Edible packaging film with dual protein as substrate and preparation method of edible packaging film
A double-protein, packaging film technology, applied in fermentation and other directions, can solve problems such as coagulation, egg instability, packaging film mechanical properties, poor barrier properties, etc., and achieve the effect of uniform thickness, good appearance quality, and fast degradation speed
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Embodiment 1
[0023] (1) Remove the shell of the egg, beat the egg yolk and egg white evenly to obtain the whole egg liquid, take 15g of the whole egg liquid and add 50ml of distilled water, adjust the pH to 8.0 with 1mol / L NaOH, add 2500U / g (substrate) trypsin and 2500U / g (substrate) alkaline protease;
[0024] (2) After the enzymolysis starts, the enzymolysis system is ultrasonically treated, and the treatment conditions are frequency 20kHz, power 225W, and temperature 50°C. Ultrasonic treatment adopts intermittent ultrasonic treatment, and ultrasonic treatment is carried out for 3 minutes at the 0th, 20th and 40th minutes of enzymatic hydrolysis, and the enzymatic hydrolysis time is 1 hour;
[0025] (3) Add 250ml of distilled water at a temperature of 40-60°C in the beaker, add 15g of soybean protein isolate in terms of weight percentage of distilled water, mix with the whole egg liquid obtained through pretreatment, add plasticizer and crosslinking agent, Obtain a mixed solution after ...
Embodiment 2
[0030] (1) Remove the shell of the egg, beat the egg yolk and egg white evenly to obtain the whole egg liquid, take 15g of the whole egg liquid and add 50ml of distilled water, adjust the pH to 8.0 with 1mol / L NaOH, add 3000U / g (substrate) trypsin and 2000U / g (substrate) alkaline protease;
[0031] (2) After the enzymolysis starts, the enzymolysis system is ultrasonically treated, and the treatment conditions are frequency 20kHz, power 225W, and temperature 50°C. Ultrasonic treatment adopts intermittent ultrasonic treatment, and ultrasonic treatment is carried out for 3 minutes at the 0th, 20th and 40th minutes of enzymatic hydrolysis, and the enzymatic hydrolysis time is 1 hour;
[0032] (3) Add 250ml of distilled water at a temperature of 40-60°C in the beaker, add 15g of soybean protein isolate in terms of weight percentage of distilled water, mix with the whole egg liquid obtained through pretreatment, add plasticizer and crosslinking agent, Obtain a mixed solution after ...
Embodiment 3
[0037] (1) Shell the eggs, beat the egg yolks and egg whites to obtain whole egg liquid, take 15g whole egg liquid and add 50ml distilled water, adjust the pH to 8.0 with 1mol / L NaOH, add 2000U / g (substrate) trypsin and 3000U / g (substrate) alkaline protease;
[0038] (2) After the enzymolysis starts, the enzymolysis system is ultrasonically treated, and the treatment conditions are frequency 20kHz, power 225W, and temperature 50°C. Ultrasonic treatment adopts intermittent ultrasonic treatment, and ultrasonic treatment is carried out for 3 minutes at the 0th, 20th and 40th minutes of enzymatic hydrolysis, and the enzymatic hydrolysis time is 1 hour;
[0039] (3) Add 250ml of distilled water at a temperature of 40-60°C in the beaker, add 15g of soybean protein isolate in terms of weight percentage of distilled water, mix with the whole egg liquid obtained through pretreatment, add plasticizer and crosslinking agent, A mixed solution is obtained after magnetic stirring;
[0040...
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