Seedling raising method of gerbera jamesonii haploid transplanted seedlings
A haploid and gerbera technology, applied in the field of plant biotechnology and cultivation, can solve the problems of low survival rate of gerbera haploid transplantation, and achieve the effects of reducing drug dosage, improving quality and saving costs
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Embodiment 1
[0034] A method for raising seedlings of gerbera haploid transplanted seedlings, comprising the following steps:
[0035] A. Subculture
[0036] The young shoots of gerbera haploid were excised and transferred to a culture bottle filled with the following proliferation medium for subculture:
[0037] MS medium
[0038]
[0039]
[0040] The subculture conditions of the culture bottle in the culture room are as follows: light intensity 1500-2000lx, temperature 25°C±2°C, light time 10h / d, after 25 days of subculture, transfer to a new container containing the same proliferation culture as above. In the culture bottle of culture medium, and continue to subculture 25d under above-mentioned same subculture condition, repeat like this;
[0041] B. Cultivation of strong seedlings
[0042] When the number of multiplication bases for subculture in step A reaches the required number, transfer to a culture bottle filled with the following strong seedling medium for strong seedli...
Embodiment 2
[0058] Embodiment 2 is the same as Embodiment 1 except for the following operations, and will not be repeated here.
[0059] B. Cultivation of strong seedlings
[0060] In the strong seedling medium: the concentration of kinetin (KT) is 0.05mg / L;
[0061] The light time is 12h / d, and the cultivation time for strong seedlings is 25d;
[0062] C. Rooting culture
[0063] In rooting medium: the concentration of indole butyric acid (IBA) is 0.4mg / L.
Embodiment 3
[0065] Embodiment 3 is the same as Embodiment 1 except for the following operations, and will not be repeated here.
[0066] B. Cultivation of strong seedlings
[0067] In the strong seedling medium: the concentration of kinetin (KT) is 0.1mg / L;
[0068] The light time is 11h / d, and the cultivation time for strong seedlings is 30d;
[0069] C. Rooting culture
[0070] In the rooting medium: the concentration of indole butyric acid (IBA) is 0.5mg / L.
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