Portable thermal imaging immunoassay method constructed based on photothermal and immunological functionalized liposome
An immune function and immune analysis technology, applied in the field of portable thermal imaging immune analysis, can solve the problems of low photothermal conversion efficiency and poor biocompatibility, and achieve the effect of simple operation, simple equipment, and portability
Inactive Publication Date: 2019-07-30
FUZHOU UNIV
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Problems solved by technology
In many existing portable bioanalyses that use temperature as a sensing signal, gold nanoparticles and ferroferric oxide nanoparticles are traditionally used as photothermal beacon molecules and are usually used as beacons for generating photothermal signals. Molecules, but poor biocompatibility and low light-to-heat conversion efficiency limit the further application of temperature-based sensing in the field of portable bioanalysis
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Embodiment 1
[0033] 1. Ti 3 C 2 Synthesis of quantum dots:
[0034]0.5 g Ti 3 AlC 2 The powder was slowly added to 10 mL of hydrofluoric acid with a mass fraction of 48%, and stirred at 60°C for 20 hours, and the resulting suspension was centrifuged at 3500 rpm for 10 minutes, and the precipitate was washed with deionized water until the washing solution was neutral. sex. Subsequently, 0.1 g of the above-mentioned washed product was added to 20 mL of 25% tetramethylammonium hydroxide aqueous solution, and stirred at room temperature for 24 hours, and the resulting suspension was centrifuged at 3500 rpm for 15 minutes to obtain The precipitate was washed with 100 mL of deionized water, and the obtained neutral product was dissolved in water at a mass ratio of 1:150, and under the protection of an argon atmosphere, the mixed solution was ultrasonicated in a water bath for 20 hours (ultrasonic power 200W) . Finally, the obtained suspension was centrifuged at 5000 rpm for 30 minutes, and...
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Abstract
The present invention relates to a portable thermal imaging immunoassay method constructed based on photothermal and immunological functionalized liposome, and belongs to the technical field of sensorinstrument construction and biochemical analysis. The method comprises: using Ti3C2 quantum dots as photothermal beacon molecules and the liposome as a loading container to prepare the photothermal functionalized liposome; then combining immune molecules to realize immunological functionalization of the photothermal liposome; and finally, in a designed portable thermal imaging analysis device, using an immunoreaction to achieve specific recognition of the target. Under the excitation of 808 nm infrared light, the photothermal liposome introduced by the immunoprobe can efficiently convert thelight energy into the heat energy, so that the temperature of the detection system can increase; and the increased temperature can be monitored by a portable infrared thermal imaging camera, so thatquantitative detection can be performed on the target. According to the method provided by the present invention, the device used is simple, portable, and easy to operate, and a simple, sensitive andstable method is provided for portable detection.
Description
technical field [0001] The invention relates to a portable thermal imaging immune analysis method constructed by photothermal and immune functional liposomes, belonging to the technical field of sensor instrument construction and biochemical analysis. Background technique [0002] With the development of society, people pay more and more attention to environmental monitoring, food safety and disease diagnosis. Many detection methods based on optics, chromatography, mass spectrometry and electrochemical have been established. These detection methods have the advantages of accuracy, sensitivity and stability. . However, expensive equipment and complicated operation limit its further application in rapid detection and daily life detection, especially in remote areas. Therefore, it is of great significance to design a detection method with simple equipment, easy operation, stability and practicality. [0003] As a common physical phenomenon, light-to-heat conversion has applic...
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IPC IPC(8): G01N33/53G01N25/48
CPCG01N25/4873G01N33/53
Inventor 唐点平蔡国能余镇重张莉佳
Owner FUZHOU UNIV