with obvious differences in leaf width from the natural population of the Nongda 108 recombinant inbred line. Using the mapping population constructed by the near-isogenic lines, cloned the Maize KRDP1 gene encodes the protein shown in Sequence 1 of the Sequence Listing. The cDNA of the maize inbred line X178 has the DNA molecule shown in sequence 2 of the sequence table, wherein positions 1-2088 are the open reading frame of the KRDP1 gene.

[0061] details as follows:

[0062] Corn inbred line X178 was crossed with corn inbred line Huang C to obtain F1 generation (namely Nongda 108), and Nongda 108 was self-crossed to obtain F1 generation 2 Generation, F 2 F 6:7 , obtained multiple RILs, including near-isogenic lines W964 and W968. The leaf width of RIL population was normally distributed. The segregation ratio of leaf width in BC1 population w...

Embodiment 2

[0069] Example 2. Obtaining transgenic pure line KRDP1-1 by gene editing and selfing

[0070] 1. Construction of recombinant plasmids

[0071] The basic vector adopts pCAMBIA3301 vector. The selection marker of the pCAMBIA3301 vector in bacteria is the kanamycin resistance gene, which encodes aminoglycoside phosphotransferase. There is no report that its encoded product has toxic side effects on animals. Because it is located on the periphery of the T-DNA region, it will not transfer to plant cells, so it is not pathogenic, and the probability of becoming pathogenic is extremely low. The origin of replication of the pCAMBIA3301 vector, PBR ori, comes from Escherichia coli. Its main function is to help the vector replicate in E. coli. It is widely used in the construction of engineering vectors. It only exists as an origin of replication without encoding protein, and it is located in the T-DNA The periphery of the area cannot be transferred to plant cells, so it is not pathog...

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Abstract

The invention discloses a plant leaf width related protein KRDP1 and a coding gene thereof and application thereof. The protein is named as a KRDP1 protein and is a protein as shown in a sequence 1 ina sequence table. Nucleic acid molecules encoding the KRDP1 protein are also within the protection scope of the invention. The invention also provides a method of preparing a transgenic plant. The method comprises the step of introducing a substance that inhibits expression of the nucleic acid molecules into a recipient plant to obtain a transgenic plant having a reduced leaf width. Biological functions of corn related genes are studied by focusing on the important characteristics and ultimate goals of corn yield and growth potential. On the one hand, the level of maize genetically modified breeding in China can be significantly improved, the gap with the international advanced level is narrowed, and strong technical support is provided for ensuring national food security and ecological security and improving the international competitiveness of agricultural products, which will certainly bring revolutionary changes to the development of the corn industry in China.

Description

technical field [0001] The invention belongs to the field of plant breeding, and in particular relates to a plant leaf width-related protein KRDP1, its coding gene and application. Background technique [0002] Corn is the cereal crop with the widest planting range and the largest yield in the world, ranking first among the three major grains (corn, wheat, and rice). my country is a big country in corn production and consumption. Its sown area, total output and consumption are second only to the United States, ranking second in the world. In recent years, although my country's corn has a good momentum of development, it still needs to be imported in large quantities, and the import volume is increasing rapidly year by year. With the rapid development of industrialization and urbanization and the continuous improvement of people's living standards, my country has entered a stage of rapid growth in corn consumption. From the perspective of future development, corn will be th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/12A01H6/46
Inventor 赖锦盛宋伟彬赵海铭
Owner CHINA AGRI UNIV