Screening method of stress-resistance portunus trituberculatus

A technology of portunus trituratus and stress resistance, which is applied in the directions of biochemical equipment and methods, microbial determination/inspection, measuring device, etc., can solve problems such as restricting the development of the swimming crab aquaculture industry, outbreak of epidemic diseases, deterioration, etc.

Inactive Publication Date: 2019-07-19
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the gradual expansion of aquaculture density, the aquaculture water environment deteriorated, which led to a sharp decline in crab physique, and then outbreaks of epidemic diseases. The annual loss due to disease accounted for 30% to 50% of the aquaculture output. In special years, the loss in 60% to 70%, which greatly restricts the development of Portunus trituberculat

Method used

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  • Screening method of stress-resistance portunus trituberculatus
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  • Screening method of stress-resistance portunus trituberculatus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] In the experiment, 5 Portunus trituberculatus with the same development level and similar weight were selected to compare and analyze blood indicators with Portunus trituberculatus that had survived the stress resistance screening test and had good vitality. The specific steps were as follows:

[0033] 1) Morphological and subpopulation analysis of blood cells: after cell counting of the obtained cell suspension (the number of cells is controlled at 1-2x10 6 cells / mL) for flow cytometry loading. The abscissa is FSG, and the ordinate is SSG. Collect 10,000 cells to detect cell subpopulations of Portunus trituberculatus

[0034] 2) Detection of phagocytosis of blood cells: add appropriate amount of 2 μm fluorescent microspheres to the collected hemolymph above, and incubate with shaking at room temperature for 30 minutes in the dark. After the incubation is complete, wash gently twice with PBS to remove excess unbound beads. Fluorescent signals were received at the wave...

Embodiment 2

[0039] Select Portunus trituberculatus with good stress resistance in Example 1 to carry out seedling cultivation, and its parental offspring are compared with randomly selected cultured Portunus trituberculatus with unknown stress resistance, and each of the two types of crabs is selected 5

[0040] Simulate the conditions of pond culture and infect the experimental crabs with bacteria. After a week of temporary breeding, it was found that the 5 parental offspring of Portunus trituberculatus with good stress resistance still had good vitality, and the test results of their blood immune characteristics All were within the proposed standard range, while two of the other five randomly selected Portunus trituberculatus had vital signs, but did not have good vitality, and their blood cell immune characteristic test results were slightly lower than the standard, and the dead The test results of the immune characteristics of blood cells in three Portunus trituberculatus were lower th...

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Abstract

The invention aims to provide a screening method of stress-resistance portunus trituberculatus. Portunus trituberculatus good in stress resistance can be effectively screened for culturing young portunus trituberculatus. The screening method comprises the step of firstly providing a standard for screening stress-resistance portunus trituberculatus, wherein the standard of the stress-resistance portunus trituberculatus includes the following one or several indexes that the content of granulosa cells in blood cells is 38.47+/-1.78%, the content of clear cells in the blood cells is 57.44+/-1.28%,the content of active oxygen of the clear cells in the blood cells is not less than 22.7%, the content of active oxygen of the granulosa cells in the blood cells is not less than 40.19%, the phagocytosing rate of the clear cells in the blood cells is not less than 4.67%, the phagocytosing rate of the granulosa cells in the blood cells is not less than 15.29%, and the above standard is used for screening the stress-resistance portunus trituberculatus. According to the screening method disclosed by the invention, the condition for detecting the blood cells of the portunus trituberculatus with aflow cytometer is determined, the standard for screening the stress-resistance portunus trituberculatus is obtained, and the standard can be used for screening portunus trituberculatus varieties having good stress resistance.

Description

technical field [0001] The invention belongs to the technical field of detection of immune characteristics of seawater shellfish, and in particular relates to a screening method for evaluating stress resistance of Portunus trituberculatus. Background technique [0002] Portunus trituberculatus, also known as sea crab, sea crab, and gun crab, is a species of Crustacea, Decapoda, Portunidae, and Portunus. Due to the lack of a specific immune system, crustaceans must completely rely on non-specific immunity to resist the invasion of various pathogens and foreign bodies. Cell-mediated immune defense in crustaceans is primarily carried out by circulating blood cells, which are continuously produced by self-renewing hematopoietic stem cells in adult animals. Hemocytes eliminate invading pathogens through phagocytosis, encapsulation, melanization, and cell agglutination, and play a key role in the defense mechanism of crustaceans. [0003] Portunus trituberculatus farming is one ...

Claims

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Application Information

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IPC IPC(8): C12Q1/06C12Q1/04C12Q1/02
CPCG01N15/1434G01N2015/1006G01N2015/0065G01N33/5094G01N33/5005Y02A40/81
Inventor 李桂仙张春丹母昌考周君
Owner NINGBO UNIV
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