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Bacillus megaterium P5-2 and separation method and application thereof

A technique for the isolation of Bacillus megaterium, applied in the field of Bacillus megaterium P5-2 and its isolation, can solve the problems of weak environmental adaptability, slow growth of strains, and low denitrification efficiency, and achieve strong environmental adaptability and growth Fast speed and high nitrogen removal efficiency

Active Publication Date: 2019-06-28
PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, in the practical application of microbial nitrogen removal method, because it is an oligotrophic condition, the growth rate of the strain used is slow, the nitrogen removal efficiency is low, and the environmental adaptability is relatively weak.

Method used

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  • Bacillus megaterium P5-2 and separation method and application thereof
  • Bacillus megaterium P5-2 and separation method and application thereof
  • Bacillus megaterium P5-2 and separation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Isolation of Bacillus megaterium P5-2

[0031] S1, sampling

[0032] In the tilapia industrialized recirculating aquaculture workshop of the Panyu Fine Breed Farm in Guangzhou City, water samples of the aquaculture water and biological fillers were taken and stored in a refrigerator at 4°C for enrichment and separation;

[0033] S2, primary screening

[0034] Add 120mL of No. 5 enrichment and separation medium to a 250ml Erlenmeyer flask, use a 100mL graduated cylinder to take 20mL of water sample, add it to a 50mL centrifuge tube and shake for about 30s, then put it into No. 5 enrichment and separation solid medium (containing 15 biological Filler), placed in a shaker at 32°C and 180r / min for constant temperature cultivation for 5-7 days, repeated the above operation twice, and took the third enriched culture solution for gradient dilution and diluted it to 10 -3 、10 -4 、10 -5 , take 150 μL each of the dilutions, spread them on No. 5 enrichment and separation solid...

Embodiment 2

[0046] The isolated and purified heterotrophic Bacillus megaterium (Bacillus megaterium) P5-2 with high denitrification efficiency, the colony morphology on LB solid medium is as follows: figure 1 As shown, the Gram stain was positive ( figure 2 ); hydrolyzable starch ( Figure 3-1 ) and casein ( Figure 3-2 );

Embodiment 3

[0048] The safety of strain P5-2 to tilapia was tested by intraperitoneal injection and soaking. The results of the injection experiment showed that the concentrations of 1.5×10 7 CFU / ml, 1.5×10 8 CFU / ml and 1.5×10 9 Under the condition of CFU / mL bacterial liquid, there was no death or other abnormal conditions; the results of immersion test showed that the concentration of P5-2 in water was 1.5×10 6 CFU / mL and 1.5×10 7 In the case of CFU / mL, tilapia with body weight of (1.0±0.5) g did not appear premature death and other abnormal phenomena. The above results show that P5-2 is a strain with good safety performance.

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Abstract

The invention belongs to the field of microorganism application, and specifically relates to a strain of bacillus megaterium P5-2 and a separation method and application thereof. The bacillus megaterium P5-2 is preserved in the Guangdong Microbial Culture Collection Center on January 7th, 2019, and the preservation number is GDMCC NO.60536. The bacillus megaterium is high in growth speed, denitrification efficiency and environmental adaptability and has no damage to aquatic products, the ammonia nitrogen removal rate under oligotrophic conditions is 60% or above, and ammonia nitrogen in waterand nitrite nitrogen can both be removed. When the bacillus megaterium is used as a nitrogen removal agent, since the bacillus megaterium has no damage to the aquatic products, the bacillus megateriumis particularly suitable for removing nitrogen from the aquaculture water.

Description

technical field [0001] The invention belongs to the field of microbes, and in particular relates to a strain of bacillus megaterium P5-2 and its isolation method and application. Background technique [0002] Microbial denitrification technology is the use of microorganisms to remove NO in sewage under artificial control. 3- Revert to N 2 The process of removing nitrate nitrogen from wastewater. If the nitrogen-containing compound in the wastewater is ammonia nitrogen, the ammonia nitrogen is first oxidized to nitrate nitrogen, that is, nitrification is performed first, and then denitrification is performed under anaerobic conditions to carry out denitrification, forming a "nitrification-denitrification process". Nitrification" denitrification process and technology. [0003] However, in the practical application of the microbial nitrogen removal method, because it is an oligotrophic condition, the growth rate of the strain used is slow, the nitrogen removal efficiency is...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02C02F3/34C12R1/11
Inventor 卢迈新李忠徽王淼衣萌萌刘志刚高风英曹建萌可小丽
Owner PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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