Nucleic acid aptamer affinity monolithic column capable of specifically recognizing F2 toxins and preparing method of nucleic acid aptamer affinity monolithic column

Abstract

The invention belongs to the field of analysis chemistry, and particularly relates to a nucleic acid aptamer affinity monolithic column capable of specifically recognizing F2 toxins and a preparing method of the nucleic acid aptamer affinity monolithic column. An organic-inorganic hybrid silica gel polymer which is rich in sulfydryl on the surface and is formed through photo-initiation polymerization of a multi-sulfydryl functional monomer, polyhedral oligomeric silsesquioxanes (POSS), a pentaerythritoltetrakis(3-mercaptopropionate) cross-linking agent and the like is taken as a substrate, anaptamer with sulfydryl is bonded with rich alkenyl on the substrate through a sulfydryl-alkenyl click reaction, an anti-mycotoxin nucleic acid aptamer with sulfydryl is modified on POSS nanometer particles with high coverage density, and thus the monolithic column with an affinity effect on the F2 toxins is prepared. The monolithic column substrate has the advantages of highly-cross-linked structure and multiple acting sites, the nucleic acid aptamer with the function of specific recognition on the F2 toxins can be efficiently loaded and fixed on the POSS-based monolithic column with high density, the structure is stable, the method is simple and quick, and the monolithic column can be used for efficient specific recognition and separation of the F2 toxins.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and in particular relates to a nucleic acid aptamer affinity monolithic column for specifically recognizing F2 toxin and a preparation method thereof. Background technique [0002] The monolithic column is a continuous porous monolithic material formed by in-situ polymerization or immobilization in the column, and the surface of the monolithic material can be derivatized according to actual needs. Affinity chromatography is a chromatography method established based on the principle of affinity adsorption and dissociation between biomolecules. The solid-phase carrier is coupled to prepare a specific affinity adsorbent, which is then used as a stationary phase to fill the chromatographic column for the purification of the target substance, showing great potential for development in the field of monolithic column research. [0003] Nucleic acid aptamer is a short-chain DNA or RNA sequence that h...

AI Technical Summary

Problems solved by technology

[0007] At present, the research on nucleic acid aptamer-modified monolithic columns mainly focuses on inorganic silica gel monolithic columns and organic polymeric monolithic columns, while there are few studies on organic-inorganic hybrid monolithic columns.

Images