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Detection kit for cholesterol content and detection method

A detection kit and cholesterol detection technology, which is applied in biochemical equipment and methods, measuring devices, microbial determination/inspection, etc., can solve the problems of low sensitivity and specificity of serum TC, difficult storage of natural enzymes, and narrow linear range, etc. Achieve the effects of improving detection sensitivity, enhancing fluorescence recovery intensity, and wide linear range

Active Publication Date: 2019-07-30
CHINA UNIV OF PETROLEUM (EAST CHINA)
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the sensitivity and specificity of the existing enzymatic detection of serum TC are relatively low, and the linear range is narrow, which cannot meet the detection of high-concentration cholesterol. When detecting high-concentration cholesterol, it must be diluted by a certain number of times. Moreover, natural enzymes are not easy to store. variability

Method used

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  • Detection kit for cholesterol content and detection method
  • Detection kit for cholesterol content and detection method
  • Detection kit for cholesterol content and detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] A cholesterol content detection kit, comprising the following reagents: potassium hydroxide ethanol solution, n-hexane aqueous solution, Triton X-100 isopropanol solution, solution A composed of Hepes buffer and sodium chloride, cholesterol oxidase solution, oxidation Praseodymium solution and fluorescently labeled DNA probe solution; Wherein, the concentration of the potassium hydroxide ethanol solution is 8.9mmol / L; The volume ratio of normal hexane and water in the normal hexane aqueous solution is 2:1; The Triton X- The volume ratio of Triton X-100 and isopropanol in 100 isopropanol solution is 2:3; The concentration of buffer in described solution A is 10mmol / L, and pH is 7.6, and the concentration of sodium chloride in described solution A is 150mmol / L; the concentration of the cholesterol oxidase solution is 5mg / mL; the concentration of the praseodymium oxide solution is 66 μmol / L; the concentration of the fluorescently labeled DNA probe solution is 6nmol / L, and t...

Embodiment 2

[0064] The difference between the cholesterol content detection kit provided in this example and Example 1 is that solution A is acetate buffer.

[0065] The method for detecting the cholesterol content of the above four serum samples to be tested by using the cholesterol content detection kit provided in this example is the same as that in Example 1, and the test results are shown in Table 2.

[0066] The detection result of table 2 embodiment 2 to 4 kinds of serum samples to be tested

[0067] Sample serial number Cholesterol standard content (μmol / L) Cholesterol detection content (μmol / L) Recovery rate(%) 1 4.40 3.71 84.32 2 6.90 5.84 84.64 3 11.50 9.65 83.91 4 12.30 10.47 85.12

Embodiment 3

[0069] The difference between the cholesterol content detection kit provided in this example and Example 1 is that the solution only includes Hepes buffer, and no sodium chloride is added.

[0070] The method for detecting the cholesterol content of the above four serum samples to be tested by using the cholesterol content detection kit provided in this example is the same as that in Example 1, and the test results are shown in Table 2.

[0071] Sample serial number Cholesterol standard content (μmol / L) Cholesterol detection content (μmol / L) Recovery rate(%) 1 4.40 4.21 95.68 2 6.90 6.47 93.77 3 11.50 11.01 95.74 4 12.30 11.85 96.34

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Abstract

The invention provides a detection kit for cholesterol content and a detection method, and belongs to the technical field of detection of cholesterol content. The detection kit is high in sensitivityand specificity and large in linearity range, and in addition, all reagents in the detection kit for cholesterol content are stable and easy to store. The detection kit for cholesterol content comprises the following reagents such as the potassium hydroxide ethanol solution, the aqueous normal hexane solution, the Triton X-100 isopropanol solution, the solution A, the cholesterol oxidase solution,the praseodymium oxide solution and the fluorescently-labeled DNA probe solution; the solution A comprises a buffering solution.

Description

technical field [0001] The invention belongs to the technical field of cholesterol content detection, and in particular relates to a cholesterol content detection kit and a detection method. Background technique [0002] At present, in laboratories at home and abroad, commercial enzymatic kits are usually used to detect total cholesterol (TC) in serum on automatic instruments. The principle of enzymatic detection of serum TC is that cholesterol esterase hydrolyzes cholesterol ester into free cholesterol, cholesterol oxidase oxidizes cholesterol to generate 4-encholestanone and hydrogen peroxide, hydrogen peroxide reacts with peroxidase Phenolic or aniline compounds and 4-aminoantipyrine or its analogs undergo oxidation and condensation reactions to produce quinoneimine compounds with a maximum absorption wavelength of 500-600 nm (Trinder reaction). Within a certain concentration range, quinone The concentration of imine compounds is directly proportional to the serum TC con...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/60C12Q1/26G01N21/64
CPCC12Q1/60C12Q1/26G01N21/6486
Inventor 姜磊刘涵云冯卫华陈钰雪刘智宾杨丽敏
Owner CHINA UNIV OF PETROLEUM (EAST CHINA)
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