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Heme peptide iron product and preparation method thereof

A technology of heme peptide and heme, applied in the fields of biotechnology and modern agriculture, can solve the problems of high price of metal chelates, narrow application range, specific biological activity, etc.

Pending Publication Date: 2019-07-09
SHANGHAI TRANSGENIC RES CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And because the metal chelate compound of single amino acid as raw material is expensive and biologically active, the application range is narrow, which is not conducive to widespread application.

Method used

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  • Heme peptide iron product and preparation method thereof
  • Heme peptide iron product and preparation method thereof
  • Heme peptide iron product and preparation method thereof

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preparation example Construction

[0038] The preparation method of the heme peptide iron product provided by the present invention uses blood cells obtained by anticoagulation and separation of fresh healthy animal blood as raw materials, and adopts techniques such as enzymatic hydrolysis, chelation, and drying to obtain the heme peptide iron product.

[0039] Specifically, including steps:

[0040] (a) collecting animal blood cells;

[0041] (b) performing enzymatic hydrolysis on the blood cells obtained in step (a) to obtain an enzymatic hydrolysis solution;

[0042] (c) adding divalent iron ions to the enzymolysis solution obtained in step (b) for chelation treatment to obtain a heme peptide iron solution;

[0043] (d) drying the heme peptide iron solution obtained in step (c), thereby obtaining a heme peptide iron product.

[0044] combine figure 1 And preferred embodiment, further set forth the method described in the present invention:

[0045] (a) collecting animal blood cells;

[0046] (b) adjust ...

Embodiment 1

[0059] Preparation of porcine heme peptide iron product 1

[0060] (1) Separating porcine anticoagulant whole blood through a tubular centrifuge (11000 rpm), the resulting light liquid is plasma liquid, and the heavy liquid is blood cell liquid, which is used to prepare porcine heme peptide iron.

[0061] (2) Add 10.0 tons of water to 5.08 tons of the blood cell solution prepared in step (1) and mix and stir, adjust the pH to 10.0 with sodium hydroxide, add 50 kg of alkaline protease and stir for 10 hours at 58° C. to obtain the enzymatic solution;

[0062] (3) Add 1.0 tons of FeCl to the enzymolysis solution 2 4H 2 O stirring and chelating for 3h to obtain a heme peptide iron solution;

[0063] (4) Spray-dry the obtained solution, control the air inlet temperature to 220-240° C., and the air outlet temperature to 90-110° C., so as to obtain the heme peptide iron product.

[0064] As a result, using the above method, 5.08 tons of pig blood cells obtained 2.164 tons of heme pe...

Embodiment 2

[0068] Preparation of porcine heme peptide iron product 2

[0069] (1) Separating porcine anticoagulant whole blood through a tubular centrifuge (11000 rpm), the resulting light liquid is plasma liquid, and the heavy liquid is blood cell liquid, which is used to prepare porcine heme peptide iron.

[0070] (2) Add 12.0 tons of water to 5.20 tons of the blood cell solution prepared in step (1) and mix and stir, adjust the pH to 8.5 with sodium hydroxide, add 75 kg of neutral protease and stir for 8 hours at 50°C to obtain an enzymatic solution;

[0071] (3) Add 0.9 tons of FeCl to the enzymolysis solution 2 4H 2 O stirring and chelating for 3h to obtain a heme peptide iron solution;

[0072] (4) Spray-dry the obtained solution, control the air inlet temperature to 230-250° C., and the air outlet temperature to 90-110° C., so as to obtain the heme peptide iron product.

[0073] As a result, using the above method, 5.20 tons of pig blood cells obtained 2.136 tons of heme peptid...

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Abstract

The invention relates to a heme peptide iron product and a preparation method thereof. The preparation method includes subjecting blood cell liquid obtained by anticoagulation and separation of animalblood to enzymolysis so as to obtain enzymolysis liquid, adding divalent iron salt into the enzymolysis liquid for chelation reaction, and subjecting the reaction liquid to spray drying so as to obtain the heme peptide iron product. The heme peptide iron product is rich in heme and oligopeptide, high in iron chelation rate, stable in quality, low in cost and suitable for large-scale production.

Description

technical field [0001] The invention relates to the fields of biotechnology and modern agriculture, in particular to a preparation method and application of heme peptide iron. Background technique [0002] Iron deficiency anemia is one of the main causes of serious impact on human health worldwide, especially in countries where plant food is the staple food. At present, the prevention and treatment of iron deficiency anemia is mainly achieved by taking iron supplements. Common iron supplements include ferrous sulfate, ferrous chloride, etc., but their bioavailability in the body is low, and there are obvious toxic and side effects . Heme peptide iron is a biological state iron formed by the heme polypeptide produced by the hydrolysis of animal blood cells and chelated with ferrous ions. It has the advantages of good stability, high absorption rate, and low toxicity. It is an ideal iron supplement agent. [0003] In developed countries such as the United States, Japan, and...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K14/805C07K1/107A61K38/42A61P7/06
CPCC12P21/06C07K14/805A23K50/30A23K50/60A23K20/30A23K20/147A23L33/165A23L33/18A61K38/00A23V2002/00A23V2200/214A23V2200/30A23V2250/1592A23V2250/55
Inventor 成国祥江国永朱逸欣吕小东
Owner SHANGHAI TRANSGENIC RES CENT
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