Fluorescent sensor for efficiently detecting diethylamine and preparation method and application thereof
A fluorescence sensor and diethylamine technology, applied in the field of fluorescence analysis and detection, can solve the problem of single efficient selective detection, and achieve the effects of good selectivity, fast response speed and good stability
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Embodiment 1
[0029] Based on hollow silica particle photonic crystal (band gap wavelength is 520nm), 10 -5 mol / L Rhodamine 6G is a fluorescent dye, which can detect 5.0mg / L diethylamine.
[0030] Step 1: Prepare the fluorescent probe solution, dissolve the fluorescent dye in ethanol, mix evenly with ultrasound, and set the volume to a concentration of 10 -5 mol / L;
[0031] Step 2: Infiltrate the fluorescent probe solution obtained in Step 1 into the hollow spherical photonic crystal substrate, and cover the surface with a plexiglass sheet of the same size to form a "sandwich" structure of glass / photonic crystal substrate / glass.
[0032] The detection steps are as follows:
[0033] Place the resulting "sandwich" structure of glass / photonic crystal substrate / glass horizontally, record the initial fluorescence emission spectrum under the irradiation of 365nm excitation light, then add 5.0mg / L diethylamine dropwise, observe the fluorescence enhancement effect, and record the fluorescence emi...
Embodiment 2
[0036] Based on hollow silica particle photonic crystal (band gap wavelength is 520nm), 10 -5 mol / L rhodamine 6G is a fluorescent dye, which can detect 0.5mg / L diethylamine.
[0037] Step 1: Prepare the fluorescent probe solution, dissolve the fluorescent dye in ethanol, mix evenly with ultrasound, and set the volume to a concentration of 10 -5 mol / L;
[0038] Step 2: Infiltrate the fluorescent probe solution obtained in Step 1 into the hollow spherical photonic crystal substrate, and cover the surface with a plexiglass sheet of the same size to form a "sandwich" structure of glass / photonic crystal substrate / glass.
[0039] The detection steps are as follows:
[0040] Place the resulting "sandwich" structure of glass / photonic crystal substrate / glass horizontally, record the initial fluorescence emission spectrum under the irradiation of 365nm excitation light, then add 0.5mg / L diethylamine dropwise, observe the fluorescence enhancement effect, and record the fluorescence emi...
Embodiment 3
[0043] Based on hollow silica particle photonic crystal (band gap wavelength is 520nm), 10 -5 mol / L rhodamine 6G is a fluorescent dye, and can detect 0.05mg / L diethylamine.
[0044] Step 1: Prepare the fluorescent probe solution, dissolve the fluorescent dye in ethanol, mix evenly with ultrasound, and set the volume to a concentration of 10 -5 mol / L;
[0045] Step 2: Infiltrate the fluorescent probe solution obtained in Step 1 into the hollow spherical photonic crystal substrate, and cover the surface with a plexiglass sheet of the same size to form a "sandwich" structure of glass / photonic crystal substrate / glass.
[0046] The detection steps are as follows:
[0047] Place the resulting "sandwich" structure of glass / photonic crystal substrate / glass horizontally, record the initial fluorescence emission spectrum under the irradiation of 365nm excitation light, then add 0.05mg / L diethylamine dropwise, observe the fluorescence enhancement effect, and record the fluorescence emi...
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