PCR primer combination for Passiflora edulia Sims cucumber mosaic virus and detecting method

Abstract

The invention provides a PCR primer combination for Passiflora edulia Sims cucumber mosaic virus and a detecting method. Particularly, the PCR primer combination comprises 3 Passiflora edulia Sims cucumber mosaic virus coat protein specific primers and 2 Passiflora edulia Sims general reference gene EF1a specific primers. Through the design of the 3 Passiflora edulia Sims cucumber mosaic virus coat protein specific primers, 3 specific sites of the virus can be detected at the same time, and occurrence of false positive is avoided; by using the two Passiflora edulia Sims EF1a specific primers,the Passiflora edulia Sims cDNA synthesis quality can be detected, and occurrence of false negative is avoided. The whole detection process can be completed within 4 hours, PCR result detection can beconducted by using 1% agarose, an expensive fluorescent quantatitive PCR instrument is not needed, and the PCR primer combination has the advantages of being fast, economical, high in sensitivity andhigh in stability.

Description

technical field [0001] The invention relates to the field of plant virus detection, in particular to a combination of PCR primers and a detection method for passion fruit cucumber mosaic virus. Background technique [0002] passion fruit( Passifloraspp .) Also known as passion fruit, egg fruit, as a fruit that can be eaten fresh and processed, it is widely planted in tropical and subtropical regions all over the world. [0003] Passion fruit is susceptible to a variety of viral diseases, among which cucumber mosaic virus ( Cucumber mosaic virus, CMV) are the most common. CMV is a very serious RNA-like virus disease. The virus can reach any part except the growth point. Passion fruit has been propagated through cuttings or grafting for a long time, which can easily cause the spread of large-scale diseased plants. In terms of chemical planting, usually the whole plant can only be directly destroyed if the virus disease is found in passion fruit. Therefore, the key to the p...

AI Technical Summary

Problems solved by technology

[0004] Although passion fruit CMV-infected plants can show obvious characteristics, the onset of CMV requires certain climatic conditions, and it is difficult to identify diseased plant materials

The use of PCR technology for CMV virus detection has the characteristics of strong specificity, high sensitivity, fast and stable, and low detection cost. There have been successful cases in various plants, but so far, there have been no related reports on passion fruit.

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