Bacillus megaterium P5-2 and separation method and application thereof

A technique for the isolation of Bacillus megaterium, applied in the field of Bacillus megaterium P5-2 and its isolation, can solve the problems of weak environmental adaptability, slow growth of strains, and low denitrification efficiency, and achieve strong environmental adaptability and growth Fast speed and high nitrogen removal efficiency

Active Publication Date: 2019-06-28
PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, in the practical application of microbial nitrogen removal method, because it is an oligotrophic condition, the gro

Method used

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  • Bacillus megaterium P5-2 and separation method and application thereof
  • Bacillus megaterium P5-2 and separation method and application thereof
  • Bacillus megaterium P5-2 and separation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0029] Example 1

[0030] Isolation of Bacillus megaterium P5-2

[0031] S1, sampling

[0032] In the Tilapia Factory Recirculating Aquaculture Workshop of Panyu Fine Breeding Farm in Guangzhou City, aquaculture water samples and biological fillers are taken and stored in a refrigerator at 4°C for enrichment and separation;

[0033] S2, preliminary screening

[0034] Add 120mL of No. 5 enrichment and separation medium in a 250ml Erlenmeyer flask, use a 100mL graduated cylinder to take 20mL water sample and add it to a 50mL centrifuge tube, shake for about 30s, and put it into No. 5 enrichment and separation solid medium (containing 15 biological Filler), placed in a shaker at 32°C and 180r / min for 5-7 days, repeat the above operation twice, take the third enriched culture solution and dilute it to 10 -3 , 10 -4 , 10 -5 , Take 150μL each of the dilutions, apply to No. 5 enriched and separate solid medium, make 2 dishes for each dilution concentration, and place them in a constant temper...

Example Embodiment

[0045] Example 2

[0046] The isolated and purified Bacillus megaterium (Bacillus megaterium) P5-2 with high efficient denitrification, the colony morphology on LB solid medium is like figure 1 As shown, the Gram stain is positive ( figure 2 ); Hydrolyzable starch ( Figure 3-1 ) And casein ( Figure 3-2 );

Example Embodiment

[0047] Example 3

[0048] The safety of strain P5-2 to tilapia was tested by intraperitoneal injection and soaking. The results of the injection experiment showed that the concentration of 100μL injection of tilapia with a body weight of (6.5±1.5)g was 1.5×10 7 CFU / ml, 1.5×10 8 CFU / ml and 1.5×10 9 Under the condition of CFU / mL bacterial liquid, there was no death or other abnormal conditions; the immersion experiment results showed that the concentration of P5-2 in the water body was 1.5×10 6 CFU / mL and 1.5×10 7 In the case of CFU / mL, tilapia weighing (1.0±0.5)g did not die first and other abnormal phenomena. The above results indicate that P5-2 is a strain with good safety performance.

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Abstract

The invention belongs to the field of microorganism application, and specifically relates to a strain of bacillus megaterium P5-2 and a separation method and application thereof. The bacillus megaterium P5-2 is preserved in the Guangdong Microbial Culture Collection Center on January 7th, 2019, and the preservation number is GDMCC NO.60536. The bacillus megaterium is high in growth speed, denitrification efficiency and environmental adaptability and has no damage to aquatic products, the ammonia nitrogen removal rate under oligotrophic conditions is 60% or above, and ammonia nitrogen in waterand nitrite nitrogen can both be removed. When the bacillus megaterium is used as a nitrogen removal agent, since the bacillus megaterium has no damage to the aquatic products, the bacillus megateriumis particularly suitable for removing nitrogen from the aquaculture water.

Description

technical field [0001] The invention belongs to the field of microbes, and in particular relates to a strain of bacillus megaterium P5-2 and its isolation method and application. Background technique [0002] Microbial denitrification technology is the use of microorganisms to remove NO in sewage under artificial control. 3- Revert to N 2 The process of removing nitrate nitrogen from wastewater. If the nitrogen-containing compound in the wastewater is ammonia nitrogen, the ammonia nitrogen is first oxidized to nitrate nitrogen, that is, nitrification is performed first, and then denitrification is performed under anaerobic conditions to carry out denitrification, forming a "nitrification-denitrification process". Nitrification" denitrification process and technology. [0003] However, in the practical application of the microbial nitrogen removal method, because it is an oligotrophic condition, the growth rate of the strain used is slow, the nitrogen removal efficiency is...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02C02F3/34C12R1/11
Inventor 卢迈新李忠徽王淼衣萌萌刘志刚高风英曹建萌可小丽
Owner PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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