Peony molecular breeding method

A molecular breeding and peony seed technology, applied in the field of plant cultivation, can solve the problems of slow growth and long peony breeding cycle, and achieve the effects of promoting seed germination, shortening time and speeding up the process.

Active Publication Date: 2019-08-23
LUOYANG INST OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, under natural conditions, most ornamental peonies are infertile and need to be propagated asexually by cuttings and grafting; oil peonies are fertile, but grow slowly from seed sowing

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0031] Example 1:

[0032] In late August 2018, the seed coats of the harvested new peony seeds were removed, treated with NaClO with a mass concentration of 2% on an ultra-clean workbench for 8 minutes, washed with sterile water 5 times, and then placed on a shaker (about 100rpm ), change the water every 20 minutes and repeat three times to obtain detoxified peony seeds. Soak the detoxified tree peony seeds in containing 300mg / L gibberellin (GA 3 ) In a sterile solution for 24h, then evenly put it on MS medium, seal the cling film, cultivate in the dark in an artificial climate box at 22°C for three days, and then place it in a light incubator to alternate light / dark for 30 days, alternately During the cultivation, the cultivation time was 16h and 8h in light and dark, respectively, the light intensity was 3000 Lux, and the humidity was 70%. The peony seeds grow cotyledons after 30 days of cultivation.

[0033] The Agrobacterium LBA4404 containing the p3301 recombinant vector wa...

Example Embodiment

[0039] Example 2: In late February 2019, the seeds stored in the refrigerator at 4°C were soaked for 1 hour and then classified and screened, and the seeds deposited on the bottom were selected for subsequent experiments. Remove the seed coat, treat it with 2% NaClO on a clean bench for 10 minutes, wash it with sterile water 6 times, then place it on a shaker (about 100 rpm), change the water every 20 minutes, repeat three times, Obtain detoxified peony seeds. Soak the detoxified peony seeds in containing 300mg / L GA 3 In the sterile solution for 24h, then evenly put it on MS medium, seal the fresh-keeping film, cultivate in the dark in an artificial climate box at 22℃ for three days, and then place it in a light incubator for 30 days with alternating light / darkness , The light and dark culture time are 16h and 8h respectively in the alternate culture, the light intensity is 3000 Lux, and the humidity is 70%. The peony seeds grow cotyledons after 30 days of cultivation.

[0040]...

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PUM

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Abstract

The invention relates to a peony molecular breeding method. The peony molecular breeding method comprises peeling peony seeds, performing virus-free treatment with sodium hypochlorite, soaking the virus-free seeds in sterile gibberellin solution for 24 h, and inoculating the seeds onto an MS (Murashige-Skoog) culture medium for dark culture and induction for 3 days and then light/dark alternate culture for 30 days; after cotyledons emerge, cutting up and co-culturing the cotyledons with agrobacterium tumefaciens LBA4404 containing exogenous genes for 2 days, washing off the agrobacterium tumefaciens, inducing transgenic resistance callus culture and differentiation to obtain transgenic seedlings, further identifying the transgenic seedlings through GUS (beta-glucuronidase) staining, strengthening positive transgenic seedlings in a strengthening culture medium, and lastly, performing rooting culture to complete peony breeding. The peony molecular breeding method significantly shortens the time for seed germination, improves the transgenic effects and provides a new technique and method for peony molecular breeding.

Description

technical field [0001] The invention relates to the technical field of plant cultivation, in particular to a peony molecular breeding method. Background technique [0002] Peony belongs to the peony group of the genus Paeoniae in the family Paeoniaceae. It is a small perennial deciduous shrub and is a traditional Chinese flower. Peony not only has ornamental value and medicinal value, but also has important food value. However, under natural conditions, most ornamental peonies are infertile and need to be propagated asexually through cuttings and grafting; oil peonies are fertile, but grow slowly from seed sowing to seedlings, resulting in a relatively long breeding cycle for peonies. long. At present, there is no complete and efficient molecular breeding method of peony transgenic at home and abroad. Contents of the invention [0003] In order to overcome the deficiencies in the prior art, the present invention provides a method for molecular breeding of tree peony, wh...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001A01H4/005
Inventor 张琳陈为张浩玉张柯
Owner LUOYANG INST OF SCI & TECH
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