Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of Bangia fusco-purpurea polysaccharide in preparation of medicine for promoting wound healing

A technology for wound healing and red hair algae, applied in the field of medicine, can solve the problem that the application of red hair algae polysaccharide has no relevant literature reports and the like

Pending Publication Date: 2019-06-28
JIMEI UNIV
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports about the red hair algae polysaccharide, and there is no relevant literature report about the application of the red hair algae polysaccharide in the medicine for promoting wound healing.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of Bangia fusco-purpurea polysaccharide in preparation of medicine for promoting wound healing
  • Application of Bangia fusco-purpurea polysaccharide in preparation of medicine for promoting wound healing
  • Application of Bangia fusco-purpurea polysaccharide in preparation of medicine for promoting wound healing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The preparation of embodiment 1 red hair algae polysaccharide

[0032] The fresh red hair algae were washed and dried in an oven at 50°C to constant weight, and then pulverized into algal powder with a pulverizer. The algal powder was repeatedly soaked and washed three times by adding appropriate amount of methanol, and soaked overnight to partially remove pigment substances and alcohol-soluble impurities. After soaking, centrifuge at 4000g for 15min to collect the precipitate; add 1L of distilled water to the precipitate, extract in hot water at 90-100℃ for 2h, after extraction, centrifuge at 4000g for 20min, collect the supernatant, and repeat the residue Add 1 L of distilled water for hot water extraction at 90-100° C. for 2 h, centrifuge, combine the supernatants obtained by the two centrifugations, and evaporate and concentrate to 500 mL under reduced pressure to obtain a crude polysaccharide solution.

[0033] Mix chloroform and n-butanol in a volume ratio of 4:1...

Embodiment 2

[0035] Example 2 Determination of the toxicity of red hair algae polysaccharide to human umbilical vein endothelial cells (HUVEC) and human skin fibroblasts (HSF)

[0036] Toxicity of red algae polysaccharide to human umbilical vein endothelial cells (HUVEC) and human skin fibroblasts (HSF) was determined by MTT method.

[0037] Take the preserved HUVECs (or HSFs) for culture, and the HUVECs (or HSFs) in the logarithmic growth phase at 3 × 10 4 The concentration of cells / well was seeded in a 96-well cell culture plate at 37°C, 5% CO. 2 After incubating in the incubator for 24h, the cells were treated with different concentrations of red algae polysaccharide solution (0-500μg / mL) for 24h. After removing the supernatant, 50μL of MTT solution was added to each well, and the incubation was continued for 30min, and the supernatant was removed. After the solution, add 100 μL DMSO, shake for 20 min, and measure the absorbance at 570 nm (OD 570 ), and cell viability was calculated a...

Embodiment 3

[0041] Example 3 Experiment on the effect of red algae polysaccharide on the migration of human umbilical vein endothelial cells (HUVEC)

[0042] The preserved cells were cultured in HUVEC, and human umbilical vein endothelial cells (HUVEC) in logarithmic growth phase were grown at 5 × 10 5 The concentration of cells / well was seeded in 6-well cell culture plates at 37°C, 5% CO. 2 After 24 h of incubation in the incubator, use a sterile 200 μL plastic pipette tip to prepare a scratch of the monolayer of cells, then gently wash 1 time with PBS to remove the scraped cells. Next, human umbilical vein endothelial cells (HUVECs) were treated with 0 μg / mL, 20 μg / mL, 100 μg / mL, and 500 μg / mL of polysaccharide, respectively. Images were taken at 0 and 24 h using a bright field microscope (40x magnification). Analysis was performed using Image J and wound healing rate was calculated according to the following formula:

[0043]

[0044] Among them, T 0 is the scratched area at 0 h...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the field of medicines, in particular to a new application of Bangia fusco-purpurea polysaccharide, especially to an application of the Bangia fusco-purpurea polysaccharide inpreparation of a medicine for promoting wound healing. The results showed that the Bangia fusco-purpurea polysaccharide had no toxicity to human umbilical vein endothelial cells (HUVEC) and human skin fibroblasts (HSF), and could promote the migration and tube formation of the human umbilical vein endothelial cells (HUVEC) and obviously promote the healing of human umbilical vein endothelial cells (HUVEC) wounds.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a new application of a red algae polysaccharide in the preparation of a medicine for promoting wound healing. Background technique [0002] Medical dressing is a type of medical material used to cover damaged wounds. It protects the human body from physical, chemical, and pathogenic microorganisms, and has functions such as heat preservation, moisturizing, and immunity. At present, the researched or applied sugar-based medical dressings include chitosan and alginate. Chitosan is used in medical dressings because it is non-toxic and stimulates the growth of chondrocytes at low concentrations; alginate dressings can promote wound regeneration. Tissue regeneration, increase the speed of wound healing. Therefore, research on non-toxic substances with potential to promote wound healing is of great significance for finding reasonable measures to promote wound healing. [0003] Bangi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/715A61P17/02A61L15/28A61L15/44C08B37/00
Inventor 姜泽东何萍萍倪辉朱艳冰李清彪伍菱陈艳红杜希萍杨远帆李利君蔡慧农
Owner JIMEI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com