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Method for decolorizing tremella root polysaccharide

A polysaccharide and Tremella technology, which is applied in the field of polysaccharides, can solve the problems of difficulty in decolorization, black polysaccharides, and damage to the structure of polysaccharides, and achieves the effect of solving the problem of low decolorization rate, improving the polysaccharide polysaccharide rate, and not destroying the polysaccharide structure.

Pending Publication Date: 2019-08-09
INST OF AGRI ENG TECH FUJIAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Preliminary tests of the present invention have found that tremella polysaccharides with a concentration of more than 0.2% have a high viscosity at normal temperature, which brings difficulties to decolorization; activated carbon adsorption is used to decolorize polysaccharides, and activated carbon powder is combined with the polysaccharide solution of tremella , could not be completely separated, but turned the polysaccharides into black; using hydrogen peroxide to decolorize the polysaccharides of the ear pedicles, it is easy to oxidize and decompose the polysaccharides, thereby destroying the polysaccharide structure, and there are safety problems; a single macroporous resin adsorbs the polysaccharides of the ear pedicles, and the decolorization rate of the polysaccharides is less than 85%, and there are significant differences in the decolorization effect of macroporous resins with different polarities and pore sizes
Therefore, the polysaccharide decolorization method in the prior art cannot solve the problem that its polysaccharides are not easy to decolorize and the polysaccharide decolorization rate is low due to problems such as excessive viscosity of Tremella fungus pedicle

Method used

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  • Method for decolorizing tremella root polysaccharide
  • Method for decolorizing tremella root polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Tremella fungus polysaccharide decolorization method, it may further comprise the steps:

[0040] S1 ear pedicle crude polysaccharide extraction:

[0041] S11 The first water leaching: After cleaning the fungus pedicles, cut them into 0.8cm×0.8cm cubes, and the amount of water added is 13 times that of the fungus pedicles; after adding water, keep stirring for 30 minutes at a temperature of 90°C , filtered through a 200-mesh sieve to obtain the filtrate and filter residue for the first time;

[0042] S12 The second water leaching: Add the obtained filter residue to water and extract it under ultrasonic waves to obtain the second filtrate. The amount of water added is 3 times that of the filter residue; after adding water, extract in a water bath at 80°C with a power of 400W for 10 minutes , to obtain the second filtrate through 200 mesh sieve filtration.

[0043] S13 Alcohol precipitation and freeze-drying: After combining the first filtrate and the second filtrate in...

Embodiment 2

[0048] Tremella fungus polysaccharide decolorization method, it may further comprise the steps:

[0049] S1 ear pedicle crude polysaccharide extraction:

[0050] S11 The first water extraction: After cleaning the fungus pedicles, cut them into 0.8cm×0.8cm cubes, and the amount of water added is 18 times that of the white fungus pedicles; after adding water, keep stirring for 45 minutes at a temperature of 100°C , filtered through a 200-mesh sieve to obtain the filtrate and filter residue for the first time;

[0051] S12 The second water leaching: Add water to the obtained filter residue and extract it under ultrasonic waves to obtain the second filtrate. The amount of water added is 6 times that of the filter residue; after adding water, extract in a 95°C water bath for 25 minutes with a power of 600W ultrasonic , to obtain the second filtrate through 200 mesh sieve filtration.

[0052] S13 Alcohol precipitation and freeze-drying: After the first filtrate and the second filt...

Embodiment 3

[0057] Tremella fungus polysaccharide decolorization method, it may further comprise the steps:

[0058] S1 ear pedicle crude polysaccharide extraction:

[0059] S11 The first water extraction: After cleaning the fungus pedicles, cut them into 0.8cm×0.8cm cubes, and add 15 times the amount of water to the fungus pedicles; after adding water, keep stirring for 40 minutes at a temperature of 95°C , filtered through a 200-mesh sieve to obtain the filtrate and filter residue for the first time;

[0060] S12 The second water leaching: Add water to the obtained filter residue and extract it under ultrasonic waves to obtain the second filtrate. The amount of water added is 5 times that of the filter residue; after adding water, extract in a water bath at 90°C with a power of 500W for 18 minutes , to obtain the second filtrate through 200 mesh sieve filtration.

[0061] S13 Alcohol precipitation and freeze-drying: After the first filtrate and the second filtrate are combined into a ...

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Abstract

The invention relates to polysaccharide, in particular to a method for decolorizing tremella root polysaccharide. The method comprises the following steps of S1, extraction of root crude polysaccharide for standby application; S2, purification of the root crude polysaccharide, wherein the obtained root crude polysaccharide is subjected to chromatography by a polyamide resin chromatography column to obtain a polyamide purified polysaccharide solution; S3, secondary purification, wherein the obtained polyamide purified polysaccharide solution is added into HP-20 macroporous resin for adsorption,and centrifugal separation is carried out to obtain a root decolorization polysaccharide solution; S4, concentration and drying, wherein the obtained root decolorization polysaccharide solution is subjected to decompression concentration and vacuum freezing in sequence to obtain the tremella root polysaccharide. The method for decolorizing the tremella root polysaccharide has the advantage of improving the decolourization rate of the tremella root polysaccharide by using a double standing adsorption method of polyamide combined with the macroporous resin.

Description

technical field [0001] The invention relates to a polysaccharide, in particular to a method for decolorizing the tremella fungus pedicle polysaccharide. Background technique [0002] Tremella fungus peduncles are distributed in the junction of Tremella fruiting bodies and culture medium. They are yellow in color and are by-products of Tremella fungus commercial mushrooms after harvesting. They account for more than 10% of Tremella fungus biomass and have not been effectively utilized at present. Tremella fungus pedicles are rich in polysaccharides. Extracting polysaccharides from Tremella fungus pedicles can effectively utilize ear pedicle resources and develop functional foods, medicines, cosmetics and other industries. However, the tremella fungus pedicle is yellow, and the yellow pigment combines with the polysaccharide during the polysaccharide extraction process, which affects the purity and color of the polysaccharide and restricts the application of the product. [0...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 李怡彬吴俐汤葆莎陈君琛
Owner INST OF AGRI ENG TECH FUJIAN ACAD OF AGRI SCI