Sectional zebra fish breeding method

A culture method and staged technology, applied in the field of staged culture of zebrafish, can solve the problems of difficulty in further increasing the culture density, low utilization rate of pure oxygen, inconvenient culture operation, etc., and achieve simple and convenient water changing method, low mortality rate, The effect of high survival rate

Active Publication Date: 2019-07-30
CHINESE RES ACAD OF ENVIRONMENTAL SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] A Chinese invention patent (CN201410158549.3 A high-density fish farming method) relates to a high-density fish farming method, including the following steps: 1) feeding fry, 2) fish pond sealing, 3) water flow regulation, 4) feeding feed, 5) Drainage and sewage discharge, 6) Fishing operation, although it can solve the problems that the breeding density is difficult to further increase, the oxygenation equipme

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  • Sectional zebra fish breeding method
  • Sectional zebra fish breeding method
  • Sectional zebra fish breeding method

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Experimental program
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Effect test

Embodiment 1

[0041] Embodiment 1 zebrafish culture method in stages

[0042] A zebrafish culture method in stages, comprising a female fish culture stage, a broodstock breeding stage, an embryo hatching stage, a larvae culture stage, a juvenile fish culture stage, a juvenile fish culture stage, and an adult fish culture stage; wherein, the mother fish culture stage, broodstock culture stage The light intensity of the breeding stage, larvae culture stage, juvenile fish culture stage, juvenile fish culture stage, and adult fish culture stage is 1000lux, the light intensity of the embryo hatching stage is: 1500lux, and the light-to-dark ratio is light time: dark time=16h: 8h, water hardness 140mg·L -1 , the pH is 7.7;

[0043]Female fish culture stage: put the female fish in the flowing water culture system for cultivation, the water temperature is controlled at 25°C; feed fresh live brine shrimp 3mL / 10L in the morning and evening; the culture density is 12 fish / 10L;

[0044] Broodstock bre...

Embodiment 2

[0070] A zebrafish culture method in stages, comprising a female fish culture stage, a broodstock breeding stage, an embryo hatching stage, a larvae culture stage, a juvenile fish culture stage, a juvenile fish culture stage, and an adult fish culture stage; wherein, the mother fish culture stage, broodstock culture stage The light intensity in the breeding stage, larvae cultivation stage, juvenile fish cultivation stage, juvenile fish cultivation stage, and adult fish cultivation stage is 1000lux, and the light-to-dark ratio is light time: dark time=14h:8h, water hardness 130mg·L -1 , pH is 7.5;

[0071] Female fish culture stage: put the female fish in the flowing water culture system for cultivation, the water temperature is controlled at 24°C; feed fresh live brine shrimp 2.5mL / 10L in the morning and evening; the culture density is 8 fish / 10L;

[0072] Broodstock breeding stage: Put 6 male and 6 female zebrafish broodstock into a glass aquarium at night, control the water ...

Embodiment 3

[0097] A zebrafish culture method in stages, comprising a female fish culture stage, a broodstock breeding stage, an embryo hatching stage, a larvae culture stage, a juvenile fish culture stage, a juvenile fish culture stage, and an adult fish culture stage; wherein, the mother fish culture stage, broodstock culture stage The light intensity of the breeding stage, larvae cultivation stage, juvenile fish cultivation stage, juvenile fish cultivation stage, and adult fish cultivation stage is 1500lux, and the light-to-dark ratio is light time: dark time=12h:12h, and the water hardness is 160mg·L -1 , pH is 7.9;

[0098] Female fish culture stage: put the female fish in the flowing water culture system for cultivation, the water temperature is controlled at 26°C; feed fresh live brine shrimp 3.5mL / 10L in the morning and evening; the culture density is 14 fish / 10L;

[0099] Broodstock breeding stage: Put 6 male and 6 female zebrafish broodstock into a glass aquarium at night, contr...

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Abstract

The invention relates to a sectional zebra fish breeding method and belongs to zebra fish breeding methods. The method comprises a female fish breeding stage, a parent fish propagation stage, an embryo incubation stage, a larva fish breeding stage, an advanced fry breeding stage, a juvenile fish breeding stage and a grown fish breeding stage, wherein the light intensity of the female fish breedingstage, the parent fish propagation stage, the embryo incubation stage, the larva fish breeding stage, the advanced fry breeding stage, the juvenile fish breeding stage and the grown fish breeding stag is all 1000-1500 lux; the light darkness ratio is a lighting time; the dark time is 16 h:8 h, 14 h:10 h or 12 h:12 h; the water hardness is 130-150 mg*L<-1>; the pH value is 7.5-7.9. In addition, onthe premise that the breeding method provided by the invention does not use artificial synthesis substance which can effect growth and development of zebra fish, or antibiotics which can interfere test results and resistance of the zebra fish, a great amount of test fishes meeting experiment requirements can be bred, and the bred fishes are high in survival rate and low in death rate.

Description

technical field [0001] The invention belongs to the field of cultivating zebrafish, and in particular relates to a stage-by-stage culturing method of zebrafish. Background technique [0002] Water quality benchmarks are an indispensable scientific basis and theoretical basis for ecological environment testing departments to formulate water quality standards, evaluate water quality and conduct water quality management. Water quality standards for aquatic organisms include acute aquatic organisms water quality standards and chronic aquatic organisms water quality standards, which are an important basis for formulating water quality standards and acute aquatic ecological risk assessment. The Aquatic Biological Benchmark takes the aquatic organisms inhabiting the aquatic ecosystem as the protection target, and aims to maintain the integrity of the structure and function of the aquatic ecosystem and biodiversity. [0003] Zebrafish has the advantages of short growth cycle, stron...

Claims

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Application Information

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IPC IPC(8): A01K61/10A01K61/17
CPCA01K61/10A01K61/17Y02A40/81
Inventor 冯承莲廖伟吴丰昌金小伟刘娜符志友王琛闫振飞刘大庆白英臣
Owner CHINESE RES ACAD OF ENVIRONMENTAL SCI
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