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Method for separating functional nanoparticles in aquatic shellfish cooked soup

A separation method and nanoparticle technology, applied in separation methods, filtration separation, chemical instruments and methods, etc., can solve problems such as protein denaturation and loss of active components, time-consuming size exclusion chromatography separation, and inapplicability to general sample preservation. , to achieve the effect of mild separation, high yield and convenient operation

Active Publication Date: 2019-07-05
ZHEJIANG GONGSHANG UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For example, the sensitivity of static multi-angle laser light scattering is low, the cost is relatively expensive, and the separation cost is high; the separation time of size exclusion chromatography is long, and the separation efficiency is low; the high salt elution process of ion exchange chromatography may affect the The system causes certain interference, and the higher ionic strength can easily cause the denaturation of some proteins and the loss of active ingredients, and may not be suitable for the preservation of general samples

Method used

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  • Method for separating functional nanoparticles in aquatic shellfish cooked soup
  • Method for separating functional nanoparticles in aquatic shellfish cooked soup
  • Method for separating functional nanoparticles in aquatic shellfish cooked soup

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Wash the clams with distilled water and rest for 5 hours, weigh live fresh individuals, put them into a stainless steel pot containing distilled water according to the ratio of material to liquid 1kg: 1L, boil on an induction cooker for 1 hour, cool and filter with gauze;

[0033] (2) Take the crude filtrate in a centrifuge at a centrifugation speed of 15000r·min-1, and centrifuge at 4°C for 15min to obtain a supernatant;

[0034] (3) The supernatant is quickly separated by a gel exclusion chromatography column (10mm×120mm). The column filler is SephoraseCL-4B. The column is filled with 0.05M phosphate buffer (pH7.2) and used for equilibrium. The flow rate is: 0.5mL / min, the sample volume is 1mL, and the peak of the functional nanoparticles is detected by the ultraviolet band 280nm combined with the online dynamic laser light scattering instrument (SEC-DLS) (see figure 1 ), which is different from free protein components. Collect in different tubes, each tube is 2...

Embodiment 2

[0035] Example 2: SEC-DLS can continuously separate the nanoparticles in the clam soup and detect the nanometer size on-line, and the nanoparticle population of 150-40nm can be obtained, and the resolution can reach 1nm. Separation spectrum see figure 1 , the particle morphology see figure 2 , figure 2 The comparative reference Soup in refers to the supernatant.

Embodiment 3

[0036] Example 3: Compared with the original soup particles, the size of the nanoparticles obtained by SEC does not increase or decrease sharply, indicating that the SEC separation conditions are relatively mild. See you separately image 3 and Table 1.

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Abstract

The invention discloses a method for separating functional nanoparticles in an aquatic shellfish cooked soup. The separation method comprises the following steps: heating aquatic shellfish to preparethe aquatic shellfish cooked soup; centrifuging the aquatic shellfish cooking soup to obtain a supernatant; and separating the supernatant by a gel exclusion chromatograph combined dynamic light scattering instrument to obtain the functional nanoparticles in aquatic shellfish cooked soup. The nanoparticles can effectively load active ingredients such as taurine, ornithine and phytosterol, and canbe used in medicines or foods for regulating body immunity and treating liver diseases. The method has the advantages of rapidness in separation, high yield, no damages to a sample, high resolution, safe and green process, and wide application range.

Description

technical field [0001] The invention belongs to the technical field of food and nanometer materials, in particular to a method for separating functional nanoparticles in aquatic shellfish cooking soup. Background technique [0002] Aquatic shellfish, such as the river clam (Corbicula fluminea) is a bivalve mollusk with a shell that is yellow-green, brown-yellow, or dark brown, and belongs to the different species of Eulamellibranchia in the class Lamellibranchia. The genus Corbicula in the family Corbiculidae of the suborder Heterodontta, also known as yellow clams, golden clams, flat snails, etc. River clams are one of the important economic shellfish in my country, widely distributed in warm and humid freshwater or brackish water rivers and lakes, such as Zhejiang, Fujian, Jiangsu, Taiwan and other provinces in the southeast. The soft body part (clam meat) of river clam is not only delicious, but also has quite significant health value. [0003] Nanomaterials, such as li...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D15/34B01D15/42B01D36/00
CPCB01D15/34B01D15/424B01D36/00
Inventor 余兆硕柯李晶高观祯汪惠勤周建武饶平凡彭彰文
Owner ZHEJIANG GONGSHANG UNIVERSITY
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