Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Peony molecular breeding method

A molecular breeding and peony seed technology, applied in the field of plant cultivation, can solve the problems of slow growth and long peony breeding cycle, and achieve the effects of promoting seed germination, shortening time and speeding up the process.

Active Publication Date: 2019-08-23
LUOYANG INST OF SCI & TECH
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, under natural conditions, most ornamental peonies are infertile and need to be propagated asexually by cuttings and grafting; oil peonies are fertile, but grow slowly from seed sowing to seedlings, resulting in a relatively long breeding cycle for peonies. long
At present, there is no complete and efficient molecular breeding method for peony transgenics at home and abroad.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] In late August 2018, the seed coats of the new peony seeds harvested were removed, treated with NaClO with a mass concentration of 2% on the ultra-clean workbench for 8 minutes, washed 5 times with sterile water, and then placed on a shaker (about 100rpm ), change the water every 20min, repeat three times, and obtain detoxified peony seeds. Soak detoxified peony seeds in 300mg / L gibberellin (GA 3 ) in a sterile solution for 24 hours, then evenly placed on the MS medium, sealed with plastic wrap, and cultivated in the dark for three days in an artificial climate box at 22°C, and then placed in a light incubator for 30 days in light / dark alternately. During cultivation, the light and dark cultivation time were 16h and 8h respectively, the light intensity was 3000 Lux, and the humidity was 70%. After 30 days of cultivation, the peony seeds grow cotyledons.

[0033] Agrobacterium LBA4404 containing the p3301 recombinant vector was inoculated in YEP liquid medium containin...

Embodiment 2

[0039] Example 2: In late February 2019, the seeds stored in the refrigerator at 4°C were sorted and screened after soaking for 1 hour, and the seeds that settled at the bottom were selected for subsequent experiments. The seed coat was removed, treated with 2% NaClO for 10 min on the ultra-clean bench, washed 6 times with sterile water, then placed on a shaker (about 100 rpm), changed the water every 20 min, and repeated three times. Get detoxified peony seeds. Soak detoxified peony seeds in 300mg / L GA 3 24 hours in a sterile solution, then evenly placed on the MS medium, sealed with plastic wrap, and cultured in an artificial climate box at 22°C for three days in the dark, and then placed in a light incubator for 30 days in light / dark alternating culture , during alternate culture, the light and dark culture time were 16h and 8h respectively, the light intensity was 3000 Lux, and the humidity was 70%. After 30 days of cultivation, the peony seeds grow cotyledons.

[0040]...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a peony molecular breeding method. The peony molecular breeding method comprises peeling peony seeds, performing virus-free treatment with sodium hypochlorite, soaking the virus-free seeds in sterile gibberellin solution for 24 h, and inoculating the seeds onto an MS (Murashige-Skoog) culture medium for dark culture and induction for 3 days and then light / dark alternate culture for 30 days; after cotyledons emerge, cutting up and co-culturing the cotyledons with agrobacterium tumefaciens LBA4404 containing exogenous genes for 2 days, washing off the agrobacterium tumefaciens, inducing transgenic resistance callus culture and differentiation to obtain transgenic seedlings, further identifying the transgenic seedlings through GUS (beta-glucuronidase) staining, strengthening positive transgenic seedlings in a strengthening culture medium, and lastly, performing rooting culture to complete peony breeding. The peony molecular breeding method significantly shortens the time for seed germination, improves the transgenic effects and provides a new technique and method for peony molecular breeding.

Description

technical field [0001] The invention relates to the technical field of plant cultivation, in particular to a peony molecular breeding method. Background technique [0002] Peony belongs to the peony group of the genus Paeoniae in the family Paeoniaceae. It is a small perennial deciduous shrub and is a traditional Chinese flower. Peony not only has ornamental value and medicinal value, but also has important food value. However, under natural conditions, most ornamental peonies are infertile and need to be propagated asexually through cuttings and grafting; oil peonies are fertile, but grow slowly from seed sowing to seedlings, resulting in a relatively long breeding cycle for peonies. long. At present, there is no complete and efficient molecular breeding method of peony transgenic at home and abroad. Contents of the invention [0003] In order to overcome the deficiencies in the prior art, the present invention provides a method for molecular breeding of tree peony, wh...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001A01H4/005
Inventor 张琳陈为张浩玉张柯
Owner LUOYANG INST OF SCI & TECH
Features
  • Generate Ideas
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com