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Fluorescence probe for identifying cupric ions under alkaline condition and preparation method and application of fluorescence probe

A fluorescent probe, alkaline condition technology, applied in fluorescence/phosphorescence, chemical instruments and methods, luminescent materials, etc., can solve the problem that fluorescent probes cannot be used as alkaline indicators for quantitative detection, etc., and achieve resistance to other metal ions interference Strong ability, economical raw materials, good selectivity

Active Publication Date: 2019-06-28
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention is to solve the problem that the existing fluorescent probes cannot be used as basic indicators and simultaneously perform quantitative detection of copper ions under strong basic conditions, and provide a A fluorescent probe for recognizing copper ions under alkaline conditions and its preparation method and application

Method used

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  • Fluorescence probe for identifying cupric ions under alkaline condition and preparation method and application of fluorescence probe
  • Fluorescence probe for identifying cupric ions under alkaline condition and preparation method and application of fluorescence probe
  • Fluorescence probe for identifying cupric ions under alkaline condition and preparation method and application of fluorescence probe

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specific Embodiment approach 1

[0042] Specific embodiment 1: The fluorescent probe that recognizes copper ions under alkaline conditions in this embodiment is a covalent combination of 2-(2-hydroxyphenyl)benzothiazole and o-hydroxybenzoic hydrazide, and the fluorescent probe The structural formula is as follows:

[0043]

[0044] In this embodiment, the probe itself is in DMF / H 2 O(3 / 7, v / v, 0.01M HEPES) has almost no fluorescence emission in acidic to neutral solutions. When the solution is alkaline, the solution emits green fluorescence, and the fluorescence intensity increases with the alkaline degree of the solution. The experimental results show that the probe solution has almost no fluorescence emission in the pH range of 2-7. And when the pH range is 8-13, the solution has obvious blue-green fluorescence emission, and the greater the alkalinity of the solution, the stronger the fluorescence intensity visible to the naked eye. At the same time, under strong alkaline conditions, the fluorescence o...

specific Embodiment approach 2

[0047] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is that the fluorescent probe has a fluorescence detection limit of 1.35×10 for copper ions under the condition of pH=13. -6 M. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0048] Specific embodiment 3: The difference between this embodiment and specific embodiment 1 is that the detection limit of ultraviolet absorption of the fluorescent probe to copper ions is 3.75×10 under the condition of pH=13. -7 M. Others are the same as in the first embodiment.

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Abstract

The invention provides a fluorescence probe for identifying cupric ions under an alkaline condition and a preparation method and application of the fluorescence probe, and mainly solves the problem that an existing fluorescence probe cannot quantificationally detect the cupric ions under a strongly basic condition while serving as a basic indicator. The fluorescence probe is a covalent combinationof 2-(2-hydroxyphenyl)benzothiazole and salicylhydrazide. The method comprises the steps that 1, 3-methylsalicylaldehyde and o-aminobenzenethiol react to obtain a compound 1; 2, the compound 1 obtained in step 1 reacts with urotropine to obtain a compound 2; 3, the compound 2 obtained in step 2 reacts with the salicylhydrazide to obtain a target compound Z. The fluorescence probe is used for thesensing detection of the content of the cupric ions under the strongly basic condition, and is used for the detection of pH values as a weakly basic indicator. The invention belongs to the field of fluorescence probes.

Description

technical field [0001] The invention relates to a fluorescent probe for recognizing copper ions, a preparation method and application thereof. Background technique [0002] The stability of the pH value in the human body is an important factor to maintain the normal physiological functions of life. If the pH in the living body is abnormal, it will lead to the disorder of the normal functions in the human body, which in turn will cause some physiological diseases, such as stroke, Alzheimer's disease, heart and brain Vascular disease and even cancer. In eukaryotic cells, organelles correspond to different pH values. For example, the pH in the cytoplasm is slightly alkaline, while the lysosome is slightly acidic (pH about 4.0-6.0). Under acidic pH conditions, the hydrolytic enzymes in lysosomes (such as protease, phosphatase, lipase, nuclease, etc.) will be activated, and the stable pH ensures the normal function of lysosomes. In addition, in water quality detection, pH is an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D277/66C09K11/06G01N21/33G01N21/64G01N21/80
Inventor 邢志勇曾爽李明强刘程国
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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