Use of phage AH10-Phage-QY01 in preparation of medicines for treating or preventing and controlling bacterial diseases in aquaculture

A phage and application technology, applied in the field of prevention and control of bacterial diseases in aquaculture, can solve problems such as strong drug resistance, difficulty in prevention and control, easy to obtain and difficult to treat sepsis, etc., and achieve high specificity, strong antibacterial effect, and good inhibitory effect Effect

Inactive Publication Date: 2019-08-06
SHANGHAI OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] Aiming at the current bacterial fish disease, especially the highly pathogenic bacterial disease Aeromonas Hydrophila (Aeromonas Hydrophila) infection caused by sepsis is easy to get and difficult to treat, strong drug resistance and diffi

Method used

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  • Use of phage AH10-Phage-QY01 in preparation of medicines for treating or preventing and controlling bacterial diseases in aquaculture
  • Use of phage AH10-Phage-QY01 in preparation of medicines for treating or preventing and controlling bacterial diseases in aquaculture

Examples

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Embodiment

[0024] Example: Screening, Isolation and Purification of Aeromonas hydrophila Phage AH10-Phage-QY01

[0025] Taking advantage of the ubiquity of phages and their high specificity to host bacteria, in the following examples, the corresponding phages were screened from the natural environment for various aquaculture pathogenic bacteria and used to treat the aquaculture bacteria caused by the pathogenic bacteria sick. The specific steps are:

[0026] Take 50ml of sampling sample and centrifuge at 4000rpm / min for 30min.

[0027] Aspirate 10ml of the supernatant, filter and sterilize with a 0.22um filter.

[0028] Add 25ml of 3 times concentrated LB liquid medium into a 200ml Erlenmeyer flask, cool to room temperature, add the filtrate into the Erlenmeyer flask, add 1ml of host bacteria, and add 0.5M calcium chloride to a final concentration of 0.1mM at the same time, Cultivate at 28 degrees for 12-14 hours.

[0029] Take out 3ml of the culture solution and put it into a 5ml st...

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Abstract

The invention discloses a use of aeromonas hydrophila phage AH10-Phage-QY01 in preparation of medicines for treating or preventing and controlling bacterial diseases in aquaculture, and belongs to thetechnical field of prevention and control of bacterial diseases in aquaculture. The phage AH10-Phage-QY01 is preserved in China Center for Type Culture Collection and has the preservation number of CCTCC NO:M2019109. The invention also includes a use of the phage AH10-Phage-QY01 in preparation of medicines for bacterial septicemia caused by aeromonas hydrophila. The phage AH10-Phage-QY01 can be used alone or in combination with antibiotics. The universality of the phage and high specificity to host bacteria are used, the aeromonas hydrophila phage AH10-Phage-QY01 is screened for the first time from natural environment for pathogenic bacteria, and is used for inhibiting and cracking the pathogenic bacteria, treating or preventing and controlling bacterial septicemia and other bacterial fish diseases caused by the pathogenic bacteria.

Description

technical field [0001] The invention belongs to the technical field of prevention and control of aquaculture bacterial diseases, and in particular relates to Aeromonas hydrophila phage AH10-Phage-QY01 and its use in preparing medicines for treating or preventing and controlling aquaculture bacterial diseases. Background technique [0002] Bacterial fish disease is the main disease of freshwater aquaculture industry. During the epidemic period, it not only leads to the death of a large area of ​​cultured species and fish eggs, but also makes the cultured products lose their commercial value, causing serious economic losses. Bacterial fish diseases are easy to get, difficult to treat, and easy to produce drug resistance. At present, due to the extensive use of antibiotics, various aquatic pathogens are becoming more and more drug-resistant, and the prevention and control of bacterial fish diseases is becoming more and more difficult. . [0003] Sepsis caused by Aeromonas Hydr...

Claims

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Application Information

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IPC IPC(8): C12N7/00A61K35/76A61P31/04C12R1/92
CPCA61K35/76A61P31/04C12N7/00C12N2795/00021C12N2795/00032
Inventor 吕利群徐宁王浩
Owner SHANGHAI OCEAN UNIV
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