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A method for extracting total cardiac glycosides from oleander leaves

A technology of oleander leaves and cardiac glycosides, which is applied in the field of chemical separation, can solve the problems of low yield of total cardiac glycosides and low utilization rate of raw materials, and achieves the effects of high application value, improved utilization rate and good extraction effect.

Active Publication Date: 2021-06-11
上海泰坦科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] In view of the deficiencies in the prior art, the object of the present invention is to provide a method for extracting total cardiac glycosides from oleander leaves, to solve the problem that the current extraction process has a low utilization rate of raw materials and the yield of total cardiac glycosides lower question

Method used

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Effect test

Embodiment 1

[0049] This embodiment provides a method for extracting total cardiac glycosides from oleander leaves

[0050] (1) After 100 g of oleander leaves were dried and pulverized at 85° C., leached with 1000 mL of ethanol for 4 times at 65° C. to obtain an extract;

[0051] (2) Concentrate the extract until the alcohol content is lower than 10% and filter it. At a temperature of 60°C, the filtrate uses 500 mL of a mixed solvent of n-butanol, chloroform and ethanol in a volume ratio of 5:3:1. Extraction in the presence of sodium to obtain the extract;

[0052] (3) Concentrating the extract obtained in step (2) to a content of less than 20% n-butanol, then standing at 5°C for gel analysis, and drying the supernatant at 90°C to obtain total cardiac glycosides.

Embodiment 2

[0054] This embodiment provides a method for extracting total cardiac glycosides from oleander leaves

[0055] (1) After 100 g of oleander leaves were dried and crushed at 88°C at high temperature, they were leached with 1200mL ethanol for 6 times at 63°C to obtain an extract;

[0056] (2) Concentrate the extract until the alcohol content is lower than 10% and then filter. At a temperature of 70°C, the filtrate uses 800 mL of a mixed solvent of n-butanol, chloroform and ethanol with a volume ratio of 6:4:1. Extraction in the presence of potassium to obtain the extract;

[0057] (3) Concentrating the extract obtained in step (2) to a content of less than 20% n-butanol, then standing at 10°C for gel analysis, and drying the supernatant at 120°C to obtain total cardiac glycosides.

Embodiment 3

[0059] This embodiment provides a method for extracting total cardiac glycosides from oleander leaves

[0060] (1) After 100 g of oleander leaves were dried and pulverized at 90°C at high temperature, they were leached with 500mL ethanol for 3 times at 30°C to obtain an extract;

[0061] (2) Concentrate the extract until the alcohol content is lower than 10% and then filter. At a temperature of 80°C, the filtrate uses 300 mL of a mixed solvent of n-butanol, chloroform and ethanol with a volume ratio of 6:3:1. Extraction in the presence of sodium to obtain the extract;

[0062] (3) Concentrating the extract obtained in step (2) to a content of less than 20% n-butanol, then standing at 0° C. for gel analysis, and drying the supernatant at 80° C. to obtain total cardiac glycosides.

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Abstract

The invention provides a method for extracting total cardiac glycosides from oleander leaves, the method comprising the following steps: (1) leaching oleander leaves with lower alcohol to obtain an extract; (2) extracting the extract After concentrating and filtering, the filtrate is extracted with a mixed solvent of n-butanol, chloroform and ethanol to obtain an extract; (3) the extract obtained in step (2) is concentrated and allowed to stand for gel analysis, and the supernatant is dried to obtain total cardiac glycosides The method provided by the present invention, by carrying out one-time extraction with lower alcohol, then using the method for secondary extraction of n-butanol, chloroform and ethanol mixed solution, greatly improves the utilization rate of raw material oleander leaves, the total cardiac glycosides The yield is relatively high. Generally, for 100g of oleander leaves, the quality of the total cardiac glycosides finally obtained can reach about 20g, the highest can reach more than 24g, and the content can reach about 0.5mol / g at the same time. The extraction effect is good and the application value is high. high.

Description

technical field [0001] The invention belongs to the field of chemical separation, and relates to a method for extracting total cardiac glycosides from oleander leaves. Background technique [0002] Cardiac glycosides are mainly used clinically to treat cardiac insufficiency. Commonly used clinically are digitalis, digoxin, deacetyllanatoside C and oleanthin K, which have similar pharmacodynamic characteristics, but have differences in pharmacokinetics and action strength, which can be determined according to the action The speed of occurrence and the length of maintenance time are classified. [0003] The currently commonly used cardiac glycoside extraction method generally uses 70% to 80% ethanol as a solvent, and then performs targeted extraction according to the composition of the raw material. If it contains a lot of lipid impurities, it is generally degreased with petroleum ether or gasoline before extraction; if it contains a lot of chlorophyll, it is generally possi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/24A61P9/00A61K127/00
CPCA61K36/24A61K2236/333A61P9/00
Inventor 谢应波张庆张华徐肖冰罗桂云张维燕
Owner 上海泰坦科技股份有限公司
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