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Gram staining method

A Gram staining, Gram-positive technology, applied in the field of Gram staining, can solve the problems of bacterial deformation, influence, infection risk, etc., achieve powerful result judgment, improve departmental safety management methods, and simple operation Effect

Pending Publication Date: 2019-07-16
JILIN JINYU MEDICAL SCI INSPECTION CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method of operation has a certain risk of infection for the operator and the operating environment. In addition, the use of flame heating and fixing, uneven heating of the bacteria can easily cause deformation of the bacteria, which affects the judgment of the operator on the experimental results

Method used

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Examples

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Effect test

Embodiment 1

[0024] The specific operation steps of the Gram staining method provided by the present invention are as follows: Take 9 glass slides and divide them into three groups. Each slide is wiped dry with gauze, and a small amount of Gram-positive cocci (Golden yellow grape Coccus) spread on each glass slide to make it thin and even. After the slide is finished, put it directly on the desktop of the biological safety cabinet, turn on the ultraviolet light and irradiate it for 20, 30, and 40 minutes. Dyeing can be checked by microscope. The dyeing method is: dye with ammonium oxalate crystal violet for 1 minute; rinse with distilled water; cover the coated surface with iodine solution and dye for about 1 minute; wash with water and absorb water with absorbent paper; add a few drops of 95% alcohol and lightly Shake gently to decolorize, wash with water after 20 seconds to absorb water; rinse with saffron staining solution (dilute) for 1 minute, rinse with distilled water; dry, and inspec...

Embodiment 2

[0028] The Gram staining method provided by the present invention is the same as in Example 1. The specific operation steps are as follows: Take 9 slides and divide them into three groups. Each slide is wiped dry with gauze, and a small amount of Gram negative Spread the bacillus (Pseudomonas aeruginosa) on each slide to make it thin and even. After the slide is applied, place it directly on the desktop of the biological safety cabinet, and turn on the three sets of UV lamps to irradiate for 20, 30, and 40 minutes respectively. Gram staining and microscopic examination can be performed by using the existing technology. The microscopic examination results show that the three groups of bacteria are clearly visible and red, and the effect is good. The microscopic examination results of the bacteria after 40 minutes of irradiation are shown image 3 .

Embodiment 3

[0032] The Gram staining method provided by the present invention is the same as in Example 1. The specific operation steps are as follows: Take 9 slides and divide them into three groups. Each slide is wiped dry with gauze, and a small amount of Gram-positive is removed. Spores (candida albicans) are applied to each slide to make it thin and even. After the slide is applied, put it directly on the desktop of the biological safety cabinet and turn on the three sets of UV lamps to irradiate for 20, 30, and 40 minutes. After time, the existing technology can be used for gram staining and microscopic examination. The microscopic examination results show that the three groups of bacteria are clearly visible and red, and the effect is good. The microscopic examination results of the bacteria after 30 minutes of irradiation are shown Figure 5 .

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Abstract

The invention discloses a Gram staining method, belongs to the technical field of microbial detection and solves the problems that the prior art has a risk of bacterial infection, unevenness of fixedforce and the like. The gram staining method comprises the steps of smearing, fixing and staining; after smearing, performing ultraviolet irradiation directly for fixing, then staining, and fully exposing bacteria thallus under ultraviolet; while killing the thallus, fixing the smeared piece, not only reducing the risk of bacterial infection, but also fixing and forcing the thallus consistently, resulting in no bacteria deformation due to uneven heating in the fixed steps of conventional heating, and affecting the result judgment of experimenters. By use of the method, staining results are more stable, result judgment is more powerful, and the method provides reliable basis for guide clinical diagnosis and treatment of infectious diseases.

Description

Technical field [0001] The invention relates to the technical field of microbial detection, in particular to a Gram staining method. Background technique [0002] Gram staining is a differential staining method widely used in bacteriology (Gramstain). This staining method was developed by a Danish doctor Hans Christian Gram (1853-1938) Invented in 1884, it was originally used to identify the relationship between pneumococcus and Klebsiella pneumoniae. For more than 100 years, the four-step method invented by Gram has been used, and it is one of the indispensable bacteria test techniques that are often used in various bacterial experiments. The test principle is that after initial crystal violet staining and iodine solution mordant staining, a water-insoluble crystal violet and iodine complex is formed in the cell wall. Gram-positive bacteria have thick cell walls, multiple layers of peptidoglycan network and dense cross-linking. , Does not contain lipids, so the mesh is reduced...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04
CPCC12Q1/04
Inventor 杨丽英周婷婷李雨艳岳志敏齐春林
Owner JILIN JINYU MEDICAL SCI INSPECTION CO LTD
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