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Ascorbic acid carbon nanodot fluorescent probe, preparation method and application thereof

A technology of ascorbic acid and fluorescent probes, which is applied in the field of chemical measurement, can solve the problems of expensive equipment, limitations, and high detection costs, and achieve the effect of omitting the pretreatment process, avoiding the use of large instruments, and simple production

Inactive Publication Date: 2019-08-23
WEST ANHUI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods often have problems such as expensive equipment, high detection cost, and complicated sample pretreatment, which are greatly limited in practical application and are difficult to be widely used in environmental monitoring and control.

Method used

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  • Ascorbic acid carbon nanodot fluorescent probe, preparation method and application thereof
  • Ascorbic acid carbon nanodot fluorescent probe, preparation method and application thereof
  • Ascorbic acid carbon nanodot fluorescent probe, preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0029] The method steps of the preparation of the ascorbic acid carbon nano-dot fluorescent probe that the present invention proposes are as follows:

[0030] S1: Add polyethyleneimine to the reaction kettle containing ascorbic acid solution, seal it, and then heat it to react, wherein the molecular weight of polyethyleneimine is 600, the concentration of ascorbic acid is 1.5μg / mL, the concentration of polyethyleneimine and ascorbic acid The molar ratio is 1:1, and the reaction conditions are heating temperature 180°C and reaction time 6h;

[0031] S2: Cool the solution after the reaction in S1 to room temperature to obtain a brown liquid, and perform membrane filtration on it, wherein the membrane filtration is a needle filter of 0.45 μM;

[0032] S3: Dialyze the solution filtered by the membrane in S2, wherein the dialysis condition is that the Dalton molecular weight of the dialysis bag is 1000, the dialysis time is 8 hours, and the ultrapure water is changed every 2 hours;...

Embodiment 2

[0035] The method steps of the preparation of the ascorbic acid carbon nano-dot fluorescent probe that the present invention proposes are as follows:

[0036] S1: Add polyethyleneimine to the reaction kettle containing ascorbic acid solution, seal it, and then heat it to react, wherein the molecular weight of polyethyleneimine is 10000, the concentration of ascorbic acid is 2.5μg / mL, the concentration of polyethyleneimine and ascorbic acid The molar ratio is 1:40, and the reaction conditions are a heating temperature of 220°C and a reaction time of 8 hours;

[0037] S2: Cool the solution after the reaction in S1 to room temperature to obtain a brown liquid, and perform membrane filtration on it, wherein the membrane filtration is a needle filter of 0.45 μM;

[0038] S3: Dialyze the solution filtered by the membrane in S2, wherein the dialysis conditions are that the Dalton molecular weight of the dialysis bag is 10000, the dialysis time is 12 hours, and the ultrapure water is ...

Embodiment 3

[0041] The method steps of the preparation of the ascorbic acid carbon nano-dot fluorescent probe that the present invention proposes are as follows:

[0042] S1: Add polyethyleneimine to the reaction kettle containing ascorbic acid solution, seal it, and then heat it for reaction, wherein the molecular weight of polyethyleneimine is 10000, the concentration of ascorbic acid is 2μg / mL, the molar ratio of polyethyleneimine and ascorbic acid The ratio is 1:20, and the reaction conditions are heating temperature 200°C and reaction time 7h;

[0043] S2: Cool the solution after the reaction in S1 to room temperature to obtain a brown liquid, and perform membrane filtration on it, wherein the membrane filtration is a needle filter of 0.45 μM;

[0044] S3: Dialyze the solution filtered by the membrane in S2, wherein the dialysis conditions are that the Dalton molecular weight of the dialysis bag is 1000, the dialysis time is 10 hours, and the ultrapure water is changed every 2.5 hour...

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Abstract

The invention discloses an ascorbic acid carbon nanodot fluorescent probe, a preparation method and application thereof. The preparation method comprises the following steps: S1, adding polyethyleneimine into a reaction kettle containing an ascorbic acid solution, performing sealing, and then performing heating to carry out a reaction; S2, cooling the solution after the completion of the reactionin S1 to be room temperature to obtain a brown liquid, and performing membrane filtration on the solution; S3, dialyzing the solution subjected to the membrane filtration in S2; and S4, performing freeze-drying on the dialyzed solution in S3 to obtain the ascorbic acid carbon nanodot fluorescent probe. The probe is sensitive and rapid to the response of mercury ions, a linear relationship betweenthe fluorescence quenching intensity and the mercury ion concentration is established, the quantitative detection of mercury ions is realized, and the detection limit is 22 nM. In addition, the probehas high selectivity to mercury ions, the stability is good, the probe can be preserved and used for a long term, the synthesis is simple, and thus the probe is beneficial to commercialized popularization and application.

Description

technical field [0001] The invention relates to the technical field of chemical measurement, in particular to ascorbic acid carbon nano-dot fluorescent probe, its preparation method and application. Background technique [0002] The content of heavy metal mercury in natural water generally does not exceed 1.0 μg / L. Mercury pollution in water mainly comes from industrial waste water discharged from mercury-producing factories and mines, metal mining and smelting, and mercury production departments, especially mercury in the chemical production process. emission. In the water bottom, inorganic mercury salts are transformed into highly toxic methylmercury and dimethylmercury under the action of methylcobalaminylmethionine transferase in microorganisms. Especially organic methyl mercury, which has strong lipophilicity, is easily absorbed by aquatic organisms, and eventually accumulates through the food chain and poses a serious threat to human health. In particular, it has gre...

Claims

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Application Information

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IPC IPC(8): C09K11/65C09K11/02B82Y20/00B82Y30/00B82Y40/00G01N21/64
CPCB82Y20/00B82Y30/00B82Y40/00C09K11/025C09K11/65G01N21/643G01N2021/6432G01N2021/6439
Inventor 闫叶寒陈丽娟郑玉赵丹刘仁勇
Owner WEST ANHUI UNIV
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